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A mutant monkey was identified that showed resistance to a viral disease 'Kansah'. The kan gene, responsible for the disease resistance, was identified and cloned. A molecular technique revealed the presence of kan in human cells. Later these researchers isolated RNA from liver, kidney, gut and skin cells. The researchers prepared CDÑA using reverse transcriptase and then performed real-time PCR to measure the expression of the kan gene. The Ct values for samples from gut, kidney, liver and skin cells were 23, 21, 20 and 40, respectively. Which cells have the highest expression of kan? If a liver cell makes 2000 molecules of Kan protein, assuming equal translation efficiencies in all cell types how many molecules will you expect from a single kidney cell? gut cell?

A mutant monkey was identified that showed resistance to a viral disease 'Kansah'. The kan gene, responsible for the disease resistance, was identified and cloned. A molecular technique revealed the presence of kan in human cells. Later these researchers isolated RNA from liver, kidney, gut and skin cells. The researchers prepared CDÑA using reverse transcriptase and then performed real-time PCR to measure the expression of the kan gene. The Ct values for samples from gut, kidney, liver and skin cells were 23, 21, 20 and 40, respectively. Which cells have the highest expression of kan? If a liver cell makes 2000 molecules of Kan protein, assuming equal translation efficiencies in all cell types how many molecules will you expect from a single kidney cell? gut cell?

Human Anatomy & Physiology (11th Edition)
Human Anatomy & Physiology (11th Edition)
11th Edition
ISBN:9780134580999
Author:Elaine N. Marieb, Katja N. Hoehn
Publisher:Elaine N. Marieb, Katja N. Hoehn
Chapter1: The Human Body: An Orientation
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A mutant monkey was identified that showed resistance to a viral disease 'Kansah'. The kan gene, responsible for the disease resistance, was identified and cloned. A molecular technique revealed the presence of kan in human cells. Later these researchers isolated RNA from liver, kidney, gut and skin cells. The researchers prepared CDNA using reverse transcriptase and then performed real-time PCR to measure the expression of the kan gene. The Ct values for samples from gut, kidney, liver and skin cells were 23, 21, 20 and 40, respectively. Which cells have the highest expression of kan? If a liver cell makes 2000 molecules of Kan protein, assuming equal translation efficiencies in all cell types how many molecules will you expect from a single kidney cell? gut cell?
Transcribed Image Text:A mutant monkey was identified that showed resistance to a viral disease 'Kansah'. The kan gene, responsible for the disease resistance, was identified and cloned. A molecular technique revealed the presence of kan in human cells. Later these researchers isolated RNA from liver, kidney, gut and skin cells. The researchers prepared CDNA using reverse transcriptase and then performed real-time PCR to measure the expression of the kan gene. The Ct values for samples from gut, kidney, liver and skin cells were 23, 21, 20 and 40, respectively. Which cells have the highest expression of kan? If a liver cell makes 2000 molecules of Kan protein, assuming equal translation efficiencies in all cell types how many molecules will you expect from a single kidney cell? gut cell?
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