Homework Help is Here – Start Your Trial Now!
Science
Biology
A B cat frame-shift no cat no cat cat nonsense cat C % survival 120 100 80 60 40 20 0 42 cat cats cats 37 30 cats 23 [°C]

A B cat frame-shift no cat no cat cat nonsense cat C % survival 120 100 80 60 40 20 0 42 cat cats cats 37 30 cats 23 [°C]

Human Heredity: Principles and Issues (MindTap Course List)
Human Heredity: Principles and Issues (MindTap Course List)
11th Edition
ISBN:9781305251052
Author:Michael Cummings
Publisher:Michael Cummings
Chapter13: An Introduction To Genetic Technology
Section: Chapter Questions
Problem 20QP: Analyzing Cloned Sequences A base change (A to T) is the mutational event that created the mutant...
See similar textbooks
icon
Related questions
Question

Explain the technique used in the figure of each panel and what the data shows for each. 

  Panel Technique These data show:
Figure 4

A

B

c

    
Figure 1 Figure 2 Figure 3 Figure 4 Figure 5 Panel A B C A B C D E A B C D A B C Technique figure 1A-fluorescent microscopy was used These data show: figure 1A-low level GFP protein expression was observed in B. subtilis mutants with frameshift and nonsense mutations in the GFP gene
Transcribed Image Text:Figure 1 Figure 2 Figure 3 Figure 4 Figure 5 Panel A B C A B C D E A B C D A B C Technique figure 1A-fluorescent microscopy was used These data show: figure 1A-low level GFP protein expression was observed in B. subtilis mutants with frameshift and nonsense mutations in the GFP gene
A B cat frame-shift no cat no cat cat nonsense cat C 120 100 80 60 40 20 % survival 0 42 catt cats cats 37 30 cats 23 [°C] Fig. 4. Errors in gene expression enable survival of strains carrying a mutated antibiotic resistant gene. (A) Strains PY79 (no cat), SB463 (cat), SB477 (catfs), and SB475 (catns) were grown on 4-µg/mL chloramphenicol-containing plates. Plates were imaged after 40 h of incubation at 30 °C. (B) Strains PY79 (no cat), SB463 (cat), SB477 (catf), and SB475 (catns) were streaked on 10-µg/mL chloramphenicol- containing plates and imaged after 40 h of incubation at 30 °C. (C) The viability (%) of SB477 (catfs) (red bars) and SB475 (catns) (blue bars) at different temperatures when plated on chloramphenicol-containing plates. Cells were grown in CH medium at the different temperatures in the absence of chloramphenicol, and plated at mid-exponential phase on either LB or LB supplemented with 4-µg/mL chloramphenicol plates. Percent-viability is the number of colonies growing on chloramphenicol-containing plates relative to the total number of colonies growing on LB plates incubated at the equivalent temperature (Materials and Methods). PY79 (no cat) strain did not grow on chloramphenicol-containing plates, whereas SB463 (cat) showed ~100% viability at any growth temperature. The viability on LB plates of all tested strains was similar, with colony number ranging from 350 to 500.
Transcribed Image Text:A B cat frame-shift no cat no cat cat nonsense cat C 120 100 80 60 40 20 % survival 0 42 catt cats cats 37 30 cats 23 [°C] Fig. 4. Errors in gene expression enable survival of strains carrying a mutated antibiotic resistant gene. (A) Strains PY79 (no cat), SB463 (cat), SB477 (catfs), and SB475 (catns) were grown on 4-µg/mL chloramphenicol-containing plates. Plates were imaged after 40 h of incubation at 30 °C. (B) Strains PY79 (no cat), SB463 (cat), SB477 (catf), and SB475 (catns) were streaked on 10-µg/mL chloramphenicol- containing plates and imaged after 40 h of incubation at 30 °C. (C) The viability (%) of SB477 (catfs) (red bars) and SB475 (catns) (blue bars) at different temperatures when plated on chloramphenicol-containing plates. Cells were grown in CH medium at the different temperatures in the absence of chloramphenicol, and plated at mid-exponential phase on either LB or LB supplemented with 4-µg/mL chloramphenicol plates. Percent-viability is the number of colonies growing on chloramphenicol-containing plates relative to the total number of colonies growing on LB plates incubated at the equivalent temperature (Materials and Methods). PY79 (no cat) strain did not grow on chloramphenicol-containing plates, whereas SB463 (cat) showed ~100% viability at any growth temperature. The viability on LB plates of all tested strains was similar, with colony number ranging from 350 to 500.
Expert Solution
trending now

Trending now

This is a popular solution!

steps

Step by step

Solved in 3 steps

SEE SOLUTIONCheck out a sample Q&A here
Blurred answer
Similar questions
  • SEE MORE QUESTIONS
Recommended textbooks for you
Human Heredity: Principles and Issues (MindTap Co…
Human Heredity: Principles and Issues (MindTap Co…
Biology
ISBN:
9781305251052
Author:
Michael Cummings
Publisher:
Cengage Learning
Intro To Health Care
Intro To Health Care
Health & Nutrition
ISBN:
9781337338295
Author:
Mitchell
Publisher:
Cengage
SEE MORE TEXTBOOKS