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St. Joseph's College New York *

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MISC

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Biology

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Dec 6, 2023

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docx

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17

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A Novel Approach to Combat Amebiasis: Testing Natural Solutions on Paramecium Multimicronucleatum Chris Trinidad
Trinidad 2 Purpose The purpose of this project is to compare the effect of natural remedies on the viability percentage of Paramecium multimicronucleatum .
Trinidad 3 Hypothesis If sulfur and Cinchona alkaloids are associated with the extermination of protozoa, then the combination of quinine and garlic will have the greatest effect on the viability percentages because the sulfur of the garlic and the anti-protozoal attributes of the quinine will exterminate the organisms.
Trinidad 4 Materials BSL-1 Lab Blender Refrigerator Gram scale Garlic extract ingredients/materials: 1. 24 g of peeled garlic cloves 2. Coffee filter 3. Glass jar Ginger extract ingredients/materials: 1. 24 g of peeled ginger 2. Coffee filter 3. Knife 4. Glass jar Quinine extract/tonic water ingredients: 1. 28 g of chopped Cinchona bark 2. 2 glass jars 3. Large glass pitcher 4. Coffee filter mL measuring cup Camera Stereoscope, if necessary 20 plastic petri dishes – 60mm x 15mm 200 individual organisms from Carolina Biological Supply: Paramecium multimicronucleatum (Paramecium, Living)
Trinidad 5 1-gallon jug of Carolina Biological Springwater (Media) : 1 gallon is equal to 3.8 liters. 8 pipettes 4 graduated cylinders in mL 1 box of medical-grade gloves Lab aprons (one apron for each person experimenting) Safety goggles (one goggle for each person experimenting) 1 roll of paper towels 10% bleach solution – one-gallon jug: 1 gallon is equal to 3.8 liters. Fine tip Sharpie or permanent marker 3M masking tape Sticky note 1 piece of paper 1 large bucket Hydrion Spectral pH Paper Dispensers (pH 1.0 to 14.0)
Trinidad 6 Procedure 1. Adult supervision will be maintained throughout the experiment and appropriate lab procedures will be adhered to. Proper handling and disposal of the Paramecium will be demonstrated in the experiment. A designated supervisor and parent will be present throughout the whole duration of the experiment. Experimenters will be using gloves, aprons, and safety goggles. 2. Aerate culture upon delivery as soon as possible. This will be done by adult sponsor or designated supervisor. 1. Put on gloves, aprons, and goggles. 2. Wipe laboratory counters with the bleach solution using paper towels. Throw paper towels away after use in the garbage. 3. Unscrew the lids of each of the cultures. 4. Aerate the culture to help replace the oxygen depleted during shipping. To do this, use a plastic pipette. Place the pipette tip into the culture water and squeeze the bulb, bubbling air into the water. Withdraw the pipette and release the bulb, allowing it to refill with air. Repeat 4 more times. 5. Replace the lids, but do not screw tight. Leave it in an area that is around 22º Celsius. 3. Make extracts at home, prior to laboratory work. This will be done by a parent. 1. Tonic water/quinine extract 1. Measure the 28 grams of Cinchona bark using the scale, then combine with the 675 mL of spring water in a sterilized, lidded glass jar. Use the mL measuring cup for the spring water. Shake to combine. Refrigerate for 72 hours, shaking occasionally, at least once per day.
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