. What is the full form of RFLP?

Basic Clinical Laboratory Techniques 6E
6th Edition
ISBN:9781133893943
Author:ESTRIDGE
Publisher:ESTRIDGE
Chapter6: Basic Clinical Chemistry
Section6.4: Point-of-care Testing
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. What is the full form of RFLP?

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Step 1

Restriction enzymes are the endonucleases that cleave DNA specific locations. random DNA mutations change the restriction sites in the genome of organisms. Therefore, restriction digestion of DNA of different organisms of the same species produces restriction fragments of different lengths. This is called restriction fragment length polymorphism. 

 

Step 2

The restriction fragments produced by digestion of DNA of two or more organisms of the same species are observed for their length polymorphism. This reflects the evolutionary closeness of the organisms of the same or different populations. 

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