4. A polypeptide comprised of 17 amino acid res- idues with the sequence on the right is ob-served by spectroscopic methods to undergo transitions from an a-helix conformation to a B-sheet as a function of pH and concentration. The acetyl and carboxamide groups are attached covalently to deri- vatize the N- and C-terminal residues, respectively, to avoid charge effects of the end CH3CO-ETATKAELLAKYEATHK-CONH2 groups. (a) (: amino acid residues. Write the sequence of this polypeptide with corresponding 3-letter abbreviations for the (b) At pH < 4 the polypeptide forms an a-helix. (i) ( . ) What is the direction of the macrodipole of the helix? and (ii) (: with respect to the positive and negative ends of the macrodipole? Are the charges on the N- and C-terminal residues stabilizing or de-stabilizing (c) ( stabilize the helical conformation. What are the most likely protonation states of the side-chains that provide a-helix stabilizing interactions? Use the helical wheel (at the back of the problem set) to identify side chain interactions that
4. A polypeptide comprised of 17 amino acid res- idues with the sequence on the right is ob-served by spectroscopic methods to undergo transitions from an a-helix conformation to a B-sheet as a function of pH and concentration. The acetyl and carboxamide groups are attached covalently to deri- vatize the N- and C-terminal residues, respectively, to avoid charge effects of the end CH3CO-ETATKAELLAKYEATHK-CONH2 groups. (a) (: amino acid residues. Write the sequence of this polypeptide with corresponding 3-letter abbreviations for the (b) At pH < 4 the polypeptide forms an a-helix. (i) ( . ) What is the direction of the macrodipole of the helix? and (ii) (: with respect to the positive and negative ends of the macrodipole? Are the charges on the N- and C-terminal residues stabilizing or de-stabilizing (c) ( stabilize the helical conformation. What are the most likely protonation states of the side-chains that provide a-helix stabilizing interactions? Use the helical wheel (at the back of the problem set) to identify side chain interactions that
Biology 2e
2nd Edition
ISBN:9781947172517
Author:Matthew Douglas, Jung Choi, Mary Ann Clark
Publisher:Matthew Douglas, Jung Choi, Mary Ann Clark
Chapter3: Biological Macromolecules
Section: Chapter Questions
Problem 16RQ: The helix and the pleated sheet are part of which protein structure? primary secondary tertiary...
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