DNA Extraction Using household materials Wash the fruit. Place the fruit (e.g. banana) in a zip lock plastic bag and crush it with your fist. Add the one half teaspoon of salt and one tablespoon of dishwashing liquid to the mashed fruit mixture in the bag, zip it up and squeeze it in your hands for 1 minute. Place the strainer over the small glass. Pour the mixture into the strainer. Filter the mixture into the glass. Slowly pour ice cold rubbing alcohol into the glass, until it is about half full. Let it stand until the alcohol will form a separate layer on top of the fruit filtrate. Keep the glass still at eye level; do not shake it. Watch what happens. DNA will begin to appear where t 9. Carefully Scoop out the DNA with the straightened end of the paper clip. Touch the DNA. he alcohol and filtrate layer meet Briefly answer the following questions A. Describe two reasons for DNA isolation B. In step 2, the lysis solution you added to your Eppendorf tube and placed in the warm water bath contained detergent and enzymes. What was the purpose of the detergent? What was the purpose of the proteinase K?
DNA Extraction Using household materials Wash the fruit. Place the fruit (e.g. banana) in a zip lock plastic bag and crush it with your fist. Add the one half teaspoon of salt and one tablespoon of dishwashing liquid to the mashed fruit mixture in the bag, zip it up and squeeze it in your hands for 1 minute. Place the strainer over the small glass. Pour the mixture into the strainer. Filter the mixture into the glass. Slowly pour ice cold rubbing alcohol into the glass, until it is about half full. Let it stand until the alcohol will form a separate layer on top of the fruit filtrate. Keep the glass still at eye level; do not shake it. Watch what happens. DNA will begin to appear where t 9. Carefully Scoop out the DNA with the straightened end of the paper clip. Touch the DNA. he alcohol and filtrate layer meet Briefly answer the following questions A. Describe two reasons for DNA isolation B. In step 2, the lysis solution you added to your Eppendorf tube and placed in the warm water bath contained detergent and enzymes. What was the purpose of the detergent? What was the purpose of the proteinase K?
Human Heredity: Principles and Issues (MindTap Course List)
11th Edition
ISBN:9781305251052
Author:Michael Cummings
Publisher:Michael Cummings
Chapter15: Genomes And Genomics
Section: Chapter Questions
Problem 6QP: Which of the following best describes the process of DNA sequencing? a. DNA is separated on a gel,...
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DNA Extraction Using household materials
- Wash the fruit.
- Place the fruit (e.g. banana) in a zip lock plastic bag and crush it with your fist.
- Add the one half teaspoon of salt and one tablespoon of dishwashing liquid to the mashed
fruit mixture in the bag, zip it up and squeeze it in your hands for 1 minute.
- Place the strainer over the small glass.
- Pour the mixture into the strainer. Filter the mixture into the glass.
- Slowly pour ice cold rubbing alcohol into the glass, until it is about half full.
- Let it stand until the alcohol will form a separate layer on top of the fruit filtrate.
- Keep the glass still at eye level; do not shake it. Watch what happens. DNA will begin to
appear where t 9. Carefully Scoop out the DNA with the straightened end of the paper clip.
- Touch the DNA. he alcohol and filtrate layer meet
Briefly answer the following questions
A. Describe two reasons for DNA isolation
B. In step 2, the lysis solution you added to your Eppendorf tube and placed in the warm water bath contained detergent and enzymes. What was the purpose of the detergent? What was the purpose of the proteinase K?
C.
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