An appropriate biochemical buffer should have charges in both conjugate acid and conjugate base form. This avoids the presenc of a neutral form of the buffer that might be able slowly leak into cells. Does unmodified dimethylpiperazine meet this criterion when buffering at pH 8? o Yes No What about buffering at pH 4? O Yes No
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- Your running buffer in SDS-PAGE consists of a Tris, SDS and glycine. Why can you not just use distilled water?At what pH does a lysine solution exhibit the highest buffering capacity? (Lysine pKas: pk1 = 2.2, pK2 = 8.95, pK3 = 10.5) %3D O рH 12.1 О рH 9.72 O pH 5.67 pH 8.95Calculate the volume of TAE buffer that you will need to prepare a 100ml solution of 1X strength if you only have a 50X concentrated solution available.
- How to create a 750 uL of XS buffer (with compositions 1% potassium ethyl xan- thogenate; 100 mM Tris-HCl, pH 7.4; 1% sodium dodecylsulfate; 800 mM ammonium acetate)? Show calculationsTris NaCl buffer is known as?N-(2-hydroxyethyl)piperazine-N'-(2-ethanesulfonic acid) buffer at pH 7 with 400 mM NaCl. A A purified protein is in a Hepes dialysis membrane tube holds a 5.0 mL sample of the protein solution. The sample tube floats in a beaker containing 0.50 L of the same Hepes buffer, but with 0 mM NaCl, for dialysis. Small molecules and ions (such as Na*, CI", and Hepes) can to diffuse across the dialysis membrane, but the protein cannot. Assume there are no sample volume changes during the dialysis. Calculate the final concentration of NaCl in the protein sample once the dialysis has come to equilibrium. Calculate the final NaCl concentration in the 5.0 mL protein sample after dialysis in 300 mL of the same Hepes buffer, with 0 mM NaCl, twice in succession. [NaCl] after a single dialysis: [NaCl] after a double dialysis: mM mM
- You have been provided with stock solutions of: stock A: 0.06 M sodium pyrophosphate buffer pH 8.5 stock B: 3 M ethanol stock C: 0.015 M NAD+ stock D: milli Q water Determine the volume you will need of each solution to prepare a buffer of with a final volume of 60 mL containing 10 mM sodium pyrophosphate pH 8.5, 100 mM ethanol, 1 mM NAD+. i.e. volume of stock A = _________mL volume of stock B = _________mL volume of stock C = _________mL volume of stock D = _________mL Show your calculations to arrive at your answers.It is possible to make a buffer that functions well near pH7 using citric acid, which contains only carboxylate groups. Explain.10 tab of acetaminophen 500 mg molar absorptivity= 14520 @ 243nm. The total weight of the 10tab is 5278 mg. I take a sample equivalent to 100mg of tablet and I diluted it in 100mL, I took an aliquot of 10mL and I diluted it in 250mL, of these I took 25mL and I diluted them in 250mL. Calculate the weight per tablet. Absorbance = 0.944
- The pl of alkaline phosphatase is 4.5; the pl of the DEAE cellulose is 10.5. We used a buffer of pH 7.4 to run a DEAE-cellulose as anion exchange column? Explain how did this work?Based on our pka's table, pKa1 of -COOH group of Cys = 1.71 pKa2 of -NH2 group of Cys = 10.78 pKa3 of side chain group of Cys = 8.33 If pKa is different, will the buffer change??A base is added to a 0.050 M solution of MnCl2, raising the pH gradually. Calculate the pH of the solution when 88 of the manganese has precipitated? Table 5-7 Solubility-Product Constants, K, at 25°C Fluorides Chromates (cont.) Ag, Cro, PbCro, Hydroxides (cont) Ni(OH), Zn(OH), Cu(OH), Hg(OH), Sn(OH), Cr(OH), A(OH), BaF, MgF, PbF, SrF, CaF, 2.4 x 10-5 X 10-8 x 10-8 1.9 x 10-12 1.6 x 10-16 4.5 X 10-17 1.6 x 10-19 3 2 x 10-16 4 Carbonates 7.9 x 10-10 3.9 x 10-11 X 10-26 NiCO, CaCO, BaCo, SrCO, CuCO, ZnCO, MnCO, 1.4 x 10-7 3 10-27 4.7 x 10- 6.7 x 5 x 6 x 10-38 2.5 x 10-43 Chlorides 10-31 1.6 x 10- 1.7 x 10-10 1.1 x 10-18 1.6 x 10-9 7 x 10-10 2.5 x 10-10 10-33 PBCI, AgCi Hg,Cl, Fe(OH), Co(OH), Sulfides 2 x 10-10 8.8 x 10-11 2.1 x 10-11 Bromides X 10-16 X 10-19 3 x 10-21 X 10-22 2.5 x 10-22 X 10-26 1.0 x 10-28 MnS 7. PbBr, AgBr Hg, Br, 4.6 x 10-6 5.0 x 10-13 Feco Ag,CO, CdCo, PBCO, MgCO, Hg,CO, Fes 4 8.2 x 10-12 5.2 x 10-12 1.5 x 10-15 1 x 10-15 NiS 1.3 x 10-22 Cos ZnS lodides Pbl, Agl…