10. The concentration of haemoglobin in a blood sample was determined by spectrophotometry. A standard curve of the absorbance at 412 nm of several solutions of known haemoglobin concentrations was created. The data for the standard curve is shown below. a. Calculate the linearity coefficient (r), y-intercept and slope. b. What is the concentration (in pg/mL) of haemoglobin in your sample if the absorbance obtained at 412 nm was 0.303? Absorbance Concentration of standard solution (pg/ml) (412nm) 0.069 1 0.113 0.201 2 4 0.377 8 0.730 16

10. The concentration of haemoglobin in a blood sample was determined by spectrophotometry. A standard curve of the absorbance at 412 nm of several solutions of known haemoglobin concentrations was created. The data for the standard curve is shown below. a. Calculate the linearity coefficient (r), y-intercept and slope. b. What is the concentration (in pg/mL) of haemoglobin in your sample if the absorbance obtained at 412 nm was 0.303? Absorbance Concentration of standard solution (pg/ml) (412nm) 0.069 1 0.113 0.201 2 4 0.377 8 0.730 16

Fundamentals Of Analytical Chemistry

9th Edition

ISBN:9781285640686

Author:Skoog

Publisher:Skoog

Chapter25: Instruments For Optical Spectrometry

Section: Chapter Questions

Problem 25.6QAP

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