Please answer parts d and e Acll 2297Xmnl 2294Bcgl 2215Scal 2177Pvul 2066Avall 2059BsTDI 1935Acll 1924Fspl 1919-Avall 1837NmeAIII 1822Bgll 1813-Bpml 1784BsRFI 1779Bsal 1766BciVI 2542Aatll - Zral 2617Sspl 2501BsrDI 1753Bmrl 1744BsmBI 51BsmBI 2683Eco01091 2674Ahdl 1694PUC192,686 bpBceAl 1292Drdl 91oriBstAPI 179Ndel 183Kasl - Narl - Sfol 235Bell 245Fspl 256laczoAlwNI 1217Pvul 276Pvull 306Bmrl 364BseYI 1110BceAl 387MCSBeiVI 1015AflIII - Pcil 806Drdl 908Apol- EcoRI 396Banll - Sacl - Eco53KI 402Acc651- Kpnl 408Aval - BsoBI - Smal-TspMI-Xmal 412Bamil 417Xbal 423Accl - Hincll-Sall 429BspMI-BfuAl 433Sbfl 434Pstl 435Pvull 628Tfil 641BsaXI 659BspQI-Sapl 683Til 781Sphl 441HindIII 447a) The plasmid codes for three elements, lacZ, ApR and ori. What are these threethings?b) Which restriction enzyme cut sites would you use to clone your blue pigment geneinto pUC19 and why?c) You digest the plasmid and your blue pigment gene with the restriction enzymeschosen in step b. Explain how the two fragments will stick together, and explainhow you will stich the phosphate backbone back up.d) Following transformation of the plasmid, how would you ensure that the bacteriayou isolate have successfully taken up the plasmid?e) How would you know which bacteria have a plasmid with your gene inserted?

The insertion of a gene into a foreign gene is known as gene cloning or genetic engineering.…

Acll 2297 Xmal 2294 Bcgl 2215 Scal 2177 Pvul 2066 Avall 2059 BsrDI 1935 Acil 1924 Espl 1919- Avall 1837 NmeAIII 1822 Bell 1813 Bpml 1784- Bsrfl 1779 BcVI 2542 Sspl 2501 Bsal 1766 BsrDI 1753 Bmrl 1744 Aatll-Zral 2617 Ahdl 1694 BsmBI 51 BsmBI 2683 Eco01091 2674 PUC19 2,686 bp BoeAl 1292 ori BstAPI 179 Ndel 183 Kasl - Narl - Sfol 235 Bell 245 Fspl 256 laczo AlwNI 1217 Pvul 276 Pull 306 Bmrl 364 BeYI 1110 Boel 387 MCS BaiVI 1015 AflIIl-Pcil 806 Drdl 908 Apol-EcoRI 396 Banll-Sacl-Eco53KI 402 Acc651-Kpnl 408 Aval - BsoBI-Smal- TspMI-Xmal 412 Bam 417 Xbal 423 Accl - Hincll-Sall 429 Pull 628 Til 641 BsaXI 659 BspQl-Sapl 683 Til 781 BspMI-BfuM 433 Sbfl 434 Pstl 435 Sphl 441 HindIII 447 a) The plasmid codes for three elements, lacZ, ApR and ori. What are these three things? b) Which restriction enzyme cut sites would you use to clone your blue pigment gene into pUC19 and why? c) You digest the plasmid and your blue pigment gene with the restriction enzymes chosen in step b. Explain how the two fragments will stick together, and explain how you will stich the phosphate backbone back up. d) Following transformation of the plasmid, how would you ensure that the bacteria you isolate have successfully taken up the plasmid? e) How would you know which bacteria have a plasmid with your gene inserted?

11th Edition

ISBN:9781305143531

Author:CURREN

Publisher:CURREN

Chapter5: Unit, Percentage, Milliequivalent, Ratio, And Household Measures

Section: Chapter Questions

Problem 1.1P