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STEM WOrkplace Practices Q5
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- 7. Which of the following can be achieved using dialysis? I. Desalting II. Concentration III. Buffer exchange IV. Cell harvesting A. I and III B. All except II C. All except IV D. All of the above7. In collecting urine samples for testing, it is preferable to obtain a “mid-stream catch" or a "clean catch"; that is, to only collect urine after the flow has started. Why is this necessary?4. What happens when urine is allowed to stand for sometime?
- 3. A mixture of proteins will be separated on a gel filtration column. The first peak elutes in 12 ml from the column, and the last peak elutes in 42 ml. The flow rate in the column is 1.5 ml/minute. The column diameter is 1.0 cm, and the height is 60 cm. Protein MW Кр NaCl 58.44 1 aprotinin 6500 0.69 43,000 75,000 2,000,000 ovalbumin 0.43 Conalbumin 0.35 Blue Dextran a. Calculate the elution time of each protein. b. Explain the assumptions that can be used to scale up the column to a flow rate of 200 ml/minute. c. Calculate the dimensions of the new column. ||3- What is the purpose of first filtration and second filtration?1. How do you calculate the initial concentration of a sample? 2. Indicate whether you think that the number of cfu/ ml calculated would be too high or too low if you make this mistake. ~You add 9 ml of broth (water) to each tube. You add 1 ml of culture to the first tube and mix. You get distracted, and transfer 1ml of the dilutant (mixture of culture and broth) to the third tube instead of the second. You perform the rest of the series as described. High or LOW ?
- 9. Explain the purpose of evaluating the fractional excretion (FE) test of electrolytes. What should be considered when collecting urine samples?6. A quantitative test for potassium is performed on a 24- hour urine specimen. The total volume of urine collected is 2400 mL. The potassium concentration in the urine specimen is 800 mEq/L. (MW of potassium = 39 g). Express the potassium concentration in: a. mg/24 hr b. ppm3. In a clinical setting, urine is often examined under a microscope. What information about a patient's health might be gained through this type of examination?
- 3. A urine protein value of 75mg/dl from a 12 hour urine sample with a volume of 2100mL. Calculate the urine protein value reported as grams/12 hours. Round off to the nearest hundredth1. In a urinalysis, what does the test of PH reflect a. The presence of protein in the urineb. the presence of glucosec. the possibility of kidney stones1. True or False: Cations and anions both easily cross the filtration membrane in a nephron 2. What type of pressure is present in a renal capsule? a.Hydrostatic Pressure b. Colloid osmotic pressure c. Both a and b are correct 3. Which activity occurs first in a nephron? a. filtration b. reabsorption c. secretion 4. True or False: During blood filtration, water moves into the nephron. 5. What cell type helps to form a filtration membrane? a. hepatocyte b.podocyte c.Distal convoluted tubule
