Answer the following questions: Why are most cells so small? Why cell size is limited? List two instances when the coarse adjustment knob is never used Why is immersion oil used with the 100X objective? List two common problems associated with using the microscope and how you would go about solving it. When should the lenses be cleaned? What is the correct way to clean them?

Answer the following questions: Why are most cells so small? Why cell size is limited? List two instances when the coarse adjustment knob is never used Why is immersion oil used with the 100X objective? List two common problems associated with using the microscope and how you would go about solving it. When should the lenses be cleaned? What is the correct way to clean them?

Basic Clinical Laboratory Techniques 6E

6th Edition

ISBN:9781133893943

Author:ESTRIDGE

Publisher:ESTRIDGE

Chapter1: The Clinical Laboratory

Section1.10: The Microscope

Problem 18RQ

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Similar questions

Shown below are several micrographs (images from microscopes) that were taken using different microscopy techniques. Write down what method of microscopy was used and why you thought so. Choices for microscopy techniques are: Transmitted Light Microscopy (techniques of brightfield, phase-contrast, darkfield, DIC all fall under this), Fluorescence Microscopy, Scanning Electron Microscopy, and Transmission Electron Microscopy.
Which of the following statements are true in the context of the microscope's field of view? I. It is the observable area within a circular space as defined by the lenses II. FOV is the smallest on the lowest power objective III. FOV is the maximum area visible through the microscope eyepiece IV. When you switch to a higher power, the field of zooms out towards the center
Complete Table 1 below by supplying the characteristics of each objective. Table 1. Numerical characteristics of microscope objectives. LPO НРО OIO Focal length (mm) Working distance (mm) Linear magnification (X) Numerical aperture (N.A..) Features (color of the band, diameter of the opening of the lens, etc.)
Assume you are observing the diatom pictured in Figure 1 using the 10X lens in a compound light microscope. You move to the 40X lens and then again to the 100X lens by only rotating the turret (remember that the lenses are parfocal), without making any other adjustments to the microscope. c) After making your adjustments, you notice that the midline of the diatom is in focus while the remainder is blurry. Explain, based on microscopy principles, why this has occurred Provide a description of the procedure to prepare an acidic stain of bacteria using Nigrosin as it would appear in the Methods section.
Please answer the following questions (Question 1 AND 2) about compound microscopes. After clicking the objective into position, which adjustment knob(s) can you use to safely refocus the image? You may need to adjust the light intensity to better see the specimen. Indicate two ways in which you can change the light intensity?
What information can be obtained from the slides prepared by wet mount and hanging drop technique for microscopic observation? Give at least three (3) information. What are the limitations of each method. (Provide 2 limitations)
How is xylol used to clean the lens of a microscope? What may happen if xylol is constantly used for this purpose? For what purpose would you adjust each of the following microscope components during a microscopy exercise? iris diaphragm coarse adjustment knob fine adjustment knob condenser mechanical stage control 3. Upon observation of the nutrient agar slant culture, you strongly suspect that the culture is contaminated. Outline the method you would follow to ascertain whether your suspicion is justified.
Using the microscope Answer the following questions as you work through this exercise: 1. How is the letter “e” on the slide oriented when you see it with the naked eye as you mount it on the stage (i.e., is it right side up or upside down)? 2. How is the letter “e” on the slide oriented when you see it under low or high power magnification? 3. What effect, if any, does the compound light microscope have on the orientation of the image?
How will the following affect resolution during microscopy? I) Closing or opening the diaphragm II) Raising or lowering the condenser III) Increasing or reducing the light intensity
3D dimensionality is a limitation of the compound microscope. Depth of field, DOF, describes dimensionality form top to bottom and can be observed with colored cross threads. Observe the crossed thread slide on low power (4x), then on medium power (10x), then on high power (40x objective magnification). Which crossed fiber is on top? How do you know?
Discussion: 1) What does calibrating the microscope mean? Why do we calibrate the microscope? 2) What is the ocular calibration scale for each objective? Why is important to calibrate each objective?
Can someone answer and explain the following? What are the basic components of a Fluorescence Microscope and what are the functions of each? Are there any parts that you can remove without compromising accuracy and utility of the equipment? Can you suggest additional components to improve the equipment?