Catalase: 1. Explain the incubation conditions 2. Explain the reagents being added 3. Explain the observations in Catalase 4. Explain the interpretations in Catalase
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Catalase:
1. Explain the incubation conditions
2. Explain the reagents being added
3. Explain the observations in Catalase
4. Explain the interpretations in Catalase
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Solved in 4 steps
- Phenylananine Deaminase: 1. Explain the incubation conditions 2. Explain the reagents being added 3. Explain the observations in phenylananie deaminase 4. Explain the interpretations in phenylananie deaminaseUrease: 1. Explain the incubation conditions 2. Explain the reagents being added 3. Explain the observations in Urease 4. Explain the interpretations in Urease1. What is this lipid structure, give its role/ function, and enumerate its hydrolysis products or components. 2. Explain which hydrolyzed component will be positive in the Acrolein test? Ninhydrin test?
- A. Explain why it is essential to digest all wild-type pQE. 1.-CRYGD. B. How would you make 50 mL o 1% weight per volume (w/v) agarose solution?SIM (Indole): 1. Explain the incubation conditions 2. Explain the reagents being added 3. Explain the observations in SIM (Indole) 4. Explain the interpretations in SIM (Indole)1. Give the importance of carbohydrate fermentation test in biochemical testing and enumerate the enzymes that are involved. (5 points) 2. What are the substances added in the culture media utilized by the organism producing hydrogen sulfide? Give 2 other media used for the production of hydrogen sulfide. (5 points) 3. Explain the mechanism taking place in the hydrolysis of urea which leads to the formation of bluish red color. ( 5 points) 4. Discuss why human blood plasma will not always yield reliable results. (5 points)
- B. COLOR REACTIONS. Give the ideal observation for the positive results of the following samples to complete the table below. Write NO RXN if the sample gives negative result for the given tests BIURET XANTHOPROTEIC NINHYDRIN SULFUR SAMPLES TEST TEST TEST TEST Formation of violet- Cysteine NO RXN colored product Formation of red- Tryptophan NO RXN colored solution Formation of Formation of violet- Keratin black colored product precipitate B - Alanine NO RXN NO RXNC. d. Lactic Acid Red dye #4 Which of the following do we NOT look at when determining results of a TSI tes a. Color of the butt b. Color of the slant Liquification d. Gas bubbles/breaks in the agar CO2 released from Citrate utilization causes the media to become more a. Acidic b. Alkaline c. Neutral d. Liquid of beau mentation can result in two different end products, Aleohols, acids Ketones, bases Tryptophan, alcohol sodium caf Viviliensa r ar1. Give the importance of carbohydrate fermentation test in biochemical testing and enumerate the enzymes that are involved. 2. What are the substances added in the culture media utilized by the organism producing hydrogen sulfide? Give 2 other media used for the production of hydrogen sulfide. 3. Explain the mechanism taking place in the hydrolysis of urea which leads to the formation of bluish red color. 4. Discuss why human blood plasma will not always yield reliable results.
- PRECIPITATION OF PROTEINS 2. Give the rationale for the following: a) The use of picric acid in burns b) The use of tannic acid in diarrhea 2. List 5 agents (physical or chemical) which could be used to destroy the life of microorganisms by coagulating its protein.Oxidase Test:In the document you submit, please describe the results for this test… (like yellow equals what?) 1) Give me the expected results for Escherichia coli and Pseudomonas aeruginosa1.How is acid or alkaline detected in a culture medium as an end product? 1.The tests for the hydrolysis of starch, casein, and triglycerides are similar in terms of setup . How do the methods for the detection of hydrolysis differ between these three tests?