18f Fludeoxyglucose Lab Report

The purpose of this experiment was to determine the relationship between tail spine length and hemoglobin levels as well as the relationship between tail spine length and heart rate. The concentration of the hemoglobin in Daphnia is dependent on the oxygen available to them.

The purpose of this lab was to identify unknown bacteria cultures using various differential tests, and my unknown bacteria is #17. The identification of these unknown cultures was accomplished by separating and differentiating possible bacteria based on specific biochemical characteristics. Whether the tests performed identified specific enzymatic reactions or metabolic pathways, each was used in a way to help recognize those specifics and identify the unknown cultures. The differential tests used to identify the unknown cultures were Gram stain, Catalase, Mannitol Salt Agar (MSA), Blood Agar, Novobiocin, Coagulase, and DNAse (Alachi, 2007).

Bacteria are ubiquitous; they can be found on the skin, in the soil, and inside the body. Because of the very nature of this ubiquity, it is important to be able to determine between different strains of bacteria. An example of this is determining the causative agent for a disease so that the patient will be treated with the appropriate antibiotics. It may be important to determine the bacteria in a certain region, because like with enteric bacteria, it is normal to find them in the digestive tract as they are in a symbiotic relationship with our bodies in this area; however, they also cause opportunistic infections in places outside of the digestive tract to our detriment, such as with a urinary tract infection. Some strains of bacteria are common to nosocomial infections, and identifying these bacteria as such helps create the guidelines for healthcare workers in antiseptic technique. All of the morphology and characteristics of each strain of bacteria help us to better understand the role of bacteria in the body as well as helps us understand how they can cause illness, and what treatment regimen to set in place. In lab this semester, a sample of unknown

Make three exposures using given technical factors on a phantom knee in PA position . Include saline bags in exposures 1 and 2 to demonstrate patient soft tissue thickness.

Again, label 7 1.5ml tubes 0 thru 6. Place 15μl of each serially diluted extract into its corresponding labeled tube. Next add 465μl of media into each tube. Then 60μl of Alamar blue in each tube. Finally add an additional 60μl of cells (adjusted to 10,000 cells/20 μl). Vortex each tube for 5 seconds. Now, take 3 different samples 190μl samples of concentration 0 and put it in Wells A2, B2, and C2. Repeat this step again by taking 3 more different 190μl samples of concentration 1 and putting it in wells A3, B3, C3. It should be noted that it is important to vortex each 1.5μl tube again be-fore putting it into the 96 well plate. Contin-ue this same procedure consecutively for the re-maining concentrations.

Identifying Organism 6C Introduction: This report details the steps taken and processes used to discover the identity of the unknown organism given to me on Tuesday, November 28th, 2017. With all of the knowledge and skills gained over the semester, in class and in lab, I was able to positively identify my unknown organism. The objective of these labs being, that I successfully utilize the tests and procedures taught during the course to correctly identify my organism and to be able to explain the reasoning behind my tests and results.

Triple Sugar Iron Agar test, there was a gas production seen in the media. The media was yellow slant and yellow butt indicating glucose, lactose and/or sucrose fermentation with acid accumulation in slant and butt. For sulfur reduction, it was negative since it did not turn black in color indicating no sulfur was reduced.

Hypothesis: The purpose of this experiment was to investigate the question will different food sources affect the level of activity of detoxification enzymes in bean beetles? The class alternate hypothesis is different food sources will affect the level of activity of the detoxification enzymes in bean beetles. The null hypothesis is the different food sources will not have any effect on the level of activity of the detoxification enzymes in bean beetles. Experimental design: The independent variables in this experiment were the types of beans (bean 1 was mung beans and bean 2 was adzuki beans) and enzymes assays used.

The sole purpose of this project was to identify an unknown bacteria sample #7. Many tests were carried out to determine what this unknown was. Aside from a microbiology lab, understanding and identifying various organisms are important in disease processes, pharmaceutical arenas, and even in the industrial field. Proper lab techniques, including aseptic technique were used throughout the process of identification.

The purpose of the Unknown Lab is to practice and implement all that was learned in this microbiology lab this semester about the different test used in identification of an unknown bacteria and to effectively identify an unknown bacterium.

The unknown project was an experiment where the student was given a petri dish of unknown bacteria. Tests were performed on it to identify the specific species. The purpose of the experiment was to learn about the identifying tests, and procedures in the identification of specific microbes. The reason the master plate was used to create a working plate is so that if the working plate becomes contaminated, one can resort back to the master plate for the pure strain of the bacteria and create a new working plate. The purpose of the first procedure, the gram stain, was to be able to dye and then distinguish gram negative and gram-positive cells on a smear. The second procedure, the citrate test is used to see if the bacteria can use citrate as

2. When 2.00 g of NaOH were dissolved in 49.0 g water in a calorimeter at 24.0 ˚C, the temperature of the

Approximately 0.05 g of 9-fluorenol, 250mg of chromic oxide resin, and 2 mL of toluene were used for the reaction solution. The CrO3 resin consisted of little brown solid balls. The reaction solution was slowly heated to 130°C. The reaction solution slowly turned light yellow as it was being heated. 1M standards for 9-fluorenol and 9-fluorenone were used. The reaction mixture had a strong gas-like odor while it was filtered through the Hirsch funnel. After evaporating the solvent using a rotary evaporator, the crude product that was left was yellow and solid. When hexane was added the yellow solid formed into a yellow liquid.

Review of systems is negative for any known opthalmologic, ENT, cardiovascular, respiratory, gastrointestinal, genitourinary, musculoskeletal, skin, psychiatric, endocrine, hematologic, or immunologic problems.

When we write, “drill a 3/8” diameter, 1” depth hole in the center of the dowel”, the reality is a lot different. First, we need to find 3/8” tap size that is specifically designed for wood. Then, we need to find the center point of the dowel and figure out how to drill a hole perfectly centered. The process took us 2 hours in lab to figure out the most efficient and effective way to do such simple task. Because of time constrain, we decided to finish the system later in the day.