A microorganism was streaked onto 4 nutrient agar plates and the plates were incubated at different temperatures for 24 hours. The results are shown in the picture below. Which of the following statements best describes the proteins in the organism? (the same organism was streaked on all four plates) 4°C 1127 64°C
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- The ingredients in the MR/VP Broth are: Peptone (protein) 7g Glucose 5g Phosphate buffer 5g Distilled water 1L Examine the above figure. This figure shows how the pH in the Methyl Red and Voges-Proskauer (MR-VP) broth changes during incubation from time zero to 48 hours with a VP-positive bacteria species. VP-positive bacteria initially produce lactic acid and temporarily lower the pH, but the lowered pH activates the 2,3-butanediol fermentation pathway. Accumulation of its neutral end products such as acetoin and 2,3-butanediol raises the pH by the end of the incubation time. The acetoin that accumulates in the broth also reacts with the reagents added after incubation to produce a red color. If the reagents were added and the results were recorded early, at 24 hours rather than at the full incubation time at 48 hours, would the color result for the VP (+) bacteria be the same at 24 hours than at 48 hours? If different, explain how the color result is different. What chemical…Which of the the following results of the extracellular enzyme tests is FALSE? Question 3 options: A Liquid gelatin positive for gelatinase B Clear area around bacterial growth on a starch plate after iodine is added means amylase is present C Clear zone around bacterial growth on a milk plate means caseinase is present D Solid gelatin after is positive for gelatinase E No clear zone around bacterial growth on milk plate means absence of casienaseIf the temperature of the incubator were to be increased from 35 to 45°C, how would this affect the bacterial growth curve of E. coli? (optimum temperature 20o-40oC; most human pathogens)
- A microbiologist has isolated a new strain of bacteria, and is trying to characterize aspects of its growth. Three flasks of the same media have different amounts of NaCl added. Other growth conditions are identical, and the flasks inoculated at the same time. At regular intervals over a 3 hour period, culture samples are taken and turbidity is measured. Here are the absorbance values over time in a table and graphed. Absorbance (OD600) Time (min) 20% NaCl 3% NaCl 0% NaCl 0 0.05 0.05 0.05 30 0.06 0.05 0.05 60 0.075 0.06 0.05 90 0.11 0.07 0.049 120 0.25 0.1 0.048 150 0.5 0.105 0.055 180 0.7 0.11 0.055 210 0.99 0.15 0.06 240 1.22 0.18 0.055 270 1.35 0.19 0.06 300 1.5 0.2 0.06 330 1.6 0.25 0.062 360 1.7 0.3 0.06 Based on these data, which of the following can be determined about this bacterium? It is psychrophilic. It…MacConkey agar Lactose fermenting Non-lactose fermenting colonies (lactose-) colonies (lactose*) Figure-2: MacConkey agar with-possible-outcomes| MacConkey agar... (check all that apply) can only grow bacteria that ferments lactose can only grow bacteria that produces lactose can only grow Gram positive bacteria can distinguish between bacteria that can ferment lactose can only grow Gram negative bacteriaTSA plates of microorganisms grown at 30°C or 37°C In words, describe the differences you see for each organism (E. coli M.,luteus ,S. marcescens and S. saprophyticus) grown at 30°C or 37°C (ie, amount of growth, color, etc
- Helicobacter pylori, a bacterium that causes stomach ulcers, can grow in medium with a pH above 5. It can survive but not grow in pH 2 medium as long as the medium contains Urea. You inoculate a pH 2 broth with 1,000,000 H. pylori and for 8 hours the organism did not grow. The next morning however, the medium has become quite turbid, suggesting that the organism has grown. Explain how this could happen. What phylum and class does this organism belong?Below are the results of our disc diffusion study using antiseptics and disinfectants. Use the results to answer the following questions: Lab Lab Lysol 1:10 diluted Disinfectant Isopropanol soap Tincture bleach of iodine ISP T of I BL DIS SO LYS Diameter in mm of zone of inhibition E.coli 40 mm 20 mm 10 mm 15 60 mm Bacillus 50 mm 35 mm 15 mm 8 70 mm cereus 20You have are diluting down a concentrated stock of bacteria in order to grow them. Starting with 2.1 x 105 cells/mL, you will dilute down to 1 x 103 cells/mL. How much of the bacteria will you use to make 100mL of diluted bacteria. We dilute these bacteria with a growth medium called ‘broth’ (its like Gatorade for the cells). How much broth?
- Here is an image of a UV-irradiation (wavelength 254nm) experiment performed on a plate of E. coli after incubation. Which side of the plate do you think has a 'zero-minute' negative control? A or B or neither? Explain with proper reasoning. ICA Exoli Eoli kyIn the table below designate growth (G) or no growth (NG) for each organism. Organism TSA Plates Aerobic Plate Anaerobic Plate Escherichia coli Pseudomonas aeruginosa Clostridium sporogenes (arowtn NO Grawtn 2. How would the following organisms be classified in terms of aerotolerance (from your results)? Explain how the results you recorded above support the classification. E. coli ESCnericnia coli is a FacuItative anacrobe bicause it canaoin the presence or awsine of 02 P. oeruginosa Psudomonas aeruginosa is a obigatn aoloe because It Piquires Oz For groNth C sporogenes CI 0STVI dium sporogenes is a obligare anaeobe blcauss it in the presence oF O Cannot arow 3. What region of the TSA tube is anaerobic? Aerobic? What part of the FTM tube is anaerobic? How do you know? 4. What is used to make the Anaerobic chamber anaerobic? PrOVides an anaurobIc environment oy Usin Onaunbe gas p0CK produces H, and cOzgases,and has sidi poUcn Ith metai CataNST (pall 5. What is the purpose of the dye…Bacteria grow in a nutrient solution at a rate proportional to the amount present. Initially, there are 250strands of the bacteria in the solution which grows to 800 strands after seven hours. Show (a) anexpression for the approximate number of strands in the culture at any time t and (b) the time needed forthe bacteria to grow to 1600 strands.