At pH 10, [OH-] is— 10 M 5 M 10-10 M 10-4 M 0.0000001 M
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At pH 10, [OH-] is—
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10 M |
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5 M |
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10-10 M |
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10-4 M |
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0.0000001 M |
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- Calculate the concentration of Y4-ion in 0.0100 M EDTA solution at pH 6. α4 = 2.2 x 10-5 for pH 6.80-000 A7000 MN KANAMYCIN INJECTION, USP *1 g/3 mL (333 mg/mL) For IM or IV Use 3 mL Vial Rx only 10 Vials Using the label above, how many mL of Kanamycin must be administered to a patient in which the orders read 300 mg IM stat. Give your answer to 2 decimal places. Type mL in the units box. Your Answer: Sterie "Each mL contains lenamycin sulate equiralent to 333 mg kanamycia. 0.45% sodium bisulfite added as an antioxidant, buffered with sodium citrate (2.2 %). Adjusted to pH 4.5 with H,S0, Kanamycin Injection should not be phys ically mixed with other antibaderial agents Usual Dosage: Intramuscubr 7.5 ng/ig of bady weight at 12-hour intervals. Do not axceed 1.5 grams daily. See insert for IV and other uses Vial stoppers do not contain natural nubber latex. Store at 20 to 25 C (68 to 77 F) [see USP Contrdled Room Temperature]. Avoid freezing. APP APP Pharmaceuticals, LLC Schaumburg, IL G0173 63323-359-03 42650D LOT/EXP8 7- 6- 2- 1 405 180 225 270 315 [Substrate] (nM) 45 90 135 360 450 495 540 58! What are the units of Km from the plot shown above? nanometers O nM sec/nM O not enough information to tell O nM/sec rate (nM/sec) 3.
- 9. 3 μL of a 45 mM stock solution of a substrate is added to 8 mL of water. Calculate the following values. The substrate molecular weight is 125 g/mol. Substrate volume in mL Dilution factor Substrate number of moles Substrate concentration for the diluted solution in mM Substrate concentration for the diluted solution in mole/L Substrate concentration for the diluted solution in mg/mLpH of solution 14.00 12.00 10.00 8.00 6.00 First 4.00 equivalence point 2.00 0 First midpoint Second equivalence point Third midpoint pH = pKa=12.32 Third equivalence point HPO4(aq) + OH(aq) PO4(aq) + H2O(1) Second midpoint pH = pKa = 7.21 H2PO4 (aq) + OH(aq) HPO42 (aq) + H2O(1) Using the Henderson- Hasselback equation, show how to create 2L of a 0.1 M KPhos pH 7.5 buffer using K2HPO4 and KH2PO4. The chart to the left should help you understand what pKa to start with. Show your work. pH = pKa = 2.16 H3PO4(aq) + OH(aq) H2PO4(aq) + H2O(l) 25.0 50.0 75.0 100.0 Volume of NaOH added (mL)What mass of sodium glycolate (NaC2H3O3) should be added to 400.0 mL of 1.00 M glycolic acid to produce a buffer solution with a pH of 4.00? Ka = 1.47 x 10-4. Please indicate the full solutions.
- What mass of sodium glycolate (NaC2H3O3) should be added to 400.0 mL of 1.00 M glycolic acid to produce a buffer solution with a pH of 4.00? Ka = 1.47 x 10-4The pH of a 0.0082 M solution of HNO₃ isSize Size Crude Anion Cation 2) Exclusion Exclusion Lysate Exchange Exchange 1 2 Total Protein Concentration 15.2 6.6 2 3.75 4.7 (mg/mL) Final Sample Volume 60 30 20 4 3 (mL) Enzyme Specific Activity 0.43 1.7 7.25 18.3 12.5 (units/mgprotein) Based on your protein purification sample data, in which of the purification steps in your protocol did you effectively purify your enzyme? Select all that apply. a) anion exchange correct b) cation exchange correct c) size exclusion 1 correct d) size exclusion 2