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- 2. Which of the following statements is true regarding steam sterilization? a. It is a universal technique, applicable to a product of any nature. b. At the same temperature, usually more effective than dry heat. c. Steam sterilization is amenable to a continuous workflow. d. The purpose of the steam is to keep the product from drying out during sterilization. Steam cannot be combined any other sterilization technique. е.1. Which of the following methods is the best way to sterilize the apparatus used in a surgical operation? A. Pasteurization B. Incineration C. Boiling in water D. Autoclaving E. Disinfect with 75% alcohol1. Give the principle involved in the operation of the following instruments and their specific purposes: a. Autoclave b. Hot air Sterilizer c. Inspissator
- 1. Given the following common laboratory materials, which of the three methods of heat sterilization do you think is the most appropriate and practical to use in sterilizing them Why do you think so ? Method of heat sterilization Reason a. Empty flasks b. L-rod (glass rod) c. 10 ml glass pipet d. Wire needles e. Antibiotic solution1. Which of the following is NOT true of spread plating method? A. the maximum inoculum allowed is 1.0 ml B. spreading of the inoculum is done using a disinfected glass L-rod C. A pre-solidified medium is required prior to inoculation of the sample. D. A and B 2. Plates inoculated with bacteria are inverted during incubation to: A. prevent contamination B. limit the colony growth C. A and B D. A and C E. All of the above F. None of the above3. Describe the following inexpensive measures of treating drinking water: A. Boiling B. Chlorination C. Solar water disinfection (SODIS) 4. Why is boiling water not a recommended sterilization method? 5. List examples of transfusion-transmitted infections. How are these infections prevented?
- 2. What of the following types of culture media allows the growth of specific microorganisms and suppresses the growth of other microorganisms? A. Selective B. Non-selective C. Enriched D. Differential E. None of the above1-The method of sterilizations we used in the tissue culture lab a. Fumigation b. Filtration C. Autoclave d. Sun light1. Describe and give the principle of each equipment used for sterilization in microbiology. (refer to equipment drawn only)
- 1. TRUE or FALSE. An autoclave can be used to sterilize growth media?The following methods used for sterilizing an antibiotic solution. i. Dry heat sterilization ii. Microfiltration iii. Autoclaving iv. Desiccation Select one: O a. i and ii O b. i and iii O c. ii and iv O d. ii onlyNeutralization of residual disinfectant could be made by: a. Dilution if the concentration exponent is high b. Dilution if the concentration exponent is low c. Adding specific agent e.g. 3% Tween 80 d. Either a or c e. Either b or c
