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Describe the relevant features of the F factor (plasmid) that allow it to partake in conjugation.
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- The ability of F+ cells, or Hfr cells, to transfer plasmid DNA to an F- cell is properly called: transversion transformation conjugation transduction transitionDescribe the events (in words) occurring during conjugation in bacterial cells (using F plasmid transfer)Bacterial conjugation has medical consequences. For example, certain plasmids contain genes that code for toxins. The causative agent of a deadly form of food poisoning, E. coli 0157, synthesizes a toxin that causes massive bloody diarrhea and kidney failure. This toxin is now believed to have originated in Shigella, another bacterium that causes dysentery. Similarly, the growing problem of antibiotic resistance is partly attributable to the spread of antibioticresistant genes among bacterial populations. Antibiotic resistance develops because antibiotics are overused in medical practice and in livestock feeds. Suggest a mechanism by which this extensive use promotes antibiotic resistance.
- Three pairs of bacterial cells with the given genotypes undergo conjugation. Place match the genotype of each cell after conjugation to its initial genotype. F+ x F Hfr F- F' F- Answer Bank F F F+ Hfr What is the role of the F-factor in conjugation? It contains genes necessary for replication of the donor's F plasmid. can occur. It allows auxotrophic bacterial cells to survive on minimal medium so that conjugation It contains genes that force recombination between the donor and recipient chromosomes. It contains genes necessary for the formation of the pilus. O It degrades the chromosome of the recipient cell after conjugation.Describe the similarities and differences between abacterial chromosome and a plasmidThe F plasmid encodes which two functions essential for conjugation?
- True or false White colonies in apicillin-agar plates using plasmids with lacZ gene as vector indicated both successful transformation and insertionHow can i explain my answers in depth in microbology for example this question: Assume that there are horizontal gene transfers between two completely different bacterial species. In one case it is a plasmid that is transferred via conjugation, in the other case it is a part of the bacterial chromosome that is transferred via transformation. In which of the two cases is it most likely that the transferred DNA will remain and be able to function in the recipient cells? Explain the biological background to your answer . How do I break down the question so I answer it fullyChoose the statement below that is true about the characteristics of the bacterial fertility factor factor. The fertility factor carries genes responsible for uptake of naked DNA, and has several IS elements to assist plasmid integration into host cell's chromosome. O The fertility factor carries genes responsible for formation of bacteriophages, and has several IS elements to assist plasmid transfer through the sex pilus. The fertlity factor carries genes responsible for formation of sex pili, and has several IS elements to assist plasmid integration into host cell's chromosome. The fertility factor carries genes responsible for formation of sex pili, and has several IS elements to assist plasmid transfer through the sex pilus.
- Conjugation depends on direct cell-to-cell contactbetween a donor F+ carrying either a conjugative________ (the F plasmid) or an integrated conjugativeelement (as in Hfr strains), and a recipient lacking suchan element (F−).draw structure of plasmidThe properties that all plasmids share are that they1. all carry genes for antimicrobial resistance.2. are self-transmissible to other bacteria.3. always occur in multiple copies in the cells.4. code for non-essential functions.5. replicate in the cells in which they are found.a) 1, 2 b) 2, 3 c) 3, 4 d) 4, 5 e) 1, 5