4.4. Descriptive Statistics and Trends of Process Parameters
Mean, standard deviation, CV, maximum and minimum values of process parameters from the 10 batches studied are shown in table 6 (see page 14 -15).
The variability (CV) of the studied process parameters ranged from 1.2% -108.3%. Buffer analyte chemistry and charcoal mixing duration had CV less than 3 % (Range 1.2% -2.6%) and showed very little variation. The starting volume, volume post diafiltration and final volume post aseptic filtration had CV ranges from 9.6%-11.1 % indicating that the variability of plasma volume at three important stages of the process was not high. Process parameters during diafiltration such as volumetric concentration factor (VCF), membrane protein loading, process flux and number of buffer exchanges had CV values between 11.6 % and 21.1 %. The throughput (volume filtered per filter) for all filter types (M10, M90, Keystone, bag filters and 0.2 µm filters) exhibited variability (CV) in the range of 24.9 % to 47.8 %. Freeze duration post Aresoil addition, freeze duration post Aerosil removal and mix duration post Aerosil addition, all three factors involved with the delipidation showed variability greater than 30% (range 30.7%-108.3 %).
The in-process parameters were analyzed for trends by plotting time series graph (see page 22 -25) there was no significant trends in most of the parameters studied except for starting plasma volume, which showed a gradual increasing pattern over
Procedure: I used a ruler, thermometer, and scale to take measurements. I used a graduated cylinder, short step pipet, scale, and ruler to determine volume and density. I used a volumetric flask, graduated pipet, pipet bulb, scale, and glass beaker to determine concentrations and densities of various dilutions.
In the gel filtration step, we began with a slurry of Bio-Gel P-100 beads suspended in 20 mM phosphate buffer (Equilibration buffer) and an upright column with a stopcock. We added enough of the Bio-Gel P-100 beads to the column until we reached a height of 4.7 cm, making sure not to allow the column to run dry and that the top of the beads column is flat. We then added the sample, a mixture of Blue Dextran (2 mg/ml, 2 MDa), Hemoglobin (2 mg/ml), Bovine Serum Albumin (2 mg/ml), and Yellow Food Coloring (5 μl/ml, ~500 Da) (Sheffield, 37). This was followed by another ~1 ml of equilibration
With all solutes set at a concentration of 5.00 mg/ml and the MWCO set at 20, filtration stopped at 60 minutes, and the projected completion was 100 minutes. The residue analysis indicated all solutes present in the dialysis membrane. The filtrate concentrations for all solutes was 0.00 mg/ml. With all solutes set at a concentration of 5.00 mg/m and the MWCO set at 50, the filtration completed in 40 minutes. The residue analysis indicated all solutes present in the dialysis membrane. The filtrate concentration for NaCl was 4.81 mg/ml, and 0.00 mg/ml for all remaining
3) Compute limits for the sample mean X around μ=12 such that, as long as a new sample mean is within those limits, the process will be considered to be operating satifactorily. If X exceeds the upper limit or if X is below the lower limit, corrective action will be taken. These limits are refferred to as upper and lower control limits fro quality control purposes.
Me and my lab partner, obtained a mixture of a un known proportion from the instructor and then flow the guide line in our lab manual to separate the mixture by applying the separation method motioned in our lab manual pages 33-40 . In this experiment, the separation methods were decantation,
1.5mL of phosphoric acid including 3-4 boiling chips were also added to the 25mL flask. The short path distillation apparatus was set up as shown in Figure 1. A heating mantle was used to heat up the 25mL flask. The solution was distilled to the receiving flask until a small amount of liquid remained in the initial RBF flask. At this point the presence of thick grey smoke pulling over into the entire apparatus was observed. The apparatus was then left to cool down. Through the use of pasture pipette, the aqueous layer from the distilled solution was drawn out. Sodium carbonate was then added to the remaining organic solution in order to check the pH and to verify the basicity of the solution. The aqueous layer was again drawn out from the solution. Next, 0.5g of sodium sulfate was added to the remaining organic layer and was swirled until the liquid appeared to be dry and clear. The alkenes were transferred into a clean 10mL flaks using another clean pasture pipe. The apparatus from the first distillation was rinsed off with
Using the graduated cylinder, measure 20mLs of the stock sucrose solution and 180mL of water to create a 3% sucrose solution and place it into the 250mL beaker (beaker #2). Place bags #1‐3 (red, blue, yellow) into beaker 2 and bag #4 (green) into beaker 1. Allow the bags to sit for one hour. After allowing the bags to sit for one hour, remove them from the beakers carefully open the bags, noting that often times the tops may need to be cut as they tend to dry out. Measure the solution volumes of each dialysis bag using the empty 250 ml beaker.
One dialysis tube was submerged in each beaker. Osmosis was allowed to occur for 5 minutes and then all of the tubes were removed from the water. The tubes were dried off and measured on the triple beam scale. The mass was taken and recorded for all three tubes. I then placed the tubes back into their respective solutions. The process was repeated four times for each tube in 5 minute increments, and then the materials were disposed of. The rate of diffusion of water in each solution was
The purpose of this experiment is to familiarize oneself with the general procedures determining a partition coefficient at the microscale level and learn in weighing milligram quantities of materials on an electronic balance, the use of automatic pipets, the use of transfer pipet, and the use of a vortex mixer. Also, to familiarize oneself with extraction
2. The different concentrations of ONPG solutions and buffer solutions were incubated in the water bath at 37°C for 5 minutes.
The following Diluted Albumin (BSA) Standards have been prepared using Bovine Serum Albumin (BSA) Standard Solution (2.0mg/ml) and the Diluent Phosphate Buffered Saline (PBS) having
The table below represents the average samples processed per hour and per step, based off the minimum, average, and maximum samples processed on exhibit 4. Separation numbers are half of the other process’s sample numbers because only 50% of samples go to separation.
The boiling tubes will be used to hold the juice and the 1 % DCPIP solution. I will use boiling tubes because in the preliminary test I used a test tube and I found that the test tube was too small and was hard to shake the mixture therefore I will be using boiling tubes as the are slightly
* Introduces the construction and use of statistical process control (SPC) charts and an understanding of the relationship between SPC and conformance quality.
Six plastic cups were obtained and labeled 0.2 M, 0.4 M, 0.6 M, 0.8 M, and 1.0 M. Next, each of the six dialysis tubes were knotted on one end and filled with the sucrose samples, and then tied off. These samples were then dried by patting with a paper towel, weighed and placed into their corresponding cups. The mass of each sample was recorded in table 2 on the data sheet. Each cup was then