2. The attached figure is a titration curve for the amino acid glycine. At which pH is glycine expected to have its best buffering capacity? a) 2.1-3.1 b) 7.3-7.4 c) 6.8-7.4 d) 5.97 e) 12 Ans: a) 2.1 3.1
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- What is the pH of a 0.1 M phosphate buffer (pKa = 6.86) that contains 10 times more of the conjugate acid form than the conjugate base form? a.) 5.86 b.) 16.86 c.) -3.14 d.) 6.8651. The OH concentration of a solution is 4.3 × 10-¹0 M. What is the pH? A) -9.37 B) 9.37 C) -4.63 D) 4.63 E) 13.4 52. If the K₂ of an acid is 1.38 x 10-7, what is the pk₂? A) 6.86 B) 7.14 C) 8.68 D) 10.7 E) 1.38 53. The conjugate base of H₂PO4-¹ is: A) H₂PO4 B) HIPO C) HPO D) HPO. E) POConsider a HCI solution with a pH of 4.70. a) Calculate the concentration of HCl. b) Identify the two underlying assumptions in part a). c) The original solution is diluted by 1,000 fold. Calculate the pH of the resulting solution. Make sure to indicate if the forementioned assumptions are still valid.
- Select all that apply, meaning there can be more than one correct answer Common factors affecting menbrane fluidity include a.) Temperature. b.) presence of cholesterol c.) number of double bonds in the fatty acid chains. d.) length of phospholipid fatty acid chains Which of the following methods can seperate proteins based on their mass? a.) centrifugation b.) gel filtration chromatography c.) SDS polyacrylamide gel electrophoresis d.) ion exhange chromatographyDetermine the weight/volume of the chemicals needed to prepare the following solutions: a) 100 ml of 0.9% (w/v) saline (NaCl) b) 30 ml of 50% glycerol (v/v) c) Electrophoresis requires TAE, which is a specific mixture of Tris base, acetic acid, and EDTA. TAE is normally made as a 50X concentrated stock. Provide a recipe to make 40 ml of 50X TAE. The recipe for one liter of 50X TAE is as follows: 242g Tris base, 57.1 ml glacial acetic acid, 100 ml 0.5 M EDTAthe pH of a solution is decreased from 9 to 8, it means that the A) concentration of H+ has decreased to one-tenth (1/10) what it was at pH 9. B) concentration of H+ has increased 10-fold (10X) compared to what it was at C) concentration of OH- has increased 10-fold (10X) compared to what it was at D) concentration of OH- has decreased to one-tenth (1/10) what it was at pH 9. E) Both B and D are correct. p
- What volume of water is needed to make 40uL of 100ug/mL BSA from a 2mg/mL BSA stock? Group of answer choices A) 0.8uL B) 38uL C) 5uL D) 2uL E) 2000uLa) A bottle is labeled Demerol (meperidine) 50 mg/cc. How many cubic centimeters are needed to give a 125 mg dose? b) Promazine HCL comes as 500 mg/10 ml. How many milliliters are needed to give 150 mg dose? c) Hyaluronidase comes as 150 U/cc. How many cubic centimeters for a 30 U dose?75 mL of 0.2 M NaOH were added to 100 mL of 0.1M aspartate solution, pH=pI. What is the new pH? pKavalues for aspartate are 1.88, 3.65 (R-group) and 9.60. 8. A) 1.40 B) 4.13 C) 6.62 D) 2.76 E) 9.60 If we are not given the starting pH, how do we do this question?
- 5. We have 100 mL of 5X TBE (Tris/Borate/EDTA) solution and need to make 1 L of 2X solution. Solid compounds also exist for each of these materials. The molecular weight of Tris is 157.60; Borate has molecular weight of 58.80 and EDTA has a molecular weight of 292.44. a) What is the stock concentration? b) What is the final concentration? c) What is the starting volume? d) What is the final volume? e) Write out the equation used to solve this. Show all your work. Are we able to make a solution using this dilution method using are starting materials? If not, propose another way to make the desired concentration and volume of the solution. Show your work.In a hospital laboratory, a 10.0 mL sample of gastric juice, obtained several hours after a meal, was titrated with 0.01 M NaOH to neutral pH of 7.0; 72.0 mL of NaOH was required for this titration. The patient’s stomach contained no ingested food or drink, thus assume that no buffers were present. What was the pH of the 10.0 mL gastric juice sample before titrating with NaOH?Define the following terms: a. lactone b. aldaric acid c. aldonic acid d. uronic acid e. epimerization