250 Milliliter Lab Report

For the second part of the experiment I cut the cheesecloth into four pieces and folded them so that it was four layers thick. I placed one piece of cheesecloth into the funnel and measured 60mL of soil using the 100mL to help measure the soil and poured that into the funnel. Taking beaker number one I poured the contents into the funnel and let that filter into beaker number five. I used the same technique as above and I repeated the same thing to beakers number two through four and poured them into beakers number six through eight. Once this was done I observed beakers five through eight and wrote down my observations in Table 1.

In this project, C. Elegans are hermaphrodite worms that will be used since they are easy to maintain in lab, as well as have short life cycles. The gene that the project attempted to knockdown in C. Elegans with RNAi treatment is the unc-22 gene. RNAi disrupts gene expression in the presence of double stranded RNA (dsRNA) that is complementary to target gene sequence. The unc-22 gene codes for a muscle protein called twitchin in wild-type worms. The Unc-22 is required for muscle regulation and maintenance in C.Elegans. To verify that the RNAi treatment worked, would check the unc-22 mRNA levels in the worms, in addition to phenotype observation.

First, all of the equipment was obtained and placed on the table. The necessary equipment was weighed using a top loader balance, which used grams as its unit of measurement. The test tube, which held the samples, was filled with water from a plastic squirt bottle. A piece of filter paper, which had been folded several times, was placed over a funnel over an Erlenmeyer flask to filter the mixture in the test tube. The test tube mixture was poured onto the filter paper.

The mean voltage of the battery terminals while connected to the identification resistors is presented in Figure ‎4 12. These samples have been pulled out from the voltage sensor of the PEB panel. The voltage decreased as expected from 12.53 to 12.5 during first 20 seconds of connection to the

October 17, 18, and 19, samples were collected from multiple sites along the BSR. The class was split into groups, and samples were collected from seven separate locations along the river and WWTP. There was also a sample collected by the S which is located between sites four and five. For each of these sites, there were ten groups from other labs that also collected a sample from the BSR. At site two of the river, the location included multiple sources of possible contamination. A drainage site was located 200 yards upstream, along with a small PVC drainage pipe next to the collection site. Not only was there drainage running into the river, the site was under a bridge, and contained other trash scattered throughout the area. The

First, a 100 mL graduated cylinder was obtained and filled with 35 mL of water. A pipet was used to attain a more accurate amount of liquid. The water was then poured into a beaker, which was weighed on an analytical balance. Next, an Alka-Seltzer tablet was obtained and the weight measured using the same balance the weight of the beaker was measured on. When both masses were recorded, the tablet was dropped into the water. The liquid was swirled to allow for the tablet to dissolve completely. After the fizzing had stopped, the beaker was once again weighed and the mass was recorded. Each step was repeated seven more times for a total of eight trials. However, with each trial the liquids added to the beaker changed. In each new trial, an additional 5 mL of vinegar was added and 5 mL of water was taken away. Thus, beaker one had 0 mL of vinegar and 35 mL of water; beaker 2 had 5 mL of vinegar and 30 mL of water; beaker 3 had 10 mL of vinegar and 25 mL

The aqueous solution on the bottom layer was drained into the 150mL beaker. 4. Steps 2 and 3 was repeated two more times and the final mix was with 5mL of water, then the aqueous solution on the bottom layer was drained into the 150mL beaker. 5.

phenylethyl alcohol to change the membrane permeability of Gram-negative bacteria (13). Growth on the plate indicated a positive result. Lack of growth on the EMB plate also suggested a Gram-positive bacterium. This was because the medium contained a methylene blue dye that hindered the growth of such bacteria (14). The high sodium content of the MSA plate created a high osmotic pressure that inhibited the growth of species that cannot withstand the pressure (15).

Studies show that unwashed or lightly rinsed vegetables can harbor pathogenic bacteria and have been implicated in numerous foodborne infections. The objective of this experiment was to obtain a quantitative viable plate count of enteric bacteria present in a sample of broccoli sprout water. To enumerate the number of bacterial colonies present in the sample, we performed serial dilutions of broccoli water spread onto MacConkey Agar plates. MacConkey Agar is a selective and differential media. It is used to select enteric bacteria and differentiate lactose-fermenters from non-lactose fermenters. Utilizing this process illustrated the potential amount of foodborne illness causing bacteria present on foods, specifically fresh broccoli

Chemistry is composed of many molecules and changes of properties in the matter of science. The elements on the periodic table arranged from metal to non metals, plays an important role when it comes to making a compound and mixture. Each of the element have their own unique atomic mass which matters when it comes to organizing the periodic table and when there is a calculation. There are lots of importance about the atomic mass of each element that will be discussed later in this essay. An experiment performed using the mass of zinc and copper has determined whether which element have excess remaining. Will there be a difference if an alkali metal was used?

In this experiment, there are no significant ethical considerations as we had not used any living organisms. There were no extreme safety concerns, however there are still precautions that needed to be taken. Cobalt chloride is very toxic and is irritating to the skin so when handling the cobalt chloride solution, wear lab coats and safety goggles and have all hair tied up. Handle the solution with care to try and prevent the solution from coming into contact with skin. If it does spill onto skin, wash it off with water and soap. Do not touch your eyes when handling this substance to prevent it from entering your eyes.

A filter paper was added to the Büchner funnel. The faucet, which acted as a vacuum source, was turned on. Before pouring the reaction mixture into the filter, the filter paper was wetted using distilled water. A pair of tongs were used to transfer the reaction into the filter from the beaker. After most of the reaction was in the filter, the beaker was rinsed twice with 5mL of distilled water each time. The contents of the rinse in the beaker were emptied into the filter. After the filtration was complete and the dark residue of the aluminum can pieces was separated from the clear reaction mixture, the filtrate was transferred into another clean 250mL beaker. Then the filter flask was rinsed with 10mL of distilled water and added to the filtrate in the new reaction beaker.

In order to investigate the expression of miR-365b-3p in normal cells and lung cancer cells, we used real-time PCR to measure the expression of miR-365b-3p in various cells. Results showed that the expression of miR-365b-3p was significantly lower in lung cancer cells compared with that in normal cells (Figure 1). After transfection, the expression of miR-365b-3p in COLO 668 cell line was successfully upregulated by miR-365b-3p mimic and inhibited by miR-365b-3p inhibitor (Figure 2A). Cell viability assay showed that miR-365b-3p suppressed cell viability while miR-365b-3p inhibitor promoted cell proliferation (Figure 2B). Furtherly,

In this paper, the author want to know whether human genetic patents affect subsequent scientific research and product development. They use the administrative data on successful and unsuccessful patent applications filed with the USPTO and then link the exact gene sequences required for each application with subsequent scientific research and business investment data. By using these data, they found patented genes have more valuable prior to being patented than non-patented genes. They used two quasi-experimental approaches to text their hypothesis. In the end, they found both of the approaches suggest that on average gene patents have had no effect on follow-on innovation. “This evidence of selection motivates two

Methods: Due to experimental error in previous labs, we did not obtain the DNA of our original taxa. We then chose to use DNA sequences supplied by the TA from the 2016 and 2017 school year. We picked six fungal taxa obtained from differing parts of English Yew Trees, including its fruit, bark, and leaves. Using the DNA sequences given, we copied the sequence into the BLAST system found on blast.ncbi.gov, making sure to add “fungi” into the organism box and to search for somewhat similar sequences. Once the program obtained the results, we scrolled down to the first result that gave a genus and species name, with an “ITS” in the description. Using the information given in the results, we filled in the information on Table 2, including seq