Bacterial Genetic Transformation: This experiment is attempting to encode for the GFP gene and ampicillin-resistance in bacteria through a genetic transformation. This is done using pGLO. The question is: Frequently, experiments do not turn out as expected and good scientists learn how to "troubleshoot" and test possible reasons for unexpected results. Sally's team ran this experiment but observed no colonies on the +pGLO + ampicillin + arabinose plate. List 3 possible reasons for this - and ways that Sally can test whether these are the cause of these experimental results.
Genetic Recombination
Recombination is crucial to this process because it allows genes to be reassorted into diverse combinations. Genetic recombination is the process of combining genetic components from two different origins into a single unit. In prokaryotes, genetic recombination takes place by the unilateral transfer of deoxyribonucleic acid. It includes transduction, transformation, and conjugation. The genetic exchange occurring between homologous deoxyribonucleic acid sequences (DNA) from two different sources is termed general recombination. For this to happen, an identical sequence of the two recombining molecules is required. The process of genetic exchange which occurs in eukaryotes during sexual reproduction such as meiosis is an example of this type of genetic recombination.
Microbial Genetics
Genes are the functional units of heredity. They transfer characteristic information from parents to the offspring.
Bacterial Genetic Transformation:
This experiment is attempting to encode for the GFP gene and ampicillin-resistance in bacteria through a genetic transformation. This is done using pGLO.
The question is:
Frequently, experiments do not turn out as expected and good scientists learn how to "troubleshoot" and test possible reasons for unexpected results. Sally's team ran this experiment but observed no colonies on the +pGLO + ampicillin + arabinose plate. List 3 possible reasons for this - and ways that Sally can test whether these are the cause of these experimental results.
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