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Base on the result of the biochemical how can you know this is Legionella Pneumoophila vs OTHER bacteria ??
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- Make the Dichotomous key for M. luteus, Bacillus subtilis, bacillus, Megaterium, Bacillus cereus, E.coli, S. Marcescens, and Enterobacter aerogenes based on catalase test, (lactose, sucrose and glucose fermentation tests) and other biochemical test for accurate identification and colony morphology.Cells were incubated with chloroquine for 2 hours before stimulation with lipopolysaccharide (LPS, lg/ml). Cell viability was measured by staining cells with 3-(4,5-dimethylthiazol-2- yl)-2,5- diphenyltetrazolium bromide. TNF in the culture supernatant and the cell lysate were measured by sandwich ELISA, using specific monoclonal antibodies according to the manufacturer's instructions. To determine the level of cell-associated cytokines, cells were washed with phosphate-buffered saline (PBS) and lysed in a buffer containing 0.5% NP-40. Standard recombinant proteins were also diluted in PBS containing the same concentrations of NP-40 as the cell lysate. Cytokine synthesized were proteolytically cleaved and released into medium. The amount of cytokines in the culture supernatant and the cell lysate were presented. Results for TNF production are shown in Figure 1 (Jang et al, 2006). (Cells : PBMC: Human peripheral blood mononuclear cells, monocytes, monocytic lymphoma U-937 and leukemia…Cells were incubated with chloroquine for 2 hours before stimulation with lipopolysaccharide (LPS, 1g/ml). Cell viability was measured by staining cells with 3-(4,5-dimethylthiazol-2- yl)-2,5- diphenyltetrazolium bromide. TNF in the culture supernatant and the cell lysate were measured by sandwich ELISA, using specific monoclonal antibodies according to the manufacturer's instructions. To determine the level of cell-associated cytokines, cells were washed with phosphate-buffered saline (PBS) and lysed in a buffer containing 0.5% NP-40. Standard recombinant proteins were also diluted in PBS containing the same concentrations of NP-40 as the cell lysate. Cytokine synthesized were proteolytically cleaved and released into medium. The amount of cytokines in the culture supernatant and the cell lysate were presented. Results for TNF production are shown in Figure1 (Jang et al, 2006). (Cells : PBMC: Human peripheral blood mononuclear cells, monocytes, monocytic lymphoma U-937 and leukemia…
- Based on this video: https://youtu.be/3PYdMaClUmw Observation Interpretation Test Tube 1 H2O2 + 1 piece uncooked potato + 1 mL H2O Test Tube 2 H2O2 + 1 piece cooked potato + 1 mL H2O Test Tube 3 H2O2 + 1 piece uncooked potato (cut into small pieces) + 1 mL H2O Test Tube 4 H2O2 + 1 piece uncooked potato + 1 mL vinegarSATestprep, LLC- Online State es/test/tq.php?testid-9848strandid=4936&element=D&assignment id-41828865&load test=18teacherPreview=#test A cellular protesses, maximiZing cIcIency. The ENDOSYMBIOTIC THEORY 3nascond edybee pel, he eady nukarycte ponsuned otosuhetc ngs n the plas membrane t nn ses pokaryote ge me to endonmbe comporen Incluting anuceus ns endpl um baderis roued int Endplaun cke Nudes Phot etic baceriam Modern photosynthetic ukaryote Proto-ukaryote -Meochondrim Atec bacenum Anetredon even te ancesia kaye conumed ae bic bactia fat evoived eto mnodhonca Modern heteotrophic eukanyote The diagram provided shows the evolution of membrane-bond organelles in eukaryotic cells. The Endosymbiotic Theory proposes that the organelles distinguishing eukaryote cells evolved through symbiosis of individual single-celled prokaryotes. Which of these statements provides supporting evidence for the endosymbiotic theory? A) Chloroplasts have a single phospholipid membrane. B) The DNA in…Table C. Xanthoproteic Reaction Samples |1. Tryptophan 2. Tyrosine 3. Casein 4. Albumin 5. Peptone 6. distilled water Observation
- Some Metabolic Activities of Bacteria A. Questions: 1. List 5 importance of using selected and differential culture media. 2. Mannitol Salt Agar is a differential or selective media? Explain why? 3. What enzyme and explain the principle on how Simmons citrate agar change in color for positive result? 4. What two groups of bacteria that can be differentiated with the catalase test?Selective and Differential Conditions of media and culture conditions can be used to culture and identify Listeria monocytogenes. For each, characterize it as Available Hint(s) Res Adding antibiotics, such as nalidixic acid Adding high concentrations of salts, such as lithium chloride and sodium chloride Adding a pH indicator to detect fermentation of hamnose to acid Incubate at 30°C Detecting beta-hemolysis Adding antifungals, such as amphotericin B Differential SelectiveQuestion:- How to perform a bactericidal test bactericidal test of Dental Pulp Stem Cell (DPSC)?
- Draw these steps of Qualitative Slide Test in LATEX AGGLUTINATION TEST FOR THE DETECTION OF RHEUMATOID FACTOR: SCREEN AND TITER 1. Bring all reagents and test samples to room temperature before use. 2. Make a 1:20 dilution of the patient serum by pipetting 0.1 ml of patient serum into 1.9 ml of 1X diluent or saline in a test tube. Pipet 50 µul of the diluted sample onto one test circle on the slide. 3. Place one drop (about 50 mL) of positive control onto a separate test circle and one drop of negative control onto another test circle. 4. Invert the latex reagent bottle several times to mix the beads into the suspension. Add one drop of well-shaken reagent to each test area of the slide. 5. Mix each test area with a disposable stirrer, spreading each mixture over the entire section. Use a clean disposable stirrer for each test area. 6. Rock the slide gently with a rotary motion for 2 minutes and observe immediately for agglutination.Laboratory Report Sheet – ENZYMES Procedure: Potato Peroxidase a. Prepare 3 test tubes. Place 1 mL of potato extract to each test tubes. To the first test-tube - add 5 drops of 1% phenol+ 3 drops of H2O2 second test tube – add 5 drops of catechol or pyrogallol + 3 drops H2O2 third test tube - add 5 drops of guiac solution + 3 drops H2O2 b. Shake well and observe change in color. 2. Repeat procedure 1, a, b, and c, but this time use boiled potato extract. QUESTION: WHAT ARE THE OBSERVATIONS FOR EACH TUBE?Which test tube is the control group? Test tube 1: 5 Hydrogen peroxide only Test tube 2: 5 Hydrogen peroxide and ground potato Test tube 3: 5 Hydrogen peroxide and diced potato Test tube 4: 5 Hydrogen peroxide And boiled potato