Colonies have grown in a nutrient agar plate. However, it did not grow along the lines of streaking. Is this normal? If not, what could be the problem?
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Colonies have grown in a nutrient agar plate. However, it did not grow along the lines of streaking. Is this normal? If not, what could be the problem?
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- After streaking microbial culture on agar plates and observing colonial growth, TMTC usually happens. What are the causes of TMTC plates (plates with more than 300 colonies that cannot be counted)? What are the ways to prevent this from happening?Describe the appearance of an S. agalactiae colony grown on blood agar. Describe how that colony would differ in appearance from a colony of S. pyogenes.Do you think Rickettsia rickettsii can grow on blood agar? Explain.
- what is the physical characteristics of this streak plate? Look at a single colony on a streak plate and look for special physical characteristics such as: motility, possible presence of endospores and/or capsule, culture color?Can you tell IF the pasteurization process is working in this milk producing plant? How can you explain this by looking at the bacterial plate growth? How can you tell it is or is not working?Why is it important to use a sterilized loop between streaks when preparing a streakplate? Observation of a streakplate culture shows more growth in Quadrant 4 than in Quadrant 3. Account for this observation. Describe the way in which you can isolate an individual colony from a spreadplate or a streakplate that holds multiple colonies. Outline the differences between a streak plate and a spread plate.
- Some exconjugants did not grow on minimal medium. On what medium would they grow?A soil sample is placed in liquid and the number of bacteria in the sample determined in two ways: (1) colony count and (2) direct microscopic count. How would the results compare?a) Methods 1 and 2 would give approximately the same results.b) Many more bacteria would be estimated by method 1.c) Many more bacteria would be estimated by method 2.d) Depending on the soil sample, sometimes method 1 would be higher and sometimes method 2 would be higher.What is the purpose of this subculture procedure? In general, how is this carried out in tissue culture maintenance?
- Which culturing method provided the clearest results, the semisolid medium tube or the soft agar plate? Explain.Describe the process to make a 4-phase streak plate beginning with your first streak (i.e you have bacterial culture on your sterile loop and are about to start to streak your agar plate ).You are given a 1 gram soil sample of unknown bacterial load. After doing 10-fold serial dilutions of the soil in sterile water, 100 uL volumes are taken from each dilution for preparation of pour plates. Following incubation, each half of the 10-8 plate has 46 colonies.a) What was the dilution factor?b) How many bacteria were present in the soil?2. Staphylococcus aureus divides every 20 minutes. A culture begins with 10 bacterial cells.a) After 5 hours, how many generations have occurredb) After 5 hours, how many bacteria are present?3. How many milliliters would you need to prepare a 10-2 dilution from a 10ml starting culture?