Biochemistry techniques have been used for the analysis on biomolecules such as enzymes, DNA, antibodies and other molecules in biomedical engineering. a) Polymerase chain reaction (PCR) is an artificial method to amplify DNA segment into thousand copies of that DNA. i. Elaborate the three steps in PCR which based on temperature manipulation in controlling the replication process. Distinguish the uniqyeness of Reverse Transcriptase PCR (RT-PCR) technique as compared to the common PCR.

Biochemistry
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ISBN:9781319114671
Author:Lubert Stryer, Jeremy M. Berg, John L. Tymoczko, Gregory J. Gatto Jr.
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Question 3.
Biochemistry techniques have been used for the analysis on biomolecules such as enzymes,
DNA, antibodies and other molecules in biomedical engineering.
a) Polymerase chain reaction (PCR) is an artificial method to amplify DNA segment into
thousand copies of that DNA.
i.
Elaborate the three steps in PCR which based on temperature manipulation in
controlling the replication process.
Distinguish the uniqeness of Reverse Transcriptase PCR (RT-PCR) technique as
ii.
compared to the common PCR.
iii.
Relate the application of RT-PCR and Rapid Diagnostic Test for biomarker
detection with the typical time course of COVID-19 infection.
Transcribed Image Text:Question 3. Biochemistry techniques have been used for the analysis on biomolecules such as enzymes, DNA, antibodies and other molecules in biomedical engineering. a) Polymerase chain reaction (PCR) is an artificial method to amplify DNA segment into thousand copies of that DNA. i. Elaborate the three steps in PCR which based on temperature manipulation in controlling the replication process. Distinguish the uniqeness of Reverse Transcriptase PCR (RT-PCR) technique as ii. compared to the common PCR. iii. Relate the application of RT-PCR and Rapid Diagnostic Test for biomarker detection with the typical time course of COVID-19 infection.
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