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Compute the cfu ml-¹ acquired from each diluent and sampling time. Fill in the table below E. coli counts Percent change in population (cfu ml-¹ +S.D.) Diluents DW DW-0.85% NaCl DW-3.0% NaCl PBS PBS-0.85% NaCl PBS-3.0% NaCl Oh 2 h

Compute the cfu ml-¹ acquired from each diluent and sampling time. Fill in the table below E. coli counts Percent change in population (cfu ml-¹ +S.D.) Diluents DW DW-0.85% NaCl DW-3.0% NaCl PBS PBS-0.85% NaCl PBS-3.0% NaCl Oh 2 h

Human Anatomy & Physiology (11th Edition)
Human Anatomy & Physiology (11th Edition)
11th Edition
ISBN:9780134580999
Author:Elaine N. Marieb, Katja N. Hoehn
Publisher:Elaine N. Marieb, Katja N. Hoehn
Chapter1: The Human Body: An Orientation
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Problem 1RQ: The correct sequence of levels forming the structural hierarchy is A. (a) organ, organ system,...
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1. To test the ability of each diluent to sustain the viability of Escherichia coli during dilution, the cells were incubated at room temperature (20 to 23°C) or 37°C for 0 or 2 h and plated on NA agarin duplicate. The counts obtained are shown in the following table: E. coli counts on dilution time O hour 2 hours Diluents 10-5 10-6 10-7 10-5 10-6 10-7 R1 26 ; 27 3:2 0:0 0:0 0:0 0:0 Distilled Water R2 31 ; 27 1:0 0:0 0:0 0:0 0:0 (DW) R3 27 : 28 4:5 0:0 0:0 0:0 0:0 R1 290 : 295 34 :31 2:1 252 : 239 27 : 26 1;1 DW-0.85% NacI R2 315 ; 309 37 : 34 2;2 250 : 245 26 ; 26 1:0 R3 301 ; 285 36 : 44 1:2 235 ; 267 34 : 21 0:0 R1 385 ; 392 42 : 35 4:5 368 :379 33 ; 32 2:2 DW-3.0% Nacı R2 370 : 388 37 : 40 2:4 305 : 303 35 ; 30 2:5 R3 355 : 365 35 : 28 4:6 370 :350 33: 37 3:1 R1 380 ; 383 30 : 27 2:2 255 : 245 25 : 24 2;2 PBS R2 341 ; 352 27 : 34 1:3 230 : 245 26 : 27 2:2 R3 329 ; 335 33 : 31 3:6 242 : 250 28 : 29 2:1 R1 340 ; 320 32 : 35 1:0 245 : 249 27 : 25 1 1 PBS-0.85% Naci R2 340 ; 345 35 : 33 3:1 245 : 245 24 : 25 2;2 R3 350 ; 365 36 : 34 1:0 250 ; 252 25 : 27 1:1 R1 340 ; 361 42 : 41 5:4 375 ; 350 40 ; 42 4: 4 PBS-3.0% Nacl R2 380 ; 409 45 : 47 4:4 365 ;375 36: 37 1;1 R3 399 ; 421 39 : 43 4: 1 350 : 365 40 : 37 4:0 Note: R1, R2, and R3 are replicates Compute the cfų ml·1 acquired from each diluent and sampling time. Fill in the table below E. coli counts (cfu ml-1 + S.D.) 2h Percent change in population (%) Diluents 0h DW DW-0.85% NaCl DW-3.0% NaCl PBS PBS-0.85% NaCl PBS-3.0% Nacl Determine the change in population of E. coli diluted and suspended in different diluents using the following formula: Percent change in population (%) N. - N, x 100 No
Transcribed Image Text:1. To test the ability of each diluent to sustain the viability of Escherichia coli during dilution, the cells were incubated at room temperature (20 to 23°C) or 37°C for 0 or 2 h and plated on NA agarin duplicate. The counts obtained are shown in the following table: E. coli counts on dilution time O hour 2 hours Diluents 10-5 10-6 10-7 10-5 10-6 10-7 R1 26 ; 27 3:2 0:0 0:0 0:0 0:0 Distilled Water R2 31 ; 27 1:0 0:0 0:0 0:0 0:0 (DW) R3 27 : 28 4:5 0:0 0:0 0:0 0:0 R1 290 : 295 34 :31 2:1 252 : 239 27 : 26 1;1 DW-0.85% NacI R2 315 ; 309 37 : 34 2;2 250 : 245 26 ; 26 1:0 R3 301 ; 285 36 : 44 1:2 235 ; 267 34 : 21 0:0 R1 385 ; 392 42 : 35 4:5 368 :379 33 ; 32 2:2 DW-3.0% Nacı R2 370 : 388 37 : 40 2:4 305 : 303 35 ; 30 2:5 R3 355 : 365 35 : 28 4:6 370 :350 33: 37 3:1 R1 380 ; 383 30 : 27 2:2 255 : 245 25 : 24 2;2 PBS R2 341 ; 352 27 : 34 1:3 230 : 245 26 : 27 2:2 R3 329 ; 335 33 : 31 3:6 242 : 250 28 : 29 2:1 R1 340 ; 320 32 : 35 1:0 245 : 249 27 : 25 1 1 PBS-0.85% Naci R2 340 ; 345 35 : 33 3:1 245 : 245 24 : 25 2;2 R3 350 ; 365 36 : 34 1:0 250 ; 252 25 : 27 1:1 R1 340 ; 361 42 : 41 5:4 375 ; 350 40 ; 42 4: 4 PBS-3.0% Nacl R2 380 ; 409 45 : 47 4:4 365 ;375 36: 37 1;1 R3 399 ; 421 39 : 43 4: 1 350 : 365 40 : 37 4:0 Note: R1, R2, and R3 are replicates Compute the cfų ml·1 acquired from each diluent and sampling time. Fill in the table below E. coli counts (cfu ml-1 + S.D.) 2h Percent change in population (%) Diluents 0h DW DW-0.85% NaCl DW-3.0% NaCl PBS PBS-0.85% NaCl PBS-3.0% Nacl Determine the change in population of E. coli diluted and suspended in different diluents using the following formula: Percent change in population (%) N. - N, x 100 No
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