1)What is the natural color of cytoplasm? How is it related to our need to stain bacterial cells? 2) Which of the following is the main goal when staining a culture? A) improve contrast B) identify bacteria C) Visualize bacterial structures
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1)What is the natural color of cytoplasm? How is it related to our need to stain bacterial cells?
2) Which of the following is the main goal when staining a culture?
A) improve contrast
B) identify bacteria
C) Visualize bacterial structures
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- You accidentally switch the Crystal violet and Safranin steps during your Gram stain. When you look under the microscope, what color will a Gram positive bacteria, like Staphylococcus aureus, be? a)red/pink b)purple c)colorlessDifferentiate the staining procedures. Gram Stain Acid Fast Endospore Capsule Flagella a) Principle b) Characteristic of Bacteria viewed using this stain. (e.g. bacterial type, strain, or targeting particular bacterial feature/s) c) Stain/s Used d) Type of Stain e) Mechanism of Staining (of each stain used) f) Flow of Method1. a) What is Gram staining? b) Describe step by step the procedure c) Explain the basis of Gram staining to differentiate bacteria
- 3) The use of the Gram stain in microbiology is important because it differentiates A) Bacteria from Archaea. B) prokaryotic from eukaryotic cells. C) bacterial cells with different types of cell walls. D) archaeal cells with different types of metabolism.This part of the staining process helps the primary stain to remain in the cell during decolorization. O 1) Secondary stain O 2) primary stain O 3) counterstain O 4) fixing reagent O 5) mordantIn preparing a bacterial smear for staining, heat fixation is done after the smear dries up.a.) Give the purpose of heat fixation.b.) What can be observed in wet mounts or hanging-drop slides that cannot be observed in heat-fixed slides?
- Which of the following methods used to enumerate cells often requires specialized staining to observe nonpigmented bacteria? A) Spectrophotometry/turbidity B) Spread-plating C) Microscopy D) Spread-plating, turbidity, and microscopya. What can you observe in viewing your stained bacterial slide under the microscope if you fixed a lot of bacterial colonies in your slide during smear preparation? b. What can you observe in viewing your stained bacterial slide under the microscope if you heat fixed your slide in a much longer exposure to heat?Why do we need to stain microorganisms?
- N Please observe the following cells that have been Gram stained. "* 3 E ER $ JO 4 R % 5 6 F G & Y I U 8 00How can colony morphology be used in the identification of bacterial species? What are the environmental factors that affect the growth of bacteria? What is agar (or agar-agar)? Is this different from nutrient agar? Why? How is xylol used to clean the lens of a microscope? What may happen if xylol is constantly used for this purpose? For what purpose would you adjust each of the following microscope components during a microscopy exercise? iris diaphragm coarse adjustment knob fine adjustment knob condenser mechanical stage controlIn lab, we used the Gram stain to differentiate between Gram-positive and Gram-negative bacteria. Some bacteria stain purple (retaining the crystal violet) while others stain pink (from the counter-stain safranin). a) What is the biological molecule that is present in these bacteria that produces different results between Gram-positive and Gram-negative bacteria (be specific!) AND b) What is the difference between Gram-positive and Gram-negative bacteria that creates the different staining patterns (purple vs. pink)? (2-3 sentences max)