1. A. A mutation in E. coli is found that fails to induce the arabinose operon. Upon further investigation, it is found that this mutant also is unable to induce the lac operon. Which of the following single mutations could explain this phenotype (you may choose one or more answers a) Mutation in the promoter of the ara operon b) Mutation in the promoter of the lac operon c) Mutation in the operator of the lac operon d) Mutation in the AraC protein for the ara operon e) Mutation in the repressor of the lac operon f) Mutation in the Y gene for Lac permease g) Mutation in the gene for adenylate cyclase h) Mutation in the gene for CAP (or CRP) protein i) Mutation in the gene for rho factor j) None of the above

1. A. A mutation in E. coli is found that fails to induce the arabinose operon. Upon further investigation, it is found that this mutant also is unable to induce the lac operon. Which of the following single mutations could explain this phenotype (you may choose one or more answers a) Mutation in the promoter of the ara operon b) Mutation in the promoter of the lac operon c) Mutation in the operator of the lac operon d) Mutation in the AraC protein for the ara operon e) Mutation in the repressor of the lac operon f) Mutation in the Y gene for Lac permease g) Mutation in the gene for adenylate cyclase h) Mutation in the gene for CAP (or CRP) protein i) Mutation in the gene for rho factor j) None of the above

Name: 1.A. A mutation in E. coli is found that fails to induce the arabinos
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Description: 1.A. A mutation in E. coli is found that fails to induce the arabinose operon. Upon further investigation, itis found that this mutant also is unable to induce the lac operon. Which of the following single mutationscould explain this phenotype (you

Biology (MindTap Course List)

11th Edition

ISBN:9781337392938

Author:Eldra Solomon, Charles Martin, Diana W. Martin, Linda R. Berg

Publisher:Eldra Solomon, Charles Martin, Diana W. Martin, Linda R. Berg

Chapter14: Gene Regulation

Section: Chapter Questions

Problem 9TYU: A mutation that inactivates the repressor gene of the lac operon results in (a) the continuous...

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4) Suppose you have an E. coli in which the gene that codes for the lac repressor protein has been mutated in such a way that the protein produced can bind to both lactose and DNA (at its specific binding site in the lac operon promoter) simultaneously. What effect, if any, would this have on regulation of expression of the lac operon? Briefly (1 sentence) explain why you would predict the effect or lack of effect this mutation would have.
2) Attenuation of the trp operon is controlled by the formation of stem-loop structures in the mRNA of the trpl region. The attenuation function can be disrupted by mutations that alter the sequence of the repeat DNA regions 1-4 and prevent formation of the RNA stem loops. Describe the most likely effects on attenuation of each of the following mutation under the conditions specified. a. Mutation in region 1 and low levels of tryptophan: b. Mutation in region 2 and low levels of tryptophan: Mutation in region 2 and moderate levels of tryptophan (low enough to release the repression but enough to allow the ribosome to NOT pause): с. d. Mutation in region 3 and low levels of tryptophan: е. Mutation in region 3 and moderate levels of tryptophan (low enough to release the repression but enough to allow the ribosome to NOT pause): f. Mutation in region 4 and low levels of tryptophan:
1. A)Explain the mechanism by which the lac operon is sensitive to glucose levels (presence and absence of glucose). B)If glucose levels in the cell are high and lactose is available from the environment, what is the status of transcription of the lac operon genes? Explain your answer from both an evolutionary perspective and in terms of negative and positive regulation of the operon?
A mutation that inactivates transcription and translation from the regulatory gene of an inducible operon could result in: Group of answer choices A inactivation of RNA polymerase. B irreversible binding of the repressor to the operator. C three of these choices are correct D inhibition of transcription of the structural genes within the operon. E two of these choices are correct F transcription of the structural genes within that operon.
Answer the following questions: 1. Given the following genotypes, explain how the mutation (identified by a () superscript will affect E. coli grown in lactose medium. Will the lac operon be on or off? Will there be a complete set of gene products from the loc operon? What will be the implication of the missing gene product, if ever? Will the cell be able to survive in the lactose medium or not? a. p' oE Y b. p'o Y d. po v
IPTG can be used in the laboratory as a synthetic inducer of the lac operon, instead of lactose. Based on your knowledge of the regulation of the lac operon, predict with explanation whether these gene products (B-galactosidase, lactose Permease) will be produced. a IPOZY IPOZY b. IP'O'Z'Y/IPOZY" +IPTG B-galactosidase -IPTG Permease B-galactosidase Permease Only typed answer. Will upvote you immediately. Please help
28. Which statement does NOT explain why the lac operon would not be effectively regulated by an attenuation mechanism like the trp operon? 1.Lactose is a substrate for the lac operon protein products. 2.Products of lac operon enzymes are not used in protein synthesis. 3.Transcription and translation do not happen simultaneously for the lac operon. 4.Attenuation is a suitable regulatory mechanism for anabolic amino acid pathways. 5.All of these statements explain why regulation by attenuation would not be effective for the lac operon.
Read the following statements about operons, and mark the FALSE one. O a. Prokaryotes use operons frequently because they are an efficient use of resources O b. The expression levels of the genes in an operon can be regulated individually Genes in an operon are placed next to each other (physically) O d. The genes in an operon share a single promoter
. a. How many ribosomes are required (at a minimum)for the translation of trpE and trpC from a singletranscript of the trp operon?b. How would you expect deletion of the two tryptophan codons in the RNA leader to affect theexpression of the trpE and trpC genes?
1. The following are genotypes of merozygotes of E. coli with various combinations of lac operon mutations. Determine the phenotype with respect to beta-galactosidase (z), permease (y), and trans-acetylase (a) of each combination as U = uninducible, I = inducible, and C = constitutive. Choose from the following answers. i- p+ o+ z+ y- a+ / i+ p+ oc z- y- a+ Answer choices UII UIC UUU ICC CUC IUC CCU 2. The following are genotypes of merozygotes of E. coli with various combinations of lac operon mutations. Determine the phenotype with respect to beta-galactosidase (z), permease (y), and trans-acetylase (a) of each combination as U = uninducible, I = inducible, and C = constitutive. Choose from the following answers. i- p+ o+ z- y+ a+ / iS p+ o+ z- y+ a+ Answer choices ICC CCU UII IUC UIC CUC UUU
1. In E.coli transcription of the Lac Operon is known to be under Global control. For this operon explain the mechanisms of both negative and positive control (be sure to identify relevant regulatory molecules and operon regions). A. Positive Transcriptional Control: B. Negative Transcriptional Control: nodoubens besileroneD.8 2280019 pies8.) zenutost eupinUS C. If E.coli is grown in media containing both glucose and lactose when would you expect to see beta-galactosidase (catalyzes the hydrolysis of lactose) produced?
Explain how the following conditions will affect the regulation of the operon. (3 pts) Low cAMP levels; E. coli grown in a high lactose medium