A biochemist discovers and purifies a new enzyme, generating the purification table shown. From the information given, complete the table column showing the specific activity of the enzyme after each purification procedure. Total protein Total activity Specific activity Procedure (mg) (units) (units mg-1) Crude extract 10, 000 68, 000 Precipitation (salt) 5, 000 65, 000 Precipitation (pH) 4, 000 56, 000 Ion-exchange chromatography 70 49, 000 Affinity chromatography 12 42, 000 Size-exclusion chromatography 8 40, 000

A biochemist discovers and purifies a new enzyme, generating the purification table shown. From the information given, complete the table column showing the specific activity of the enzyme after each purification procedure. Total protein Total activity Specific activity Procedure (mg) (units) (units mg-1) Crude extract 10, 000 68, 000 Precipitation (salt) 5, 000 65, 000 Precipitation (pH) 4, 000 56, 000 Ion-exchange chromatography 70 49, 000 Affinity chromatography 12 42, 000 Size-exclusion chromatography 8 40, 000

Biochemistry

9th Edition

ISBN:9781319114671

Author:Lubert Stryer, Jeremy M. Berg, John L. Tymoczko, Gregory J. Gatto Jr.

Publisher:Lubert Stryer, Jeremy M. Berg, John L. Tymoczko, Gregory J. Gatto Jr.

Chapter1: Biochemistry: An Evolving Science

Section: Chapter Questions

Problem 1P

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A biochemist discovers and purifies a new enzyme, generating the purification table below. Procedure Total protein (mg) 20,000 5,000 4,000 Activity (units) 4,000,000 3,000,000 1,000,000 800,000 750,000 675,000 1. Crude extract 2. Precipitation (salt) 3. Precipitation (pH) 4. Ion-exchange chromatography 5. Affinity chromatography 6. Size-exclusion chromatography From the information given in the table, calculate the specific activity of the enzyme after each purification procedure. b. Which of the purification procedures used for this enzyme is the most effective (I.e. gives the greatest relative increase in purity)? Which of the purification procedures is least effective? d. Is there any indication based on the results calculated in part A that the enzyme after step 6 is now pure? What else could be done to estimate the purity of the enzyme preparation? 200 50 45 a. C.
A biochemist discovers and purifies a new enzyme, generating the purification table below. Procedure Total protein (mg)Activity (units) 1. Crude extract 20,000 4,000,000 2. Precipitation (salt) 5,000 3,000,000 3. Precipitation (pH) 4,000 1,000,000 4. lon-exchange chromatography 200 800,000 5. Affinity chromatography 50 750,000 6. Size-exclusion chromatography 45 675,000 (a) From the information given in the table, calculate the specific activity of the enzyme after each purification procedure.
A biochemist discovers and purifies a new enzyme, generating the purification table below. Procedure Total Protein (Mg) Activity (Units) Crude Extract 20,000 4,000,000 Precipitation (Salt) 5,000 3,000,000 Precipitation (pH) 4,000 1,000,000 Ion Exchange Chromatography 200 800,000 Affinity Chromatography 50 750,000 Size-exclusion Chromatography 45 675,000 a) From the information given in the table, calculate the specific activity of the enzymeafter each purification procedure.b) Which of the purification procedures used for this enzyme is most effective (i.e., givesthe greatest relative…
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