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- Penicillin G potassium 5000 units Isotonic Sodium Chloride Solution ad 15 mL Sig. Nose drop Using soluble penicillin tablets, each containing 100,000 units of crystalline penicillin G potassium, explain how you would obtain penicillin G potassium needed in compounding the prescription.Date Sheet Extraction ODD EVEN ODD EVEN GROUP Single Extraction 1 Single Extraction 2 Multiple Extraction 1 Multiple Extraction 2 Weight of tea leaves 10.002 (g) 10.123 (g) 10.013 (g) 10.007 (g) Weight of evaporating dish + caffeine 123.689 (g) 124.334 (g) 121.815 (g) 126.523 (g) Weight of empty evaporating dish 123.513 (g) 124.147 (g) 121.614 (g) 126.246 (g) Weight of caffeine Post Lab Questions Explain the principles involved in extraction. What other organic compounds can be extracted from tea bags? Draw their structures and briefly mention their biological function/s. Why do you need to add sodium carbonate in the extraction process?Date Sheet Extraction ODD EVEN ODD EVEN GROUP Single Extraction 1 Single Extraction 2 Multiple Extraction 1 Multiple Extraction 2 Weight of tea leaves 10.002 (g) 10.123 (g) 10.013 (g) 10.007 (g) Weight of evaporating dish + caffeine 123.689 (g) 124.334 (g) 121.815 (g) 126.523 (g) Weight of empty evaporating dish 123.513 (g) 124.147 (g) 121.614 (g) 126.246 (g) Weight of caffeine 1. Identify the two layers in the procedure. In which part does caffeine can be extracted? 2. The partition coefficient K for a compound between dichloromethane and water is determined to be 56 : 1 (K=56). If 10.0 mg of the compound is dissolved in 50.0 mL of water and this solution extracted with 25.0 mL of dichloromethane, how many mg of the compound will remain in the water layer following the extraction procedure? Show you calculations
- Example of a Protein Purification Scheme: Purification of the Enzyme Xanthine Dehydrogenase from a Fungus Volume Total Total Specific Percent Fraction (mL) Protein (mg) Activity Activity Recovery 1. Crude extract 2. Salt precipitate 3. Ion-exchange chromatography |4. Molecular-sieve chromatography 5. Immunoaffinity chromatography 3,800 22,800 2,460 0.108 100 165 2,800 1,190 0.425 48 65 100 720 7.2 29 40 14.5 23 1.8 275 152.108 11 Calculate the specific activity of step#4. Note that percent recovery=% Yield.For 4-aminosalycilic acid please pick only the second choice phase 2 metabolite per handle present in the drug: OH H,N 4-Aminosalicylic acid OH إختر واحدة أو أكث OH OH NH, HN HO. OH HN HN он HO HO он HQ HO. HO. HN50 mg/50 ml kinetin stock is available You need 2.5 mg/l kn for Ms media calculate amount of kn is required? Explain the role of surface sterilising chemicals in aseptic culture initiation and function of sucrose in MS medium?
- Triple Sugar Iron slant for Staphylococcus Epidermidis , tell me the results I would get if I did this test?How much agarose need for 40 mls of 1.5%w/v gel15.5 R Erythromycin Lactobio- nate Dexamethasone Sodium Phosphate Glycerin 500 mg 100 mg 2.5 mL Sterile Water for Injec- tion ad M. ft. Ophthalmic Solution 100 mL (a) What is the percentage strength of erythromycin lactobionate in the prescription? (b) If glycerin has a specific gravity of 1.25, what is its concentration (w/v) in the prescription? 15. (a) 0.5% w/v erythromycin lactobionate (b) 3.125% w/v glycerin
- DQuestion 21 GC and HPLC were used in this course as: O synthetic techniques analytic techniques purification techniques O liquid-liquid extraction techniques D Question 22 Based on the boiling point of acetone (56° C), 3-methylbutylacetate (141°C), and 3-methyl-1- butanol (131°C), which compound will be the first to come off (elute) the GC column? O acetone O 3-methylbutylacetate O 3-methyl-1-butanolRx Clindamycin phosphate 4% w/v Propylene glycol 12.9% w/v Purified water 11% w/v Isopropyl alcohol ad 244 mL (Sp.G. = 0.880) Ft. clindamycin gel Sig.: Place thin layer onto affected areas How many grams of isopropyl alcohol are present in a clindamycin gel that has 76 mL of purified water? Assume negligible volume shrinkage as result of mixing these ingredients. Answer: 415.2 (please show all work) ANSWER IS ALREADY GIVEN PLEASE SHOW WORK IN DETAIL HOW IT COMES2 NDC 0186-0743-31 Keep containigdy closed. PRILOSec Protect from light and moisture Store between 15°C and 30°C (599 and 80°F) OMEPRAZOLE) 30 Delayed-Release Capsules 40 mg Rx only Manufactured for AstraZeneca LP PROSE Wington, DE 10850 40 By MERCK & CO. Whitehouse Shanon, NJ 06809, USA Name AstraZeneca 11 0.4 ESCOFF Figure 6-A2 Drug label for Prilosec 1) Read the drug label in Figure 6-A2. How many Prilosec (omeprazole) capsules would contain 0.32 g of the drug? 2) Using a formula estimate the body surface area of a person whose height is 6 feet 3 inches. and who weighs 200 pounds. |||| 0.5 USUAL ADULT DOSAGE: See package insert NDC 0054-3542-58 The PROSEC (omeprazole) Delayed Release Capsule should be swallowed whole and not opened, chewed, or crushed. 4) Order: Oncovin (vincristine sulfate) 2.5 mg po daily. The BSA is 1.63 m2 and the recommended dose is 1.4 - 2.0 mg/ m2. Is the prescribed dose in the safe dose range? 5) Indicate with an arrow a reading of 0.42 mL on the scale…