Biofilms represent an important environmental niche. A) How does growth on a surface differ from growth in a suspended laboratory culture? (In other words: How do biofilm-forming cultures differ from planktonic cultures) B) Describe the stages of biofilm development including cellular and extracellular components?
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Biofilms represent an important environmental niche.
- A) How does growth on a surface differ from growth in a suspended laboratory culture? (In other words: How do biofilm-forming cultures differ from planktonic cultures)
- B) Describe the stages of biofilm development including cellular and extracellular components?
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- Diseases that involve biofilm-producing bacteria are of serious concern. They are not as easily treated compared with those involving free-floating (or planktonic) bacteria. Explain three reasons why biofilms can be more pathogenic.Biofilms represent an important environmental niche. A) How does growth on a surface differ from growth in a suspended laboratory culture? (In other words: How do biofilm-forming cultures differ from planktonic cultures) B) Describe the stages of biofilm development including cellular and extracellular components? C) What processes regulate might regulate biofilm formation? Is quorum sensing involved and if so, how common is it? D) Why would biofilms promote the formation of genetic variants? What are the mechanisms by which this might occur? These questions are all related.In the exponential phase of microbial growth, Log cell numbers Time O a) net cell growth is equal to net cell death. Ob) net cell growth is greater than net cell death. c) waste accumulates, cell death is greater than cell growth. d) the cells stop growing.
- Bacterial growth depends on many environmental factors, including the temperature of the environment. Since microbes can survive in a certain range of temperatures and will thrive at a temperature, understanding these variables allows for control of their growth. This understanding can be used, for example, to preserve certain foods or to treat infections. This lab simulation will use an instrument called a spectrophotometer. This instrument quantitatively measures the amount of light that is absorbed or transmitted by molecules in solution a) In your own words give an introduction to the microbial growth and the effect tempature has. b) In your own words explain the importance of the spectrophotometer in regards to microbial growth. c) In your own words give a hypothesis on how bacteria will react to different temperatures.What is a chemostat? a) a chemical used as a substrate for growth of microbial cells b) a batch culture that uses a defined medium for growth c) a system used for the continuous culture of microbial cells d) a nutrient that is able to control the growth rate of a bacterial cultureDiseases that involve biofilm-producing bacteria are of serious concern. They are not as easily treated compared with those involving free-floating (or planktonic) bacteria. Explain three reasons why biofilm formers are more pathogenic. Brian goes to the hospital after not feeling well for a week. He has a fever of 38 °C (100.4 °F) and complains of nausea and a constant migraine. Distinguish between the signs and symptoms of disease in Brian’s case.
- Listen The following is a recipe for a bacterial growth medium: 35 g Dextrose, 12 g Agar, 1.2g Salt, 3 g Yeast cell extract, 20g tryptone. Based on the recipe, what type of growth medium would this be? A) Chemically defined growth medium B) Minimal growth medium C) Complex growth medium D) Chemically undefined growth medium E) More than one of the answers is correct.How do you observe growth in liquid microbiological media? a) the medium is cloudy or turbid b) colonies appear on the surface of the medium c) the medium becomes solid d) colonies appear distributes throughout the medium4. (a) Which of the following chemical reactions is an overall oxidation-reduction (redox) reaction that involves oxidation number changes in their reactions? A. NaOH + H₂O → Na¹ + H3O+ + OH B. HCl + H₂O → H3O+ + Cl- C. CO₂ + H₂O → H+ + CO3²- D. N₂ + 3H₂ → 2NH3
- Which of the following statements is a reason why Acid-Fast Bacteria resist the Gram stain a) Because their cell wall architecture varies from both gram-negative and gram-positive bacteria. b) Because they lack the peptidoglycan layer. c) Because they have a thick peptidoglycan layer. d) Because they have a thick layer of fatty acids.Why is biofilm development in catheters (tubes that can be inserted into a body cavity, duct or vessel which allow drainage or injection of fluids) such a dangerous source of infection in hospitals?a) Biofilm is: a. a complex established community of microorganisms on solid surfaces b. is a layer of millions of identical bacteria on solid surfaces c. is a layer of compounds released from the tissue as a protection against pathogenic microorganisms d. All of these are correct b) Capsule formation is associated with the virulence of pathogenic bacteria. Why? Select all that apply. a. They are sticky and attach better b. They can escape phagocytosis c. The capsule makes them grow/multiply faster d. They kill immune cells