Calculate the pl of the amino acid using the titration curve. 04 pH 0 6 8 SO 10 O 12.0 12 10.0 8.0 6.0 4.0 2.0 0.0 Histidine (pH 0) COOH H₂N-CH H CH 0.5 1.0 1.5 2.0 2.5 NaOH (equivalents) 3.0
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There are two parts to this problem.
A) the pl for Histidine using the titration curve.
Choices:
B) pKA value for Histidine usind the titration curve.
Choices, can be more than 1:
Step by step
Solved in 2 steps with 1 images
- A 100 mL of 0.1 M amino acid at pH 1.0, whose pka for the carboxyl group is less than the pKa of the R-group, was titrated with NaOH solution. The pH was monitored, and the results were plotted on a graph, as shown below. The key points in the titration are designated A to G. 12 10 8 pH 6 4 2 0 A B 0.5 с D E F 1.5 2 2.5 Equivalents of OH 1. What is the possible identity of the amino acid? [Select] 2. What is the isoelectric point of the amino acid? [Select] 3. What is the pka corresponding to the deprotonation of the alpha-amino group? [Select] 4. Region/ point where the amino acid is predominantly present as a (-2)-charged species. [Select] 5. The effective buffering range for the amino acid in the basic region. [Select] G 3Cells in the human liver contain glycogen, a polysaccharide that is used as a source of energy. This is a model of a typical glycogen granule: 42 22 AWARD Edit View Insert Format Tools Table be *** www 27544 Hals De w Jay MASCH Abh P CON p bo H S ******** 124 3. P WH ** patink The G k page flees P ******* 44651 CAPE Porn Tele de to MATE www 74 H Supe This type of granule may contain up to 30,000 (3 x 104) glucose subunits, making up the glycogen chains. A. How many ATP's could theoretically be produced when a granule of glycogen is hydrolyzed to glucose subunits that then enter glycolysis and respiration? B. Glycogen metabolism is regulated by insulin and glucagon. Both of these hormones are produced by the pancreas and act as signals that interact with a transmembrane receptor on target cells. Intracellular events involve protein kinase A that activates or inactivates enzymes involved in glycogen synthesis or degradation. Briefly describe what protein kinase A does to these proteins.Please help me calculate the molecular weight of the unknown amino acid. The following are the given data from titration: Mass: 0.2689 pI: 7.359 volume of NaPH at pI: 20 mL pKa1: 1.179 pKa2: 12.333 mass is in grams NaOH not NaPH
- Five amino acids were subjected to normal phase chromatography, and the folowing Rf values were computed: A 0.59 В - 0.12 C- 0.71 D- 0.33 E - 0.20 Which is the most polar amino acid? O A O BUse the provided theoretical titration curve of histidine in answering the questions asked. pH 14 12 10 8 6 4 2 O 0 2 4 1. In the titration curve above, what is the isoelectric point of histidine? Make sure to place your answer up until the first decimal point. 6 8 10 12 14 16 18 20 22 24 26 28 30 32 34 36 38 40 Volume of Titrant / mL Histidine 0.10 mol/L - titrant = NaOH 0.1 mol/L 2. Which form of histidine is the most abundant at pH 10.0? (and why) a. Hist b. His c. His2+ d. His 3. True or False: All amino acids have zwitterions (explain) 4. Which of the following forms of Histidine is/are amphoteric? (More than one answer may apply) (explain) a. Hist b. His c. His2+ d. HisConsider the second titration. 0 0 0 0 0 0 0 ☐☐☐ PH 100/ | 3 14.0 4 12.0 2 8.0 6.0 Select the pK, values of each functional group observed on the titration curve. 4.0 2.0 0.0 0.5 1.0 1.5 2.0 2.5 3.0 equivalents of [OH"] Identify which amino acid is being titrated. alanine aspartate histidine asparagine
- NH3* *H3N Give the abbreviation for the above tripeptide using the three-letter codes for each amino acid. Z-エ の エ-Z697149&page%3D2 ning System (Academic) 49 When we refer to the backbone of a polypeptide, we are referring to the attachment between: Select one: out of O a. The amino, carboxyl group and the oa-carbon O b. The peptide bond between two amino groups question O c. The amino acids making the polypeptide O d. The amino and carboxyl groups only O e. The R groups of the amino acids Which of the following molecules is a polymer made from large number of building blocks called monomers linked together by covalent bonds? 50 out of Select one: O a. Glucocorticoids, a steroid hormone question O b. Waxes (such as beeswax) O c. Glycogen, that stored in liver cells O d. Cooking oils O e. Stearic acid (saturated fatty acids)A 100 mL solution of 0.1 M glycine at pH 1.72 was titrated with 2 M LIOH. The pH was monitored and the result were plotted as shown in the graph. The key points of the titration are designated as I to V. Identify the key point in the titration corresponding to the pH at which the carboxyl group is half deprotonated. 12 JL30 (V) 9.60 (IV) 8 5.97 pH 6 2.34 CII) 2 (I) 0.5 1.0 1.5 2.0 OH (equivalents) IV O Il and IV Next
- 14 12 10 pH 8 6 4 0 Titration curve for an unknown amino acid pk₁=1.8 pK₂=6.0 pK, 9.2 Volume (mL) of 1 M NaOH added A student titrated a 250 mL solution of an unknown amino acid with 1.0 M sodium hydroxide and drew the below titration curve. From this titration curve they found that the amino acid had three functional groups with the pKa of the functional groups displayed on the graph. To do this titration they added in a total of 75 mL of 1.0 M sodium hydroxide. They also calculated that they added a total of 15 mL of sodium hydroxide to get to pK₁ (indicated by arrow) and a total of 37 mL of sodium hydroxide to get to pK₂(indicated by arrow). What was the initial concentration of the amino acid solution in milimolar (mM)? Provide the answer with NO decimal places. You do not have to indicate the unit in the answer field!Match each charge form of alanine under the pH condition where it would be the predominant form. The pka values for the carboxyl group and amino group of alanine are approximately 2.3 and 9.7, respectively. H H H Н.С — с — соо H Н.С — с — соо Н.С— с —соон Нс — с —соон NH, NH, NH NH, A B D v pH11 2. A Does not occur in significant amounts at any pH 3. D 4. BLook at the amino acid composition of a protein given in the table and click on the area in the 2D gel electrophoresis where you may expect the corresponding band. Amino acid composition M N P 7 3 11 A D E F G K R S V W Y ТОTAL: 16 2 12 14 4 1 3 7 2 11 11 10 1 164 Download Image. pH7 pH 9 pH3 200,000 116,250 97,400 66,200 45,000 31,000 21,500 14,400 ||| | |||