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- Paragraph 3. If the ocular lens on a microscope has a magnification of 20OX, what is the total magnification of: a. a scanning lens with magnification of 4X b. a low-power lens with a magnification of 1oX c. a high power lens with a magnification of 40X d. an oil-immersion lens with a magnification of 100X 4. Choose how the letter "R" will appear as viewed under a microscope. R B R As viewed with the naked eye b. a. P. 5. True/False a. Begin the focusing process with the high-power objective. b. Store the microscope with the oil-immersion objective in position. c. Use tissue d. The coarse adjustment knob can be used with any objective lens. раper and water to clean the ocular and objective lenses.The significance of using immersion oil when using 1OOX objective lens is The significance of using immersion oll when uskng 100X objective lens is: It will increase the contrast by having same refractive index as the lens, loss of light by refraction, reflection and diffraction is minimized. the specimens need not be killed, fixed and stained more light is captured for better illumination, allowing the specimen to fluoresce i. il. iii. iv. A light microscope should have the following features contrast Resolution magnification fluorescence ii. iv.1. In microscopy, what could be the possible reason why we cannot completely resolve the specimen under the Oil Immersion Objective (OIO) when, in fact, it was taught that the OIO has a resolving poewer? 2. When using the OIO, we can only focus on one group or parts at a time. (that is, the other parts are blurred) Whys is it so?
- 7. The spectrophotometer is used to measure absorbance experimentally. Briefly, explain how this is accomplished.B. Write the function/s of each part of the microscope listed below. a. Eyepiece b. Draw tube c. Hemispheric prism housing d. Dust shield e. Revolving Nose Piece f. Objectives: Scanner LPO HPO ΟΙΟ g. Arm h. Coarse Adjustment knob i. Fine adjustment knob j. Slide holder & clip k. Rear knob I. Front knob m. Central aperture n. Condenser o. Iris diaphragm p. Mechanical Stage q. Mirror r. Mirror rack s. Stage adjustment knob t. Base3. Using the information provided, calculate the size of the objects viewed. Hint you will need to calculate the FOV. A.The cell is being viewed under high power. Scanning power objective=4x; Low power objective= 10x; High power objective= 40x; Eyepiece= 5x; Low power field of view (FOV)= 4.2 mm
- 2. Complete Table 1 below by supplying the characteristics of each objective. Table 1. Numerical characteristics of microscope objectives. Focal length (mm) Working distance (mm) Linear magnification (X) Numerical aperture (N.A.) Features (color of the band, diameter of the opening of the lens, etc.) LPO HPO ΟΙΟ3. Using the information provided, calculate the size of the objects viewed. Hint you will need to calculate the FOV. b. These cells are being viewed under high power. Use the length of just one cell to estimate the number of cells that can fit into the FOV. Scanning power objective = 5X; Low power objective = 40X; High power objective = 100X; Eyepiece = 10X; Low power field of view (FOV) = 1.5 mm3. Using the information provided, calculate the size of the objects viewed. Hint you will need to calculate the FOV. b.These cells are being viewed under high power. Use the length of just one cell to estimate the number of cells that can fit into the FOV. Scanning power objective = 5X; Low power objective = 40X; High power objective = 100X; Eyepiece = 10X; Low power field of view (FOV) = 1.5 mm
- Image A: Image B: 25 um O A. image A- scanning electron microscope, Image B- phase contrast microscope O B. Image A brightfield microscope; Image B- darkfield microscope O C.image A- brightfield scanning microscope Image B- phase contrast scanning microscope D. image A- transmission electron microscope, Image B- scanning electron microscope O E. image A- scanning electron microscrope, Image B- transmission electron microscope3. Using the information provided, calculate the size of the objects viewed. Hint you will need to calculate the FOV. A. The cell is being viewed under high power. (the pink specimen picture belongs to this question) Scanning power objective=4x; Low power objective= 10x; High power objective= 40x; Eyepiece= 5x; Low power field of view (FOV)= 4.2 mm B. These cells are being viewed under high power. Use the length of just one cell to estimate the number of cells that can fit into the FOV. (the two yellow circle specimen picture belongs to this question) Scanning power objective = 5X; Low power objective = 40X; High power objective = 100X; Eyepiece = 10X; Low power field of view (FOV) = 1.5 mmAs with standard phase contrast microscopy,differential-interference contrast microscopy depends on differences in the ___________________ of a speciment to create high-contrast images. a. refractive index b. light absorption c. natural fluorescence d. walls and coverings e. natural pigmentation