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- A.How much of 10,000x SYBR safe would you add to 50 ml to make a final concentration of 1x? B.How will you set up the serial dilution? How many tubes do you need? What is the concentration in each? How much LB will you add to each tube? What volume of cells will you add?20 mg active ingredient in 200ml normal saline. What is the weight/ volume concentration?G.What concentration of imidazole do you expect after you dialyze 1 mL of a sample containing 500 mM imidazole into 100 mL of dialysis buffer containing no imidazole? What if you repeat the process by moving the dialysis cassette to a fresh 100 mL of dialysis buffer?
- e Mar 29 5) Order: Potassium Chloride 80 meq p.o. daily potassium Chloride 20 meq per 15 mL Supply: 150 mL Calculate the dosage in fl-oz 6) The order is for amoxicillin 500 mg PO. You have on hand a 150-mL bottle of dry powder. The directions read, "Add 105 mL of water and shake well i obtain 150 mL of suspension containing 250 mg per 5 mL." (a) How many milliliters will contain 500 mg? (b) Divided into 2 equal doses, how many milliliters will there be per dose?5 tabs po qd x4 d, taper down by one tablet every 5 d for 15 d then ss po qd. How many tablets will the patient need to take after the taper per day?A. You have 3% (w/v) concentrated saline in 20 mL ampoules. What volume and how many ampoules do you need to make 1 L of an isotonic saline solution (0.9% w/v). i) 300 mL of 3% (w/v) saline; 15 ampoules iii) 360 mL of 3% (w/v) saline; 18 ampoules 120 mL of 3% (w/v) saline; 6 ampoules 240 mL of 3% (w/v) saline; 12 ampoules iv) B. A hypothetical organism, 'Macquarie biomolecula' is an anaerobic thermophilic microbe that is found in hydrothermal vents. It lives at temperatures near the boiling point of water and at pressures over 200 times higher than at sea level. It does not contain peptidoglycan. This organism is a type of: i) Eukarya ii) Bacteria iii) Archaea iv) Protista c. Which of the following statements is correct? i) all RNA viruses are single-stranded, and all DNA viruses are double-stranded ii) a virus is a type of cell iii) a virus infects a single host species iv) retroviruses contain RNA as their genome D. Which of the following may be found in the circulation? i)…
- At 10 p.m. , a patient must receive 1g of Cefazolin (Ancef) intravenous piggyback (1.V.P.B.) in 250ml of Dextrose 5% in water (D5W), to be infused over 45 minutes. The drop factor is 10gtt = 1ml . What is the correct flow rate of this antibiotic solution? 10 qtts/min 45 qtts/min 31. 249 qtts/min 55.55 qtts/minOrder: cefoxitin sodium (Mefoxin) 200 mg IM Q4HSupply: 1g powderDiluent: 2 mL sterile waterConcentration 400 mg/mLRound to the nearest tenth Order: 1000 mL D5NS IV to run at 150 mL/hrCalculate the drops per minute (gtts/min) for each of the different tubing.a) Macrodrop (10 gtt/mL) b) Microdrip (60 gtt/mL)C. The following successive dilutions are applied to a stock solution that is 5.60 M sucrose: Solution A = 46.0 mL of the stock solution is diluted to 116 mL %3D Solution B = 58.0 mL of Solution A is diluted to 248 mL Solution C = 87.0 mL of Solution B is diluted to 287 mL 1. What is the concentration of sucrose in solution B? 2. What is the concentration of sucrose in solution C? 3. What is the dilution factor of Solution A? 4. What is the dilution factor of Solution B? 5. What is the dilution factor of Solution C?
- A. You have 3% (w/v) concentrated saline in 20 mL ampoules. What volume and how many ampoules do you need to make 1 L of an isotonic saline solution (0.9% w/v). i) 300 mL of 3% (w/v) saline; 15 ampoules 360 mL of 3% (w/v) saline; 18 ampoules 120 mL of 3% (w/v) saline; 6 ampoules 240 mL of 3% (w/v) saline; 12 ampoules iii) iv)The order is for 1000ml of 5% D/W, 1000ml of Ringers Lactate, 500ml of NS to run over a 36 hour period. The drop factor is 15gtt/ml. How many gtt/min will you administer? using dimensional analysis and show workFrom a 5% normal saline (NaCl) solution, take 2 ml and dilute it 1:5. Take 5 ml of this solution and dilute it 1:10. A 2:100 dilution is made from this last solution. Determine: a) How many times less concentrated is the 2nd dilution than the original? b) What is the dilution factor in each step of the series and what is the final volume of each dilution? c) How many grams of NaCl will be in 1 ml of the last solution?