18. You want to express hemoglobin beta (HBB) in a bacteria model using a vector. You would liketo amplify the following gene by PCR.GTAGAATATG ATAAGCGAAA CTGCAAATCG CGTTTGGGGC GATACAAGTA GTGTACGCGGACCGCGCCGA GCGGGCTATG GTTTCTCCAC TATCAGTTCT TCTCCTGTCC CAGCGAAGTCGAGGTCCAGC CCTACGGTAC ATAACTAACA CGGTTTGAAG AAGATACGAT CTTACGAAGTAAAGAAAATT TGTAGTCAGC CCGGTTCGCT TGTGTCCAGC TAATCGATTG ATTGGCCCCAGCAGGCGAGA TGAACATAGT CATGCGCTGT CTAATAGCCC ATTTGACGTG TAGGTGGCGCTTTTATTTCT GAGGTGGAAA Ta) If the primers were both 20 nucleotides long, what are the sequences for both PCR primers that willamplify only the highlighted region? Be sure to label the 5' and 3' ends. (4 points)b) What is the length (in bp) of the entire region amplified by their primers (i.e. the amplicon length)? (Note:the spacing in the above sequence is intentionally uniform) (2 points)c) If you start with 300 copies template DNA how many copies would you expect to produce if you ran thePCR for 22 cycles? Show your work. (4 points)

PCR (Polymerase Chain Reaction) is a laboratory technique used to amplify specific regions of DNA.…

Answered: 18. You want to express hemoglobin beta…

Human Anatomy & Physiology (11th Edition)

11th Edition

ISBN:9780134580999

Author:Elaine N. Marieb, Katja N. Hoehn

Publisher:Elaine N. Marieb, Katja N. Hoehn

Chapter1: The Human Body: An Orientation

Section: Chapter Questions

Problem 1RQ: The correct sequence of levels forming the structural hierarchy is A. (a) organ, organ system,...

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What restriction enzyme (or enzymes) would you use to cut the following... (Gene of Interest is Bolded) 1 tctagagtca tgaaacaaca aaaacggctt tacgcccgat tgctgacgct gttatttgcg 61 ctcatcttct tgctgcctca ttctgcagca gcggcggcaa atcttaatgg gacgctgatg 121 cagtattttg aatggtacat gcccaatgac ggccaacatt ggaagcgttt gcaaaacgac 181 tcggcatatt tggctgaaca cggtattact gccgtctgga ttcccccggc atataaggga 241 acgagccaag cggatgtggg ctacggtgct tacgaccttt atgatttagg ggagtttcat 301 caaaaaggga cggttcggac aaagtacggc acaaaaggag agctgcaatc tgcgatcaaa 361 agtcttcatt cccgcgacat taacgtttac ggggatgtgg tcatcaacca caaaggcggc 421 gctgatgcga ccgaagatgt aaccgcggtt gaagtcgatc ccgctgaccg caaccgcgta 481 atttcaggag aacacctaat taaagcctgg acacattttc attttccggg gcgcggcagc 541 acatacagcg attttaaatg gcattggtac cattttgacg gaaccgattg ggacgagtcc 601 cgaaagctga accgcatcta taagtttcaa ggaaaggctt gggattggga agtttccaat 661 gaaaacggca actatgatta tttgatgtat gccgacatcg attatgacca tcctgatgtc 721 gcagcagaaa ttaagagatg gggcacttgg tatgccaatg…
Utilizing Hind III and EcoR V Restriction Enzyme with Pet41 and the following gene of interest... a tgaaacaaca aaaacggctt tacgcccgat tgctgacgct gttatttgcg 61 ctcatcttct tgctgcctca ttctgcagca gcggcggcaa atcttaatgg gacgctgatg 121 cagtattttg aatggtacat gcccaatgac ggccaacatt ggaagcgttt gcaaaacgac 181 tcggcatatt tggctgaaca cggtattact gccgtctgga ttcccccggc atataaggga 241 acgagccaag cggatgtggg ctacggtgct tacgaccttt atgatttagg ggagtttcat 301 caaaaaggga cggttcggac aaagtacggc acaaaaggag agctgcaatc tgcgatcaaa 361 agtcttcatt cccgcgacat taacgtttac ggggatgtgg tcatcaacca caaaggcggc 421 gctgatgcga ccgaagatgt aaccgcggtt gaagtcgatc ccgctgaccg caaccgcgta 481 atttcaggag aacacctaat taaagcctgg acacattttc attttccggg gcgcggcagc 541 acatacagcg attttaaatg gcattggtac cattttgacg gaaccgattg ggacgagtcc 601 cgaaagctga accgcatcta taagtttcaa ggaaaggctt gggattggga agtttccaat 661 gaaaacggca actatgatta tttgatgtat gccgacatcg attatgacca tcctgatgtc 721 gcagcagaaa ttaagagatg gggcacttgg tatgccaatg aactgcaatt ggacggtttc 781…
You design primers that will amplify a 400 bp fragment of the CAT gene and perform PCR. The 400 bp CAT fragment is shown below. The locations where the designed primers anneal are bolded and underlined. 5'GGATTTGGTCGTATTAGGCCTTCTTTTGCGTCGCCAGCCGAGCCACATCGCTCAGACACCATGGGGAAGTG AAGGTCGGAGTCAACGGATTTGGTCGTTTCTTTTGCGTCGCCAGCCGAGCCACATCGCTCAGACACATGGGGA AGTGAAGGTCGGAGTCAACACACTGGGTCATCCCGTCGCGCCTGGTCACCAGGGCTGCTTTTAACTCTGTTCG ACAGTCAGCCGCATCGGAAAGTGGATATTGTTGCCATCAATGACATTGGGCGCCTGGTCACCAGGGCTGCTTT TAACTCTGGAAAGTGGATATTGTTGCCATCAATGACCCCTTCATTGACCTCAACTACATGGTTTACATGTTCC AATAGATTCCACCCATGAGCCGAGCCACATCGCTCAG -3' a. Write the forward primer sequence, indicate 5' and 3' ends b. Write the reverse primer sequence, indicate 5' and 3' ends
The following chromatogram is obtained from the sequencing reaction. Using the key below, what message did you integrate into the yeast genome? AATATTAAAGTATAGAACGACTGTGAA A B с D E F G H I AAA AAT AAG AAC ATT ATG ATC AGT AGG J K L M N O P Q R Key GGG GGA GGT GGC GAA GAC GAT GTT GTA S T U V W X Y Z (SPACE) TTT TTC TTA TTG TGT TGA TGG TAA TAG
The short sequence shown below is part of a gene of interest. You decide to amplify this part of the gene by PCR. One primer has been provided by your lab supervisor as shown 3 GAGAGC 51 5'TGAGCAGCGATGCCGATGTTGCCAATGCAGCTCTCG-3'| Which of following additional primers would you need to make? OA S'ACTGCT'3 OB. S'ACGAGT3 OC. SUGAGCA'S D. STGAGCA'3
2) The partial coding sequence of the Spike protein for SARS- COV-2 is shown below: You are tasked with designing 2 primers to clone this gene by PCR amplification. What are the sequences of the two 21bp primers in the 5' to 3' orientation that will allow you successfully amplify this gene? АTGTTTGTTTTТСTTGTTTTATTGCCACTAGTCTСТAGTCAGTGTGTTAATCTTACAАССAGAACTCAАТТАСССССТGСАТАСАСТААТСTTTCАCАC GACCAGTTGCTGTAGTTGTCTCAAGGGCTGTTGTTCTTGTGGATCCTGCTGCAAATTTGATGAAGACGACTCTGAGCCAGTGCTCAAAGGAGTCAAA O A) ATGTTTGTTTTTCTTGTTTTA and GTCAAATTACATTACACATAA O B) TAAAACAAGAAAAACAAACAT and TTATGTGTAATGTAATTTGAC O C) ATGTTACTTGGTGACCAGTTG and GTCAAATTACATTACACATAA O D) ATGTTTGTTTTTCTTGTTTTA and TTATGTGTAATGTAATTTGAC
You are asked to design PCR primers (18 nucleotides) to amplify the coding region (without the stop codon) of the following gene. Please write down the sequences of primers. (Indicate the 5' and 3' of the primers). 4. 5'atgaagaccaatagagagcaggaaatttacgttgaaagaagcttcaaaccaaacaattcaacaattcagaatttgatggacattgaaag gttcattttgcctcacacttctacatcaggtgtcgcaaggctcaaaatgagggtcatatcatgggtegggcttcagttctacaactactga-3’
The target sequence for a PCR amplification is shown below. When the final cycle of PCR has ended, the resulting amplicon will be how many base pair (bp) long? 5' ACGTGCGAGCACGTATATATGTCGCGTGCTGAGTGTAGCGTATGCGAATCACCGC 3' 3' TGCACGCTCGTGCATATATACAGCGCACGACTCACATCGCATACGCTTAGTGGCG 5' 31bp 39bp 42bp 47bp None of the above
Map of pUC18 is shown on the here. Describe how to select recombinant clones if a foreign DNA is inserted in to the polylinker site of pUC18 and then introduced into E. coli cells.. Hindll Sphl Sbfl Pstl BspMI Acci Hincli Sall Xbal BamHI Aval Smal Xmal Acc651 Kpnl Banll Eco53kl Sacl Apol ECORI lacz MCS LacR binding site Plac PUC18 Amp 2686 bps PMB1 ori
The figure below depicts the start of the CDNA sequence of the gene AGAMOUS from the model plant Arabidopsis thaliana. ORIGIN 1 ctaaatgtac tgaaaagaaa caccagttta attaattata cttccctcat atataactat 61 caaccaagta caaaactttt gtcaattctc aaaatcaact ttcaccacat aattatctaa 121 catgtgtatg ttccaaaacc agtttaaata gaattacttt tcagaaaata catgtatatt 181 aactctatct aataaagaag aaacacatac ttatctcata gattccattc ataaaactat 241 gctttagtga gtaagaaaac cagtaatcaa acacaaattg acaagacact atatggatgt 301 aaaaagtggg gaaaaatggt gataaatagt agagaaaatt aaaaagaaaa aaaatattcc 361 tttataaatg tatataccca tctcttcacc agcacaacct taccttccat tttctgcaac 421 ttctccaaat ctcatacttt ccagaaaatc attttcccaa gaaaaataaa actttcccct 481 ttgttcttct ccccccaaca ggtcaggcta gtattttgtt gttccccaat cctttcaaac 541 attatcttct tcataatatt actttgcttc acttcttttg gtccttaaac caaatttttg 601 ttcttcttta gtttcctttt ttcccgtttt gatttgattc tttttatgtt atttagagag 661 aaacaagatt cacaaattct ctgatcttct ttaactcttt aaaacttttc tttttcacac 721 tctagattta aattatccct atggttacaa…
Given below is the DNA template. What are the gene products? 3’ TACCGGCCTATCTAGGGCCATGGCTTAATTCCC 5’ 5’ ATGGCCGGATAGATCCCGGTACCGAATTAAGGG3’
For this activity you will draw out the first steps of the PCR reaction, using the following as a short template. Take a picture of your drawing and upload it to this Question. 5' TTGCGTACGTGCATGTGTGCACATATGTCC 3' 3' AACGCATGCACGTACACACGTGTATACAGG 5° In your upload submission be sure to include: 1. Label the 5' and 3' end of the template and each primer, and indicate the direction in which polymerization will take place. 2. Write the sequence of a 10 base pair Forward primer and a 10 base pair Reverse primer binding to the template strand. Add an arrow showing which direction polymerization will take place 3. Add a DNA polymerase to the drawing above showing where the polymerase will bind. 3. Draw and label a DNTP nucleotide. Show how this monomer gets incorporated into a new DNA strand?

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