As in the previous question: Using BSA concentrations of 0 mcg/mL, 1 mcg/mL, 3 mcg/mL, 4 mcg/mL, and 5 mcg/mL you get the following absorptions: 00.2, 0.12, 0.32, 0.42, 0.52. You dilute your sample 1:5 and get an absorbance of 0.15. What is the concentration of your sample? O a. 1.3 mog/ml b. 6.5 mcg/ml c. 0.035 mcg/ml d. 0.175 mcg/ml
Q: Weight of egg yolks: 17.8 g Crude cholesterol weight: 232 mg Recrystallized cholesterol weight: 168…
A: Amount of egg yolks = 17.8 gm = 17800 mg Amount of crude cholesterol = 232 mg Amount of…
Q: Below is the procedure described for determination of paracetamol according to BP: "Weigh and powder…
A: Interpretation: To identify the correct statement about assay of paracetamol.
Q: What is the absorbance given that the absorptivity is 2.30 x104L mol-1cm-1, the pathlength is 1.00…
A: Given data ; 1) absorpitivity or molar absortion coffecient = 2.30 * 104 L.mol-.cm- pathlength =…
Q: Your lab partner gets a little confused and prepares a dilution of your protein sample by combining…
A: In case of dilution of solutions, we may use the expression: c1V1=c2V2where,c refers to the…
Q: A solution of FESCN2+ has a concentration of 1.00 × 10-2M and an absorbance of 0.755. What is the…
A: Introduction: Beer's Lambert law shows the linear relationship between the concentration of the…
Q: If you have a sample which has an absorbance of 1.17, a molar absorptivity of 1 Mcm1, and a cuvette…
A: The given data contains, Absorbance=1.17path length=1.15 cmMolar absorptivity=1 M-1.cm-1
Q: In what order would the following compounds be eluted on a silica gel TLC plate if they are spotted…
A: Rf value is the ratio of the distance travelled by the analyte and the distance travelled by the…
Q: ) Identify the calibration method used. b) Calculate the molar concentration of morphine in the…
A: Given: concentration and absorbance when measuring morphine at 285 nm using a 1.00 cm cuvette. To…
Q: 1. How do the amount of peaks differ? 2. Compare the range of PPM 3. Are there any other differences…
A: Since you have posted a question with multiple sub-parts, we will solve first three subparts for…
Q: A TLC is developed using a mixture of acetone/methanol (both polar solvents) as mobile phase. The…
A:
Q: A student tested water with 2% NaCl as a possible solvent for the separation of a mixture that could…
A: 2% of NaCl is the possible solvent for the separation of a mixtures G3 Y5 R3 B1. a) The distance…
Q: 1) Which technique would be best to separate and quantitate two organic compounds with differing…
A: “Since you have asked multiple questions, we will solve the first question for you. If you want any…
Q: Return 2pnts Below is a developed TLC plate (1:1 hexanes ethyl acetatel. The four standards in the…
A:
Q: Consider the colors found in the orange pen. Choose the Rf value closest to that of the spot that…
A: A question based on analytical separations that is to be accomplished.
Q: A sterile ophthalmic suspension contains 0.5% timolol maleate (m.w. 433). How many milligrams of the…
A: We have 0.5% w/v opthalmic solution. Solute here is timolol maleate. Mol mass of timolol maleate =…
Q: This is an output from an HPLC experiment. The column was packed with extremely hydrophobic grains.…
A: HPLC High performance liquid chromatography . This technique is based on the adsorption by which the…
Q: You performed a Bradford Assay and the standard curve is shown below. 1. The absorbance of your…
A: Here according to the given curve : Relationship of straight line is y = 1.5369x And range of…
Q: Question 1 In an experiment to determine the riboflavin by fluorescence spectrometry, a series of…
A: Hence option c is correct that is 0.725
Q: Match column A with Column B. Given: Samples were spotted on the TLC plate and after performing the…
A: This question is related to Thin Layer Chromatography. Thin-layer chromatography (TLC) is a…
Q: order to help identify the things in a chromatogram, you can measure the Rf value for each spot. How…
A: Various techniques are used for the analysis of compounds in chemistry. Chromatography is one of…
Q: A student
A: she gets a R of 0.69 and for the red spot, she gets a R of 0.45from the given data she analyses that…
Q: what is the CFU per
A: Y=mx+c where Y is absorbance and X is the concentration and C is intercept made by line on the…
Q: What would be the concentration of a solution made by diluting 20,0 mL of 5,5 NaOH to 100 mL?…
A: The required reaction is given by, C1V1=C2V2 where C is the concentration and V is the volume.
Q: 3) 3-Methylbutyramide 100 CHINO 101 R28888 CH₂N relative intensity relative abundance 4)…
A:
Q: Help me solve this, please? With the following data, and given the absorbance of the unknown…
A: The concentration (M) of the unknown solution is to be calculated
Q: Whagt is the purpose of the following? Separate the active ingredients in an unknown over the…
A: Thin-layer chromatography is a technique for separation of compounds based upon the difference in…
Q: A health care provider ordered sulfasalazine (Azulfidine) Oral Susp 500 mg q 6 hr; Directions for…
A: Drug formulation is the mixture containing active component along with inert components. The dosage…
Q: What is the absorbance of a theoretical compound in a 5.26 x 10-5 M solution, a path length of 1.5…
A: The relationship between the absorbance and concentration is given by A = ECl where A = absorbance…
Q: Caffeine (C8H10O2N4•H2O) had an absorbance of 0.510 to 272 nm in 1.0 cm optical path in…
A: Given: Concentration of caffeine, (C) = 1.0 mg/100 ml. The molar mass of caffeine, (M)=212.09 g/mol.…
Q: student runs a TLC of a sample using a nonpolar development solvent. The TLC shows one spot. The MP…
A: Given is, A student runs a TLC of a sample using a nonpolar development solvent. The TLC shows one…
Q: Given the following concentrations of aqueous ions as well as their atomic weight or molecular…
A:
Q: A solution containing Cu2+ is analyzed by atomic absorption and gives an absorbance of 0.01000…
A:
Q: In 250 ml ethanol solution, a tablet of saccharin was dissolved, and it was measured by UV…
A: Beer's law states that the absorbance is directly proportional to the concentration of the absorbing…
Q: How many milligrams of piroxicam is required to make 5 solid ounces of 1:270 (w/w) piroxicam gel?
A:
Q: Identify three things that are incorrect about the following graph. Absorbance as a Function of…
A: The given graph is of absorbance versus the concentration of ion. Absorption and the concentration…
Q: 5 3 2 |------------ 1 2 3 4 5 4, 1. distance in cm
A: Rf = 0.40
Q: A student ran a TLC plate using a 90:10 water:isopropyl alcohol developer and this was the outcome.…
A:
Q: What is the correlation between the level of the spectrophotometer's absor- bance readings and the…
A: Given table: pH of buffer solution Absorbance 3 1.270 5 0.410 7 0.275 9 0.240 11 1.170
Q: ml Diluted Undiluted Diluted to mL Water Absorbance Unknown Conc. (M) Conc. (M) 0.5 4.5 0.4IS 1.25…
A: Given are the volumes of unknown sample which are diluted with given varying volume of water. The…
Q: Riboflavin, also known as vitamin B2, has a molar absorptivity coefficient of 3 x 10 ^3 . If the…
A: given; Absorbance A = 0.90 molar absorptivity coefficient = 3 x103 Concentration of riboflavin = ?
Q: QUESTION 1 You obtained the following raw data when setting up a Biuret standard curve: BSA (mg/ml)…
A: Given: absorbance of unknown sample = 0.284 To find: Set up a standard curve, determine the protein…
Q: 8) Rank the following compounds in order from lowest to highest retention time in GC (using a OV-101…
A: Gas chromatography (GC) is an analytical instrumental method that is used for the…
Q: How many milliliters of sulfuric acid labeled 96% w/w, (sp.gr. 1.80) will be needed to make 12 fl oz…
A: Given: Concentration in %(w/w) of stock solution = 96 % Specific gravity of stock solution = 1.80…
Q: Can you help me on questions 2 AND 3
A: To solve this problem we can use beer’s law equation:
Q: What type of compound corresponds to the IR spectrum shown here? MICRONS 25 2.62.72.82.9 3 100 3.5…
A: IR spectrum is used to study and identify chemical substances or functional groups present in them…
Q: A student tested water with 2% NaCl as a possible solvent for the separation of a mixture that could…
A: 4 a) The distance of the solvent front can be measured by a ruler. For this, match zero on your…
Q: You were assigned to determine the concentration of an unknown starch solution. [This unknown is…
A: Calculation of concentration (C = A/(L x Ɛ))The Lambert-Beer law, which forms the physical basis…
Q: To 4-5 drops of an unknown alcohol, 1 mL of Lucas reagent (ZnCl2 + HCl) is mixed. No cloudiness or…
A: Lucas reagent is made up of ZnCl2 mixed with HCl. When Lucas reagent is mixed with an primary…
Q: A student dissolved 16.7 mg of sunscreen in 25 mL of 2-propanol. They then diluted this stock…
A: The answer is attached below-
Trending now
This is a popular solution!
Step by step
Solved in 2 steps with 1 images
- Solution Concentration of FeSCN2+ (M) Absorbance1 0.0000491 0.3112 0.0000818 0.5763 0.000115 0.7554 0.000152 0.9075 0.000196 1.185 Solution Concentration of FeSCN2+ (M) Absorbance 1 0.0000491 0.311 2 0.0000818 0.576 3 0.000115 0.755 4 0.000152 0.907 5 0.000196 1.185 A series of aqueous solutions of FeSCN2+ of different concentrations were prepared, and the absorbance of each was measured at a wavelength of 455 nm. Use Excel or Google Sheets to prepare a useful graph of the data (given below) that could be used to determine the concentration of an unknown solution from its absorbance. Ensure your graph is properly formatted and labeled. Click the…q41 IF YOU DO NOT KNOW THE ANSWER PLEASE DO NOT REJECT IT please calculate the unknown concentration of the protein D wih an absorbance value of A412 given the standard curve indicated in the table. write your answers in numbers only with 2 decimals. protein concentration (ug/ml) absorbance 0 0.000 0.02 0.161 0.04 0.284 0.06 0.438 0.08 0.572 0.10 0.762Consider the following data that was collected for a protein that contain 0.76 % tryptophan amino acids by weight (Molecular weight of tryptophan amino acids is 204 g/mol). Absorbance Concentration (mg/L) 0.0250 0.100 0.150 0.226 0.259 0.400 0.600 0.964 1.14 1.78 1.45 2.35 1.78 2.87 How many mg of the protein present in 1.00 L solution that shows an absorbance of 1.20?
- If the lime recommendation for 1-hectare of land is 6 tons of pure CaCO3, how much of Ca(OH)2 will you need if you use it instead, but is only 70% pure? (RNV of CaCO3 = 100; RNV of Ca(OH)2 = 136) %3D Select one: a. 10.5 tons b. 5.7 tons C. 7.45 tons d. 6.3 tonsq42 please calculate the unknown concentration of the protein D wih an absorbance value of A554 given the standard curve indicated in the table. write your answers in numbers only with 2 decimals. protein concentration (ug/ml) absorbance 0 0.000 0.02 0.161 0.04 0.284 0.06 0.438 0.08 0.572 0.10 0.762No. 1 2 Predict the structural formula for each set of data (cl 3 4 Molecular formula C6H₁1O₂Cl C7H1402 CH₂ C9H12 C7H140 V (cm³¹) 1734 strong 3300 broad 1730 PH 1710 "5" 8 (ppm) CH₂ CH H Mark) 1.32(d, 6H), 2.53(t,2H), 3.9(t,2H), 4.93 (septet, 1H) 1.18(d,3H), 1.44(quartet,2H), 1.57(quintet,2H), 2.13 (s ,3H), 2.45(t,2H), 4.03( sextet,1H), 3.58(s, 1H) 1.2(d,6H), 2.87(septet,1H), 7.23(s,5H) 1.01(d,12H), 2.45(septet, 2H)
- explain the results of this experiment table 2 Test Tube Number Cobalt Chloride Concentration (mol/mL) Absorbance at 510 nm 1 0.000 0.000 2 0.009 0.042 3 0.018 0.92 4 0.027 0.139 5 6 0.036 0.045 0.190 0.236 7 0.029 mol/mL 0.151QUESTION 4 You obtained the following raw data when setting up a Biuret standard curve: BSA (mg/ml) Absorbancy 540nm 0.158 1 0.210 0.260 3 0.305 4 0.360 0.410 0.455 0.510 8 0.530 0.550 10 0.554 What would the quality of the line-fit (R2 value) be if you do not exclude experimental outliers? (Give you answer to 4 decimal places)Sultan Qaboos E ES : Standard Solution 3 Conc. Of PO, 3- (ppm) 0.00 0.2 0.4 0.6 0.8 1.0 Absorbance 0.00 0.033 0.069 0.107 0.134 0.155
- Three alcohol bottles were mislabeled in the stockroom: 1-pentanol, 2-pentanol, and 3-pentanol. A clever student decided to using MS to determine the identity of the structures. Using the MS data, draw the structure of the compound found in each bottle. Bottle #1: Base peak with a m/z of 45 Bottle #2: Base peak with a m/z of 70 and two significant peaks with m/z of 42 and 31 Bottle #3: Base peak with a m/z of 59Sample (μg) Absorbances (520 nm) Concentration 0.0 0.621 0 2.5 0.599 2.5 5.0 0.556 5 10.0 0.469 10 15.0 0.418 15 20.0 0.291 20 Plot a graph in which y axis represents for absorbance and x-axis represents for concentration From graph, y=mx + c Calculate the vitamin C concentrations of samples by the equation with consideration of dilution factors.8. Chemical Formula: C9H17NO5 8 1H, broad 7 6 Final Answer 1H, broad -5 elle 4 PPM 3H s 3 2 9H s 1 there are 3 peaks between 4.00 and 4.30 ppm: a 1H t at 4.3 ppm two 1H t that both happen to be at 4.0 ppm, but are not the same 0