1. An 9 kb circular plasmid is cut with the EcoRI restriction enzyme, and the reaction products an run on a DNA gel and stained with ethidium bromide. Bands of 1.0, 3.0 and 5.0 kb are seen. 2. When the same plasmid is cut with both the EcoRI and BamHI restriction enzymes (a double digest), bands of 1.0, 1.5, 3.0, and 3.5 kb are seen. From these results, it can be concluded that ( Select ] cuts within the V [ Select ] * kb [ Select ] v band 1.0
Bacterial Genomics
The study of the morphological, physiological, and evolutionary aspects of the bacterial genome is referred to as bacterial genomics. This subdisciplinary field aids in understanding how genes are assembled into genomes. Further, bacterial or microbial genomics has helped researchers in understanding the pathogenicity of bacteria and other microbes.
Transformation Experiment in Bacteria
In the discovery of genetic material, the experiment conducted by Frederick Griffith on Streptococcus pneumonia proved to be a stepping stone.
Plasmids and Vectors
The DNA molecule that exists in a circular shape and is smaller in size which is capable of its replication is called Plasmids. In other words, it is called extra-chromosomal plasmid DNA. Vectors are the molecule which is capable of carrying genetic material which can be transferred into another cell and further carry out replication and expression. Plasmids can act as vectors.
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Question 11
1. An 9 kb circular plasmid is cut with the EcoRI restriction enzyme, and the reaction products are
run on a DNA gel and stained with ethidium bromide. Bands of 1.0, 3.0 and 5.0 kb are seen.
2. When the same plasmid is cut with both the EcoRI and BamHI restriction enzymes (a double
digest), bands of 1.0, 1.5, 3.0, and 3.5 kb are seen.
From these results, it can be concluded that [ Select ]
cuts within the
V [ Select ]
kb
[ Select ]
v band
1.0
3.0
5.0](/v2/_next/image?url=https%3A%2F%2Fcontent.bartleby.com%2Fqna-images%2Fquestion%2F5c728d67-52ed-4447-8d34-fcfa8869e7dd%2F8e0e7e6b-6491-405e-bb18-d75400138c11%2Fkvg1mv9_processed.jpeg&w=3840&q=75)
![Question 11
1. An 9 kb circular plasmid is cut with the EcoRI restriction enzyme, and the reaction products are
run on a DNA gel and stained with ethidium bromide. Bands of 1.0, 3.0 and 5.0 kb are seen.
2. When the same plasmid is cut with both the EcoRI and BamHl restriction enzymes (a double
digest), bands of 1.0, 1.5, 3.0, and 3.5 kb are seen.
From these results, it can be concluded tha v[ Select ]
cuts within the
BamHI
[ Select ]
v kb
[Select
EcoRI](/v2/_next/image?url=https%3A%2F%2Fcontent.bartleby.com%2Fqna-images%2Fquestion%2F5c728d67-52ed-4447-8d34-fcfa8869e7dd%2F8e0e7e6b-6491-405e-bb18-d75400138c11%2Fm0mwl6k_processed.jpeg&w=3840&q=75)
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