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2. Enzyme Kinetics at Different Temperatures During a test of kinetics of an enzyme catalyzed reaction, the following data were recorded: a. Determine the Michaelis-Menten constant and Vmax for the reaction with no inhibitor present for temperatures of 30C and 49.6C. b. Use the results from part A to indicate if the reaction is in the temperature activation region when T is increased from 30C to 49.6C. c. Create an Arrhenius plot using the Vmax results for the two temperatures in part A for an enzyme concentration of 1.6 and estimate the values of Ea (if indeed the enzyme is being activated at the two temperatures) and the pre-exponential constant. Table 2 Eo T I S V g/L °C mmol/mL mmol/mL mmol/mL-min 1.6 30 0 0.1 2.63 1.6 30 0 0.033 1.92 1.6 30 0 0.02 1.47 1.6 30 0 0.01 0.96 1.6 30 0 0.005 0.56 1.6 49.6 0 0.1 5.13 1.6 49.6 0 0.033 3.70 1.6 49.6 0 0.01 1.89 1.6 49.6 0 0.0067 1.43 1.6 49.6 0 0.005 1.11

2. Enzyme Kinetics at Different Temperatures During a test of kinetics of an enzyme catalyzed reaction, the following data were recorded: a. Determine the Michaelis-Menten constant and Vmax for the reaction with no inhibitor present for temperatures of 30C and 49.6C. b. Use the results from part A to indicate if the reaction is in the temperature activation region when T is increased from 30C to 49.6C. c. Create an Arrhenius plot using the Vmax results for the two temperatures in part A for an enzyme concentration of 1.6 and estimate the values of Ea (if indeed the enzyme is being activated at the two temperatures) and the pre-exponential constant. Table 2 Eo T I S V g/L °C mmol/mL mmol/mL mmol/mL-min 1.6 30 0 0.1 2.63 1.6 30 0 0.033 1.92 1.6 30 0 0.02 1.47 1.6 30 0 0.01 0.96 1.6 30 0 0.005 0.56 1.6 49.6 0 0.1 5.13 1.6 49.6 0 0.033 3.70 1.6 49.6 0 0.01 1.89 1.6 49.6 0 0.0067 1.43 1.6 49.6 0 0.005 1.11

Introduction to Chemical Engineering Thermodynamics
Introduction to Chemical Engineering Thermodynamics
8th Edition
ISBN:9781259696527
Author:J.M. Smith Termodinamica en ingenieria quimica, Hendrick C Van Ness, Michael Abbott, Mark Swihart
Publisher:J.M. Smith Termodinamica en ingenieria quimica, Hendrick C Van Ness, Michael Abbott, Mark Swihart
Chapter1: Introduction
Section: Chapter Questions
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2. Enzyme Kinetics at Different Temperatures During a test of kinetics of an enzyme catalyzed reaction, the following data were recorded: a. Determine the Michaelis-Menten constant and Vmax for the reaction with no inhibitor present for temperatures of 30C and 49.6C. b. Use the results from part A to indicate if the reaction is in the temperature activation region when T is increased from 30C to 49.6C. c. Create an Arrhenius plot using the Vmax results for the two temperatures in part A for an enzyme concentration of 1.6 and estimate the values of Ea (if indeed the enzyme is being activated at the two temperatures) and the pre-exponential constant. Table 2 Eo T I S V g/L °C mmol/mL mmol/mL mmol/mL-min 1.6 30 0.1 2.63 1.6 30 0 0.033 1.92 1.6 30 0 0.02 1.47 1.6 30 0 0.01 0.96 1.6 30 0 0.005 0.56 1.6 49.6 0 0.1 5.13 1.6 49.6 0 0.033 3.70 1.6 49.6 0 0.01 1.89 1.6 49.6 0.0067 1.43 1.6 49.6 0 0.005 1.11
Transcribed Image Text:2. Enzyme Kinetics at Different Temperatures During a test of kinetics of an enzyme catalyzed reaction, the following data were recorded: a. Determine the Michaelis-Menten constant and Vmax for the reaction with no inhibitor present for temperatures of 30C and 49.6C. b. Use the results from part A to indicate if the reaction is in the temperature activation region when T is increased from 30C to 49.6C. c. Create an Arrhenius plot using the Vmax results for the two temperatures in part A for an enzyme concentration of 1.6 and estimate the values of Ea (if indeed the enzyme is being activated at the two temperatures) and the pre-exponential constant. Table 2 Eo T I S V g/L °C mmol/mL mmol/mL mmol/mL-min 1.6 30 0.1 2.63 1.6 30 0 0.033 1.92 1.6 30 0 0.02 1.47 1.6 30 0 0.01 0.96 1.6 30 0 0.005 0.56 1.6 49.6 0 0.1 5.13 1.6 49.6 0 0.033 3.70 1.6 49.6 0 0.01 1.89 1.6 49.6 0.0067 1.43 1.6 49.6 0 0.005 1.11
2. D A) VMAX KM T= T= 30C 3.2948 0.0244 49.6 C 6.3411 0.0235 B) Yes, it is in the Activation Temp Area when T = 30C - 49.6C C). V = (Ae-(A) * CE) | EA = -49.703 R Plot
Transcribed Image Text:2. D A) VMAX KM T= T= 30C 3.2948 0.0244 49.6 C 6.3411 0.0235 B) Yes, it is in the Activation Temp Area when T = 30C - 49.6C C). V = (Ae-(A) * CE) | EA = -49.703 R Plot
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