I need help how to find value for starting sucrose concentration column Jthe reducing sugar I will find by comparing Absorbance to standard curve from previous experiment no need to worry bout G and H columnThank you 123456789101117ATube1234567сBpH 4.5 Buffer (μl) water ul250250250250250250250500450400350300250225DE150m M Sucrose (extract (μl)250250250050100150200250275250250250250FGDNSA μl Absorbance800800800800800800800HReducing sugar μmoles Sucrose added (µmoles) Starting sucrose concentration [S] (mm)07.51522.53037.541.25

Answered: Image /qna-images/answer/9a1bfd9d-44fa-4b50-a6b1-ef8e4283e635.jpg

B с A Tube pH 4.5 Buffer (μl) water ul 250 250 250 250 250 1 2 3 4 5 6 7 250 250 500 450 400 350 300 250 225 D E G 150mM Sucrose (extract (μl) DNSA μl Absorbance 250 800 250 250 250 250 250 250 0 50 100 150 200 250 275 800 800 800 800 800 800 H Reducing sugar μmoles Sucrose added (umoles) Starting sucrose concentration [S] (mm) 0 7.5 15 22.5 30 37.5 41.25

B с A Tube pH 4.5 Buffer (μl) water ul 250 250 250 250 250 1 2 3 4 5 6 7 250 250 500 450 400 350 300 250 225 D E G 150mM Sucrose (extract (μl) DNSA μl Absorbance 250 800 250 250 250 250 250 250 0 50 100 150 200 250 275 800 800 800 800 800 800 H Reducing sugar μmoles Sucrose added (umoles) Starting sucrose concentration [S] (mm) 0 7.5 15 22.5 30 37.5 41.25

EBK A SMALL SCALE APPROACH TO ORGANIC L

4th Edition

ISBN:9781305446021

Author:Lampman

Publisher:Lampman

Chapter47: Benzocaine

Section: Chapter Questions

Problem 4Q

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