A capillary tube of whole blood is collected and centrifuged to obtain a plasma sample. A 1 in 4 dilution of the plasma is made and analysed using a cholesterol assay like the one you performed in the lab. 10 μL of both the diluted plasma sample and a cholesterol standard are assayed using 1 mL of cholesterol reagent. Following incubation at 37 °C the samples are measured at 500 nm. The experiment yields the following data: A500 standard: 0.22 A500 sample: 0.59 Concentration standard: 3.5 mM What is the concentration of total cholesterol in the diltuted plasma (only type in your numerical value in the units mM to 2 decimal places)?

A capillary tube of whole blood is collected and centrifuged to obtain a plasma sample. A 1 in 4 dilution of the plasma is made and analysed using a cholesterol assay like the one you performed in the lab. 10 μL of both the diluted plasma sample and a cholesterol standard are assayed using 1 mL of cholesterol reagent. Following incubation at 37 °C the samples are measured at 500 nm. The experiment yields the following data: A500 standard: 0.22 A500 sample: 0.59 Concentration standard: 3.5 mM What is the concentration of total cholesterol in the diltuted plasma (only type in your numerical value in the units mM to 2 decimal places)?