What Factors Affect the Rate of Photosynthesis in Living Leaves?

Have you ever really wondered how different variables can affect how plants go through photosynthesis? Well, in this experiment, the purpose was to see how various environmental conditions can affect the overall photosynthetic capacity of a specific plant. The factors, light, darkness, cold, and heat were applied to see how the different components would affect the photosynthesis on spinach plants. Each group was given a different factor to test. Out group was given the light factor. The hypothesis for this experiment is that when adding light as a factor, the light will affect the overall plant photosynthesis.

The purpose of this lab is to observe the effect of white, green, and dark light on a photosynthetic plant using a volumeter and followed by the calculation of the net oxygen production using different wavelengths color of white and green light, and also the calculation of oxygen consumption under a dark environment, and finally the calculation of the gross oxygen production.

You should point the curved tip of the syringe in the tonsil pocket to eliminate deep stones that cannot be seen. Keep the head down so that the stones come out of the mouth. The syringe should contain equal parts of warm water and 3 percent hydrogen peroxide.

1. Dip leaf boiling water for about 1 minute to soften it, stop further chemical changes and make the cell more permeable.

graduated cylinder with 50 ml of water; add 25 sunflower seeds and enough glass beads to reach 59 ml Remove the sunflower seeds and place them on a paper towel and empty the cylinder.

5.Soak the ten disks marked “A” in bleach. Remove the paper disks with a pair of tweezers and touch disks gently on a dry paper towel to blot excess fluid. Place one disk in each of the ten petri dishes, locating each disk five millimeters from the side of the dish at the twelve o’clock position.

Drosophila Melanogaster is a fruit fly that is commonly used for genetic studies (reference 2). It is an excellent organism for genetic studies because it is small, inexpensive and easy to culture. It occupies little space, and requires simple food (reference 3). Also, it completes its life cycle in about 10-14 days at 25ºC and It produces large numbers of offspring (reference 3).Moreover, it has abundance of heredity variations, and it has a small number of chromosomes which are easily located in the large salivary gland cells (reference 3).There are four important stages in a Drosophila's life cycle consist of the egg, larva, pupa, and adult. Both Drosophila male and female have noticeable features that distinguish them apart.

Procedures Pre-Lab: Gather all materials for the experiment. Read all instructions Part One: Creating the Model Gather the supplies needed for the creation of the model. A roll of the suggested dialysis tubing, a plastic water bottle, the air line tubing, the tubing connectors, a large empty milk carton, a rubber band, 2 large plastic sandwich bags, a fluid transfer siphon pump, and a pair of scissors. First, open the carton of milk by cutting the bottom of the empty milk carton.

From the tables seen above, it can be seen that the most successful trial, on average, was Trial 3. The smaller the mean value was, the quicker the leaf disks rose, and it can be seen that in Trial 1, leaf disks took an average of about 12 minutes to rise; in Trial 2, the leaf disks took

Have your syringe ready. Push the plunger all the way forward so there is no air gap.

The hypothesis for this part was that shorter wavelength of light will have a greater increase the consumption of CO_2 by the spinach leaves than the longer wavelengths. Four different colors of light were tested: purple, green, red, and yellow. The same steps as the first part were repeated for this part, but the only difference was using different colored lights instead of white light. The dependent variable for this experiment was the CO_2 concentration in the Testing Chamber, while the independent variable was light.

Roll a vial of insulin and forth between the palms of your hands several times before filling the syringe. This helps to redistribute all the particles that have settled

○ Pick up the gauze. Pour the saline solution over the gauze. Squeeze out the extra saline solution.

First, we collected spinach leaves and cut out 60 disks using cork borer and then placed them in a syringe. Then, we added sodium bicarbonate into the syringe leaving about a third of the syringe empty and replace the plunger to the syringe. We aspirated the leaves by pulling the plunger down

Remove the plunger (back) of the syringe. Insert the leaf discs into the barrel (main cylinder) of the syringe. Re-insert the plunger of the syringe, pushing forward until all air has been removed.