5-azocytidine is an inhibitor of DNA methyltransferases. If this compound was fed to pregnant female mouse, how would it affect the coat color of offspring carrying the agouti Avy allele? Explain your answer
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5-azocytidine is an inhibitor of DNA methyltransferases. If this compound was fed to pregnant
female mouse, how would it affect the coat color of offspring carrying the agouti Avy allele?
Explain your answer
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- 5-Azocytidine is an inhibitor of DNA methyltransferase. If thisdrug were fed to female mice during pregnancy, explain how youthink it would affect the coat color of offspring carrying the Avyallele.Antibiotics such as chloramphenicol, tetracycline, and erythromycin inhibit protein synthesis in bacteria, but have no effect on the synthesis of proteins encoded by eukaryotic nuclear genes. Cycloheximide inhibits the synthesis of proteins encoded by nuclear genes, but has no effect on bacterial protein synthesis. How might these compounds be used to determine which proteins are encoded by mitochondrial and chloroplast genomes?How can one most effectively silence the myofilament gene unc-22 in C. elegans to produce the twitching phenotype? O A. Screen thousands of strains for a spontaneous unc-22 mutation O B. Soak worms in mutagenic solutions of EMS and screen the progeny OC. Inject antisense strands of unc-22 RNA into worm ovaries, which will be taken up by germ line cells and expressed in progeny O D. Inject a high dose of unc-22 MRNA into worm ovaries, which will be taken up by germ line cells and expressed in progeny O E. Culture worms with E. coli transformed with a plasmid that codes for a dsRNA unc-22 construct
- In a haploid yeast strain, eight recessive mutationswere found that resulted in a requirement for theamino acid lysine. All the mutations were found to revert at a frequency of about 1 × 10−6 except mutations5 and 6, which did not revert. Matings were madebetween a and α cells carrying these mutations. Theability of the resultant diploid strains to grow onminimal medium in the absence of lysine is shown inthe following chart (+ means growth and − means nogrowth.)1 2 3 4 5 6 7 81 − + + + + − + −2 + − + + + + + +3 + + − − − − − +4 + + − − − − − +5 + + − − − − − +6 − + − − − − − −7 + + − − − − − +8 − + + + + − + −a. How many complementation groups were revealedby these data? Which point mutations are foundwithin which complementation groups?The same diploid strains are now induced to undergosporulation. The vast majority of resultant spores areauxotrophic; that is, they cannot form colonies whenplated on minimal medium (without lysine). However,particular diploids can produce rare spores…What phenotypes do you think a homozygous tra1hsn animal with a gain of function mutation in the Egl1 would have.Map distances were determined for four differentgenes (MAT, HIS4, THR4, and LEU2) on chromosome III of the yeast Saccharomyces cerevisiae:HIS4 ↔ MAT 37 cMTHR4 ↔ LEU2 35 cMLEU2 ↔ HIS4 23 cMMAT ↔ LEU2 16 cMMAT ↔ THR4 20 cMWhat is the order of genes on the chromosome?
- What phenotypes do you think a homozygous tra1hsn animal with a loss of function Egl-1 mutation would have.Based on the sensitivity of DNA to DNase I, as illustrated in Figure , which type of chicken hemoglobin (embryonic or adult) is likely to be produced in the highest quantity in the following tissues and developmental stages? Q. Brain cells throughout developmentThe rosy (ry) gene of Drosophila encodes an enzymecalled xanthine dehydrogenase. Flies homozygous forry mutations exhibit a rosy eye color. Heterozygousfemales were made that had ry41 Sb on one homologand Ly ry564 on the other homolog, where ry41 andry564 are two independently isolated alleles of ry. Ly[Lyra (narrow) wings] and Sb [Stubble (short) bristles] are dominant mutant alleles of genes to the left and right of ry, respectively. These females arenow mated to males homozygous for ry41. Out of100,000 progeny, 8 have wild-type eyes, Lyra wings, andStubble bristles, while the remainder have rosy eyes.a. What is the order of these two ry mutations relativeto the flanking genes Ly and Sb?
- Gene A, which encodes alcohol dehydrogenase, is labeled with a red fluorophore in a FISH experiment with one chromosome of a homologous pair, with results shown below. Gene B is labeled in green and encodes Notch2NL, a gene which may help to explain brain size differences between humans and chimpanzees, and which with increased dosage, may lead to a condition referred to as macrocephaly (enlarged head circumference sometimes associated with learning disabilities). What can you infer about these two genes given the results seen below?Deletions in bacterial chromosomes give the following data: Region of deletion Al Gene A activity +++ A2 АЗ A4 AS +++ (i) Where is the gene located? Explain your answer. Name the scientific term that describes the appearance of a recessive phenotype due to deletion of dominant gene. (ii) (iii) The phenotypic consequences of deletion depend on two factors. What are they? Give one example of human genetic disorder caused by chromosomal deletion by indicating the region of deletion in chromosome. (iv)Explain why loss-of-function hedgehog and smoothened mutations yield the same phenotype in flies, but a loss-of- function patched mutation yields the opposite phenotype.