A mixture of tert-butylbenzene and acetophenone is adsorbed onto a silica gel chromatography column and eluted with a moderately polar solvent such as dichloromethane. Match each statement to the appropriate category. least polar tert-Butylbenzene elutes first most polar elutes last Answer Bank Acetophenone
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- A gas chromatography column containing a poly(dimethylsiloxane) stationary phase is used to separate the molecules listed. Place the molecules in the order they will elute from the column. Refer to a list of retention indexes for several molecules. Elutes first hexane benzene heptane 2-pentanone 1-nitropropane octane Elutes last Anawer BankHow complexation of diclofenac sodium with hydroxy propyl beta-cyclodextrin by high pressure liquid chromatographic (HPLC) method can enhance the solubility of diclofenac sodium.Which two of compounds below will be difficult to be separated by column chromatography (using ethanol as solvent) and why? -p-anisic acid -methyl-4-aminobenzoate -benzoic acid -ethyl-3-hydroxybenzoate -ethyl-4-nitrobenzoate -trans-cinnamic acid -benzanilide -2-methyl-1-nitronapthalene -9-fluorenone -naphthalene -trans-stilbene -vanillin
- The surface of the silica gel has a structure that looks like this diagram below. Suppose you used a plate coated with silica gel, with propanone, CH3COCH3, as the solvent for thin-layer chromatography. Sno Suppose also that the mixture you were trying to identify contained • benzaldehyde •benzanilide • phenol 1) rank the Rf Value from large to small 2) name the IMF between each compound and the silica gel OH OH main body of silica structureA4 Draw a hypothetical TLC chromatogram of anisole and p-methyl propiophenone in normal phase silica gel taking into consideration the polarity of the two compounds.1) A mixture of benzoic acid, para-xylene and benzaldehyde is spotted on a silica gel TLC plate and developed using Ethyl Acetate with 0.5% acetic acid the solvent. Predict the relative Re values for the three components in the sample (highest to lowest). 2) Arrange the following solvents in order of increasing polarity (least polar to most polar): Diethyl Ether, Propyl Amine, 3-Methyl-Pentane, Acetone
- A thin-layer chromatography (TLC) experiement was run using three compunds: benzophenone, benzhydrol, and biphenyl. The three solvents that were used with the three compounds were hexane, ethyl acetate, and dichloromethane. Which solvent would be the best for separating these compounds and why?The surface of the silica gel has a structure that looks like this diagram below. Suppose you used a plate coated with silica gel, with propanone, CH3COCH3, as the solvent for thin-layer chromatography. Suppose also that the mixture you were trying to identify contained benzaldehyde benzanilide phenol 1) rank the Rf value from large to small 2) name the IMF between each compound and the silica gel OH OH OH -0-Si-O-Si-O-Si-O- O main body of silica structureIdentify these statements with true or false true or false I. Chromatography utilizes partition coefficient theory true or false II. In reverse phase chromatography, the mobile phase is a polar solvent true or false I. Aspirin is an ester prodrug. true or false Aspirin is synthesized by means of an acid catalyzed reaction. true or false I. Grain alcohol boils at 78.3 C true or false Wood alcohol boils at 60 C true or false Diazo coupling involves a sigma and a pi bond in two nitrogen atoms true or false When methyl orange passes through from basic to acidic medium, the change in color is from yellow to intense red true or false Benzoic acid is a white crystalline substance that has a melting point of 122 C true or false Benzoic acid can be prepared by the alkaline reduction of toluene true or false Potassium permanganate is an oxidizing agent true or false Potassium permanganate should never be mixed with glycerin true or false Methyl…
- 2. A Tlc plate was run on the fractions (#1-4) collected from a silica gravity column chromatography that was done. The Tlc plate was developed in 20% ethyl acetate/80% hexanes and is shown below. -------- Fraction # 1234 The compounds that were isolated in each fraction are shown below. Label each compound with the number of the fraction that it was likely found in, based on the TLC results. For example, was compound A found in fraction #2? If so, write A = #2 fraction. Do the same for all 4 compounds. CH₂CH3 .CH3 OH og OH D B CH₂Which is the decolorizing agent used in acid fast staining? Mixture of 5% HCl and 95% NaOH mixture of 5% HCl and 95% ethyl alcohol. mixture of 5% HF and 95% acetone mixture of 5% acetone and 95% alcoholWith the methods and GC columns used in organic labs, the volatility of the nitrotoluene products is the factor that is most responsible for the separation of the products in the GC. Predict the order of the nitrotoluene retention times expected in your chromatogram. Order from lowest (top) to highest (bottom) retention time. Para-Nitrotolulene, Meta-Nitrotolulene, Ortho-Nitrotolulene