A total of 5 mL of blood samples were treated with trichloroacetic acid to precipitate proteins. After centrifugation, the resulting solution is adjusted to pH 3 and extracted with 5 ml. of methyl isobutyl ketone containing the ammonium pyrolydine dithiocarbamate (APDC) complexing compound. The extract was then measured its absorbance using an atomic absorption spectrometer (AAS) at 7. 283.3 nm with an absorbance of 0.502. A total of 5 mL of standard Pb solution with concentrations of 0.4 and 0.6 ppm was also given the same treatment and resulted in absorbance of 0.396 and 0.599 ppm. Determine the pb concentration in the sample in ppm

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3. A total of 5 mL of blood samples were treated with trichloroacetic acid to precipitate
proteins. After centrifugation, the resulting solution is adjusted
to pH 3 and extracted with 5 mL of methyl isobutyl ketone containing the
ammonium pyrolydine dithiocarbamate (APDC) complexing compound. The extract was
then measured its absorbance using an atomic absorption spectrometer (AAS) at 7.283.3
nm with an absorbance of 0.502. A total of 5 mL of standard Pb solution with
concentrations of 0.4 and 0.6 ppm was also given the same treatment and resulted in
absorbance of 0.396 and 0.599 ppm. Determine the pb concentration in the sample in ppm
units!
Transcribed Image Text:3. A total of 5 mL of blood samples were treated with trichloroacetic acid to precipitate proteins. After centrifugation, the resulting solution is adjusted to pH 3 and extracted with 5 mL of methyl isobutyl ketone containing the ammonium pyrolydine dithiocarbamate (APDC) complexing compound. The extract was then measured its absorbance using an atomic absorption spectrometer (AAS) at 7.283.3 nm with an absorbance of 0.502. A total of 5 mL of standard Pb solution with concentrations of 0.4 and 0.6 ppm was also given the same treatment and resulted in absorbance of 0.396 and 0.599 ppm. Determine the pb concentration in the sample in ppm units!
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