5. Examine the following data obtained by a group of students:Tube Results of Lugol's iodine TestYellowResults of Benedict's TestBlue1Orange precipitateBlue2345Light yellowDark PurpleDark yellowYellowCloudy blueBlueAnswer the following questions and explain your answer.a. Which solution contains starch? 3b. Which solution is most likely glucose?c. Which solution contains the amino acid proline?d. Which solution contains an amino acid other than proline?e. Which tube probably contains only water?f. From the data provided, can you tell which tube contains fats or oils?g. From the data provided, can you tell which tube contains protein?Results of Ninhydrin TestVioletClearClearYellowClear

Introduction Starch is a polysaccharide or complex carbohydrate. It is made up of glucose monomers.…

Answered: data obtained by a group of students:…

Biochemistry

9th Edition

ISBN:9781319114671

Author:Lubert Stryer, Jeremy M. Berg, John L. Tymoczko, Gregory J. Gatto Jr.

Publisher:Lubert Stryer, Jeremy M. Berg, John L. Tymoczko, Gregory J. Gatto Jr.

Chapter1: Biochemistry: An Evolving Science

Section: Chapter Questions

Problem 1P

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Which of the following statements best describe testing of lactose sample?* A. Lactose will quickly (less than 3 minutes) form a thin brick red precipitate in a Barfoed's test since it is a reducing monosaccharide. B. Lactose will give positive test using phenylhydrazine test and forms a needle shaped osazone C. Lactose when reacted with nitric acid and exposing to cold water bath will form insoluble white crystals. D. Lactose when tested using Seliwanoff's test will form a red colored complex.
The reults for the macroscopic part: 0.30M glycerin – solution was translucent (could see text behind the test tube) 0.15M NaCl – solution was opaque (could not see text behind the test tube) 0.30M NaCl – solution was opaque (could not see text behind the test tube) 0.15M glucose – solution was translucent (could see text behind the test tube) 0.30M glucose – solution was opaque (could not see text behind the test tube) 0.30M Urea – solution was translucent (could see text behind the test tube) Results for microscopic part: 0.30M glycerin – no cells present 0.15M NaCl – normal sized cells 0.30M NaCl – crenated (shrunken and star-shaped) cells 0.15M glucose – no cells present 0.30M glucose – normal sized cells 0.30M Urea – no cells present Determine the osmolarity (hypoosmotic, isosmotic, or hyperosmotic) and tonicity (hypotonic, isotonic, hypertonic) of the following solutions.In which solutions did the osmolarity NOT match the tonicity? For those solutions, why did the osmolarity…
1. What test reagent is most sensitive in detecting sugar in urine? What are the possible positive results (color) and its interpretation. 2. What test could be used to differentiate: Explain. a. sucrose and lactose b. glucose and starch? Explain. c. glucose and fructose? Explain. d. galactose and fructose? Explain 3. An unknown carbohydrate gave a brick red precipitate when tested with Fehling's reagent, turned red when reacted with Seliwanoff's reagent, and quickly gave a red precipitate when reacted with Barfoed's reagent. What conclusions can be made about this carbohydrate? 4. Illustrate with equation the reaction of glucose, galactose, maltose & lactose with osazone test. Sketch the crystals formed for each of the following sugars examined under the microscope.
6. Complete the table below. If the sample is expected to show no reaction for a particular test, write NO RXN. If the sample is expected to show a reaction, describe the ideal observation for a positive result. Molisch's Bial's Test Iodine Test Benedict's test Cellulose Formation of purple interphase Formation of Fructose purple interphase Mannose Galactose
Millon-Nasse Test Mix 1 mL of the sample and 5 drops of Millon-Nasse reagent. Boil the content gently for 30 seconds. Cool in water. Add 2 drops of freshly prepared 1% NaNO2 and warm gently. Observe the color of the precipitate and/or the solution. Question 1. Will all amino acids and proteins give a positive reaction to this test?Question 2. What causes the color of the solution or precipitate?
1. What type of reaction occurred when the samples (enumerated) reacted with the Molisch reagent? Give the chemical equation for each.a. Glucoseb. Sucrosec. Fructosed. Lactosee. Galactosef. Ribose 2. Explain the mechanism behind the test in simple terms. 3. What are the important products of the reaction of each sample?
The table below summarizes the results for Sudan IV test. Which solvent the lipid samples are homogeneous? PUT A CHECK Hexane Ethyl Acetate Water Methanol A B C Which solvent the lipid samples are heterogeneous? PUT A CHECK Hexane Ethyl Acetate Water Methanol A B C What is the nature of the lipid samples?* A. Polar B. Nonpolar
2. Mucic acid test – place 1 ml of the sample (lactose, galactose, glucose, fructose) in separate tubes. Add 1 ml conc. HNO3 in each tube. Heat the tubes in a boiling water bath for 1 hour. Cool at room temperature. Induce crystallization by scratching the side of the tube with a clean stirring rod, if no crystals appear; let it stand until the next laboratory period. Examine the crystals under the microscope and draw.
In the third video, glucose, glycine and maltose are tested as samples with the Benedict's reagent. Test Tube COLOR CHANGE EXPLANATION 1. Glucose 2. Glycine 3. Maltose If glucose and maltose are both sugars, why is maltose not as red in color as glucose?
1. Identify what test is being described: Refers to the breaking of peptide bonds that connect amino acids to compose protein ? A. Hydrolysis B. Denaturation C. Heat denaturation D. Organic solvent denaturation E. Biuret test F. Hopkins – Cole Reaction G. Millon’s test H. Ninhydrin Test I. Sulfur test J. Xanthroproteic Test K. Chromatography L. Paper chromatography M. Competitive inhibition N. Noncompetitive inhibition O. Rancidity P. Hydrogenation 2. Identify what test is being described: Test that detects the free amino group in amino acids ? A. Hydrolysis B. Denaturation C. Heat denaturation D. Organic solvent denaturation E. Biuret test F. Hopkins – Cole Reaction G. Millon’s test H. Ninhydrin Test I. Sulfur test J. Xanthroproteic Test K. Chromatography L. Paper chromatography M. Competitive inhibition N. Noncompetitive inhibition O. Rancidity P. Hydrogenation 3. Identify what test is being described: A foreign substance, which is structurally similar to the substrate, competes for…
My question is: Could you explain steps? (etc. why do we heat or add chemicals like nacl or ammonium sulphate?) 1)Casein Isolation from MilkExperimental Procedure* Put 100 ml of milk in a 500 ml beaker and heat it to 40 0C. * Also heat 100 ml acetate buffer and slowly add it to the beaker with milk while stirring. * The final pH of the mixture should be 4.8. * Cool the suspension to room temperature and wait 5 more minutes before filtering. * Wash the precipitate several times with small volumes of water and then suspend it in approximately 30 ml of ethanol.Filter the suspension in the buchner funnel and wash the precipitate a second time with a mixture of equal proportions of ethanol and ether. * Finally, wash the precipitate on filter paper with 50 ml of ether and soak and dry. * Make the ether evaporate by spreading the powder. * Weigh the casein and calculate the percentage yield of the protein. * Compare with theoretical yield of 3.5 g per 100 ml milk. 2)Purification of Egg…
1. List down the amino acids in the following format Amino Acid Physiological Significance 3 letter Code 1 letter code Chemical Structure 2. List down the different qualitative and quantitative tests for Amino Acids Test Reagents Principle Positive Reaction/Result

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