Draw a magnified diagram of a TLC plate in a developing chamber and indicate the following a) developing solvent b) TLC plate c) original spot d) solvent front and e) compounds beginning to separate on the TLC plate.
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Draw a magnified diagram of a TLC plate in a developing chamber and
indicate the following a) developing solvent b) TLC plate c) original spot d) solvent front and e)
compounds beginning to separate on the TLC plate.
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- As solvent polarity increases, the Retention Factor (Rf) of compounds on the TLC plate increases. Group of answer choices True FalsePlant pigments in spinach were separated with TLC with cellulose as thestationary phase and 80/20 diethyl ether/acetone mixture as themobile phase. If needed, the solvent structures in the lab Appendix.1. Determine the Rf values of each plant component on this TLC plate.2. Arrange the plant components from most polar to most nonpolar. Please answer both 1 and 2 (picture below is the same question attached )QUESTIONS D. How will the following affect the TLC separation? a) too much sample applied. b) Stationary phase is too active. c) Forgetting to remove the plate when the solvent has reached the of the plate. top d) Having too much solvent in the developing chamber so that its surface extends above the origin. e) Polarity of the solvent being too high. 2) Which dye would be more soluble in n-propyl alcohol, the blue or the yellow? How can you tell? 3) Which compound is more strongly adsorbed on silica, ortho- or para- nitroaniline? Correlate the R, values with the structures. 76
- 5. Summarize your conclusions from the TLC simulator. Circle the correct answer in each description about TLC separation of organic compounds on silica gel plates. a) A more polar compound has a lower/higher retention factor. b) A less polar compound has a lower/higher retention factor. c) A more polar solvent causes a lower/higher retention factor. d) A less polar solvent causes a lower/higher retention factor. e) To increase the retention factor of a spot, you should add more ethyl acetate/hexane.Why Rf value is important in Chromatography? Please shortly answer at your own words. Answer should be the point (specific 4-5 lines).1) Which technique would be best to determine the amount of nitrate in drinking water? Select one. a) Electrophoresis b) UV Spectroscopy c) Reverse osmosis d) Ion Chromatography e) Atomic Absorption 2) Which technique would be best to quantitatively determine the concentration of a substance that absorbs light in the visible range? a) HPLC b) Spectroscopy c) PCR d) Ion chromatography e) Ultracentrifugation
- Paper chromatography (Answer first letter C, D, and E)Need help with a and b. A mixture of 1-hexanol, hexane, and 1-chlorohexane is injected on a gas chromatography column. a) Predict the sequence of retention times indicating which compound will have the shortest, middle, and longest retention time. b) The sample vial of (a) was not carefully sealed and left partially open for a couple hours. A GC is taken again with only two peaks realized. The first peak to appear has an area of 24 mm2 and the second peak 40 mm2. What mole% of each compound (specify the compound name) is most likely present?Question: Using a sample of aspirin for HPLC analysis. If the aspirin was contaminated (wet) with trace amounts of a solvent such as ethyl acetate, hexane, or water, would this contaminant be detected and appear as a peak in the HPLC chromatogram? Briefly explain based on the type of detector used in HPLC analysis and what type of compounds can be detected. My Answer: The HPLC analysis detects chromophores, compounds with multiple double bonds. Since water, ethyl acetate, and hexane all lack multiple pi bonds this will not be detected in the HPLC. -Question for you, is this complete (and correct), and should I include anything about UV detection? Is UV detection used in HPLC? I thought it was only used on TLC to show compunds that arent visable.
- An analgesic drug sample was analyzed using TLC to determine its probable active ingredient. The compound may contain compound A, compound B, compound C, or compound D. Five spots were made on the TLC plate, of which four are for the standard compounds (A, B, C and D) and one for the test sample. The solvent was then allowed to pass through the TLC plate. The calculated Rf values of compound A, compound B, compound C, compound D, and the drug sample were 0.24, 0.61, 0.40, 0.89, 0.38, respectively. a. What could be the active ingredient in the analgesic drug sample? Why?An analgesic drug sample was analyzed using TLC to determine its probable active ingredient. The compound may contain compound A, compound B, compound C, or compound D. Five spots were made on the TLC plate, of which four are for the standard compounds (A, B, C and D) and one for the test sample. The solvent was then allowed to pass through the TLC plate. The calculated Rf values of compound A, compound B, compound C, compound D, and the drug sample were 0.24, 0.61, 0.40, 0.89, 0.38, respectively. a. What could be the active ingredient in the analgesic drug sample? Why?b. Arrange the four standard compounds in terms of increasing polarity Show solution and explanationWhat is the result of applying too much sample to the TLC plate? Select one: Some of the sample moves down instead of up Reduced separation between the spots O The sample moves past the solvent front O The spot does not leave the origin O The sample leeches out into the elution solvent