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- COSY is one of 2D spectras which are widely used for the structure determination of proteins with a mass of up to 10 kD. What gives rise to cross signals in a COSY experiment?Calculate the relative population differences (Nɑ - Nβ)/N for 13C nuclei in fields of (i) 0.50 T, (ii) 2.5 T, and (iii) 15.5 T at 25 °C.in the determination of Keq in spectrophotometry, why is it that [KSCN] is lower in the preparation of unknown solutions.
- (2) Adenine has a molar absorbance of 4 13.1 M-1cm-1 for 263 nm nucleic acid. What is the molar concentration of an acid solution that shows a permeability of 75% when 2 ml of it is placed in an absorption cell with a thickness of 1 cm?Based on the chromatograms, Symmetry C18 (top) is less selective than Symmetry Shield RP18 (bottom) for the analytes given, true or false?Q4.2 Sensitivity If 13C data were collected at this field using a protein concentration of 500 µM what is the effective protein concentration that gives rise to the measured NMR signal? Enter your answer here
- Assuming an integrated luminosity of 100 fb is collected at the ATLAS detector at the LHC, calculate the number of h ZZ events where both Z bosons decay to either a ete pair or a u u palr. You may assume that the total Higgs (h) production cross section is o, = 2 x 10' fb, the branching fraction for a Z boson decay to a ee pair is 3.36%, the branching fraction for a Z boson decay to a "u pair is 3.36%, and the branching fraction for a Higgs boson to decay to a pair of Z bosons is 2.7%. Enter your answer in the box below rounded to the nearest integer (e.g. 1234.4 should be entered as 1234). AncwerBy what factor must the applied magnetic field be increased for the relative population difference (Nɑ - Nβ)/N to be increased by a factor of 5 for (i) 1H nuclei, (ii) 13C nuclei?50 uL of an aqueous sample of double-stranded DNA is dissolved in 950uL of water. This diluted solution has a maximal absorbance of 0.326 at 260 nm. What isthe concentration of the original (more concentrated) DNA sample, expressed in ug/ul? I would like to know the process of this question. And I was wondering as well if the total diluted volume would be 950ul + 50ul = 1000ul
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