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- Suppose you spilled two cultures of Salmonella typhimur-ium (each containing 100,000 cells) on your lab bench. You im-mediately applied the same disinfectant to both cultures at thesame time. One culture had been freshly grown for 36 hours andthe other culture is 2 weeks old. If the kill ing rate of the disin-fectant is 90% per minute, do you think that microbes in bothcultures will be completely killed after 6 minutes? Why or whynot?ITEM MSM MICROBIAL PROFILE I MICROORGANISM/CAUS ATIVE AGENT A GRAM REACTION B OXYGEN REQUIREMENT с SIZE SHAPE HABITAT DISCOVERY G MICROSCOPIC IMAGE || | DISEASE PROFILE DISEASE/S A B SYMPTOMS OF THE DISEASE C INCUBATION PERIOD D MODE OF TRANSMISSION DIAGNOSIS TREATMENT PREVENTION NO OF DAYS BEING SYMPTOMATIC I IMAGE OF INFECTED PATIENT DEFG EFGH G PROFILE Corynebacterium diptheriae1 H 2 176 old culture Smear too BOTERC Losk like? + b. Failure to apply the decolorizer. + ne c. Failure to apply the safranin. Determine whether bacteria are motle or non-motle by looking at the growth in semi-solid medium What is the concentration of agar in (a) typical solid medium (b) semi sold motility medium? Arawer Q1 on Page 357. 7. Orygen Requirement: + d. Reversal of crystal violet and safranin stains. A questions 1.2 and 3 o Pg 83-84 Refer to the power point Questions 1 Predict the effect of the following "mistakes" made when performing a Gram stain. Your answer needs to address the predicted effect on both Gram-positive and Gram-negative cells. Consider each mistake independently. a. Failure to add the iodine. 414 gait to fer. 2 Both crystal violet and safranin are basic stains and may be used to do simple stains on Gram-positive and Gram-negative cells. This being the case, explain how they stain different cell types in the Gram stain. why? a cell wall. patem in…
- “Salt agar” is a type of ______ medium for culturing some microbes and not othersITEM MSM MICROBIAL PROFILE I MICROORGANISM/CAUS ATIVE AGENT A GRAM REACTION B OXYGEN REQUIREMENT с SIZE SHAPE HABITAT DISCOVERY G MICROSCOPIC IMAGE || | DISEASE PROFILE DISEASE/S A B SYMPTOMS OF THE DISEASE C INCUBATION PERIOD D MODE OF TRANSMISSION DIAGNOSIS TREATMENT PREVENTION NO OF DAYS BEING SYMPTOMATIC I IMAGE OF INFECTED PATIENT DEFG EFGH G PROFILE Haemophilus ducreyiWhy do organisms like Lactococcus grow at the bottom of the broth tube and not spread throughout the broth tubes like the other organisms? Check all that apply. they are extremely heavy organisms O they stick together and make this large heavy mass of bacteria O they are dead are cocci and do not possess flagella y are non-motile and gravity pulls them to the bottom O there are more nutrients at the bottom of the tube O O O O
- Day Care Center outbreak: Salmonellosis You just finished identifying the fecal contaminated food at the first restaurant when you learn there is an outbreak of Salmonella-food poisoning at a day care center, You sample food from the Center's refrigerator and are worried that the egg salad or chicken salad or peanut butter used to make sandwiches may be contaminated with Salmonella. You know Salmonella cannot ferment lactose and it can make hydrgoen sulfide. The strain associated with the outbreak cannot ferment sucrose but can ferment glucose. You isolate in pure culture bacteria from each of the suspect foods and inoculate each into TSI slants. (see results below) egg salad microbe chicken salad microbe peanut butter microbe TSI TSI TSI W FWSSpores and toxin-producing microorganism produce toxins need higher Aw than minimum Aw for microbial growth. How could food industry benefit from this fact?Which of the following is true about cell wall of gram-positive bacteria? O Not loss primary stain after alcohol O It consists of multiple peptidoglycan layer O Option 5 O It is thicker than that associated with gram-negative bacteria. All of these
- Taking microbial cells out of a stationary phase culture and putting them into a fresh, sterile medium (in which they can grow) will result in the cells entering which stage of the growth cycle? Death phase O Log phase Lag phase Stationary phaseAgar is added to media. a. many kinds of bacterial O b. All kinds.of viral no kinds of microbial O d. All kinds of microbialA chemical, produced by a microbe to inhibit the growth of other microbes, is isolated and then modified in a chemical reaction in the laboratory. Which of the following terms would most accurately apply? O Synthetic O Antibiotic O Phenolic O Microbistatic O Opportunistic