Describe how an efficient separation of anions can be achieved using capillary zone electrophoresis (CZE). Two neutral compounds, A and B are succesfull separated by micellar electrokinetic chromatography (MEKC) using 15 mM borate buffer (pH 8) with 50 mM sodium dodecyl sulphate (SDS). i) The migration time of A and B are 8.1 and 13.0 min, respectively. Explain the hydrophobicity property of the compound based on the migration times obtained. ii) If SDS is added in a very low concentration (< 50 mM) into the system, explain the obtained separation. Give an advantage of MEKC over CZE.

Introduction to Chemical Engineering Thermodynamics
8th Edition
ISBN:9781259696527
Author:J.M. Smith Termodinamica en ingenieria quimica, Hendrick C Van Ness, Michael Abbott, Mark Swihart
Publisher:J.M. Smith Termodinamica en ingenieria quimica, Hendrick C Van Ness, Michael Abbott, Mark Swihart
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Describe how an efficient separation of anions can be achieved using capillary zone
electrophoresis (CZE).
a)
b)
Two neutral compounds, A and B are succesfull separated by micellar electrokinetic
chromatography (MEKC) using 15 mM borate buffer (pH 8) with 50 mM sodium
dodecyl sulphate (SDS).
i)
The migration time of A and B are 8.1 and 13.0 min, respectively. Explain the
hydrophobicity property of the compound based on the migration times obtained.
i) If SDS is added in a very low concentration (< 50 mM) into the system, explain
the obtained separation.
c)
Give an advantage of MEKC over CZE.
Transcribed Image Text:Describe how an efficient separation of anions can be achieved using capillary zone electrophoresis (CZE). a) b) Two neutral compounds, A and B are succesfull separated by micellar electrokinetic chromatography (MEKC) using 15 mM borate buffer (pH 8) with 50 mM sodium dodecyl sulphate (SDS). i) The migration time of A and B are 8.1 and 13.0 min, respectively. Explain the hydrophobicity property of the compound based on the migration times obtained. i) If SDS is added in a very low concentration (< 50 mM) into the system, explain the obtained separation. c) Give an advantage of MEKC over CZE.
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