Fluorescence confocal microscopy (FCM) - STK38 monoclonal antibody (M01), clone 2G8- 1F3. 200 μm a. What is the basic principle of image formation using this microscopy technique? b. What can be observed and concluded from the image of the specimen?
Q: you add radioactive thymine to a culture of eukaryotic cells that are all currently in S phase.…
A: Techniques for radioactive labelling are crucial for understanding how bacteria make DNA. Because…
Q: Identify stages of mitosis for each picture
A: The above cells are the mitosis stage of apical meristem of the onion root, apical meristem is an…
Q: A protein that binds to a ribosome binding site and prevents a ribosome from recognizing a start…
A: Ribosomes are the site for protein synthesis.
Q: Which of the following is a stimulus for insulin secretion? 1) increased blood insulin 2) decreased…
A: Pancreas is an example of heterocrine gland. Because it works as exocrine as well as endocrine. The…
Q: escribe the different cell configurations and layering patterns found in epithelial tissue.
A: The exterior of the body is covered in epithelial tissue, which also coats cavities, blood and…
Q: Identify the major function of endoplasmic reticulum.
A: Endoplasmic reticulum (ER) is the single mebrain bound, irregular network of tubules and vesicles…
Q: From the list below identify the most suitable software used to visualize a change in amino acid on…
A: Understanding the relationship between the protein sequence and its structure helps us to find the…
Q: bout the actual parents of three of the babies in (B2) has type AB- blood. Baby 3 (B3) has type ypes…
A: Introduction There four different blood groups are found in human. Blood group A, B, AB, and O.…
Q: The movement of water along a concentration gradient through a semi-permeable membrane:
A: This question is based on water transportation and about the proteins.
Q: Activated B-Cells Clustered T-Cells Antigen Antibody
A: Immunoglobulin is made up of two words immune and globulin. The word immuno means immune response…
Q: The reason why scarring is not a result of epidermal wound healing.
A: Our skin is made up of two layers. The epidermis, or outer layer, serves as a barrier to the…
Q: Which of the following is NOT true about poliomyelitis? None of the other four answers (All are true…
A: Poliomyelitis or polio is a type of disability and life-threatening disease caused by the…
Q: Describe the elements that make aged skin more susceptible to infection.
A: Aging is a natural process that happens to everyone. Aging is a continuous process that starts at…
Q: Describe one way in which the quality of human life is based on the quality of a natural ecosystem
A: Ecosystem is a part of level of organization which is formed by grouping of various communities . It…
Q: A biologist is studying a disease in corn that seems to be more common in cornfields in warmer…
A: Maize, Zea mays or corn belongs to the Poaceae family and comes under an important crop grown all…
Q: Of the following types of fish, which would be the safest to eat? Explain your answer. Mackerel,…
A: Heavy toxic metals accumulate in the fatty tissues of the organisms of a food chain.
Q: Forked bristles, miniature wings, and sable bodies are all homozygous recessive traits whose genes…
A: According to the answering guidelines, I'm going to answer 1st 2 part (A, B ) only. For the answer…
Q: What is a retroviral modification ?
A: Retroviruses are a type of virus in the viral family called Retroviridae. They use RNA as their…
Q: Describe which muscle types have striated tissue? Which is fluid?
A: The soft tissues that make up muscles and allow them to contract are known as muscular tissues. They…
Q: II. Fill in the blanks to complete the order of the hierarchical organization of living things.…
A: Introduction Cells are the structural and functional unit of life. Cells contain nucleus, various…
Q: Describe the locations of the different types of membranes in the body. What are they used for?
A: Introduction: Bodily membranes are thin sheets of cells or tissues, covering the surface of the…
Q: Determine the primary function of membrane proteins.
A: Numerous types of proteins with varied activities are present in the plasma membrane. Due to its…
Q: Explain the main difference between the 1st and 2nd lines of defense (innate immunity)
A: Our immune system comprises of three basic lines of defenses - first, second and third line of…
Q: A. What part of aerobic respiration produces the most ATP? Describe how this process works in…
A: Dnuring photosynthesis, chlorophyllous cells trap solar chemical energy is stored as bond energy in…
Q: Give examples of the relationship between ecology and evolution and discuss them.
A: Evolution is the slow shift in the inherited traits of natural populations over many generations.…
Q: The definition of "tattoo" and the potential drawbacks of body piercing.
A: The skin is made up of receptors that impart colour by covering. Melanin, haemoglobin, and…
Q: When we discuss PCR and other similar techniques, the term Tm is often used. This refers to a.The…
A: PCR ( Polymerase Chain Reaction) is a technique in which numerous copies of genetic material (…
Q: 1) gestational diabetes I 2) Type IA 3) Type IB 4) Type II 5) MODY т D
A: Diabetes It is defined as a chronic disease that is caused when the pancreas is not able to produce…
Q: How would the removal of the TATA box in a eukaryotic gene impact transcription? Group of answer…
A: A gene is the stretch of DNA that codes for a polypeptide.
Q: Name the function of ciliated pseudostratified columnar? 1. Differentiation and Ossification 2.…
A: Introduction The human body undergoes metaplasia(conversion of one cell type to other) in response…
Q: When DNA is "denatured" for sequencing, what is actually happening? The DNA is hybridized to…
A: Introduction Deoxyribonucleic acid is a polymer made of two polynucleotide chains that coil around…
Q: Which modification of eukaryotic mRNA is likely absent if the mRNA can leave the nucleus but cannot…
A: mRNAs are formed in the form of primary transcripts.
Q: Explain with the examples of biochemical test that are used to identify the bacteria in the…
A: The smallest organism that is usually defined that the are been can be seen with the naked eye is…
Q: Please answer fast 1.Distinguish between drug toxicity and allergic reactions to drugs. 2. List…
A: 1.Distinguish between drug toxicity and allergic reactions to drugs. 2. List five cellular or…
Q: a) Which of the two molecules that are the same size will passively diffuse across a cell membrane…
A: A) water and carbon dioxide can passively move across membrane because of its small size and water…
Q: morphology and stain reaction of Toxoplasma gondii
A: Introduction Parasites are the organisms that live on another organism called a "host" for…
Q: Which of the following phyla or classes displays the greatest number of species? Question 1…
A: Species is defined as the group of organisms having ability to interbreed with them and produce…
Q: What selection is exemplified by male birds of the same species having more colorful plumage than…
A: Introduction: In biology, selection is the preferable survival and reproduction—or the preferential…
Q: Gram-Negative rods, Facultative anaerobe, Non-lactose fermeting, Oxidase-negative O Escherichia…
A: Gram negative:- The crystal violet stain used in the Gram staining method of bacterial…
Q: The wobble position of a codon can sometimes vary without changing the amino acid. is where RNA…
A: Introduction The third base of the codon is called as wobble position of a codon. tRNA is the…
Q: Chefs often place salad greens in water to make the vegetables crispy. What organelles are…
A: Chefs often place salad greens in water to make the vegetables crispy. What organelles are…
Q: Describe what kinds of information can be learned from a single fossil, including information about…
A: A fossil is any preserved remains, impression, or trace of any once-living thing from a past…
Q: Discuss strategies that could be used to develop a vaccine that is more efficacious against the…
A: COVID It is a positive-strand RNA virus that belongs to the Coronaviridae family. This virus targets…
Q: The strain of λ phage t is cI857. That tells you that the cI DNA segment is disabled by a specific…
A: Lambda phage exhibits a lytic or lysogenic cycle depending on the expression of certain genes.
Q: Identify the following: a. the sex cell produced by the female b. cell division in which two cells,…
A: Since we only answer up to 3 sub-parts, we’ll answer the first 3. Please resubmit the question and…
Q: The benefit of some cellular organelles enclosed in a bilayer membrane identical to the plasma…
A: A membrane made of lipid bilayers encloses the cell's organelles. Cell signalling with the aid of…
Q: Multiple choice: Transposable elements can do all of the following except move from one position…
A: A transposable element is a DNA sequence that has the ability to change it's position within a…
Q: Determine what happens as if the spindle fiber failed to form in a cell during the process of…
A: Ans- Spindle fiber are extremely necessary for cell division. These are the protein structure that…
Q: Briefly discuss THREE (3) nucleic acid-based methods useful for determination of the diversity and…
A: Before the advancement of detection methods, traditional methods ranging from plate counting to…
Q: The social setting in which it's used has the strongest impact on the effects of which drugs?
A: Answer:- Past these overall clarifications of why individuals use drugs, humanistic conversations of…
Step by step
Solved in 2 steps
- Calculate the scan time for a GRE with TR = 30 msec, NEX = 2, Ny = 256, for (a) a single slice and (b) 15 slices.During incubation, prepare dilutions of the standard antiserum which constitutes the standard curve for the assay (concentration in ng/mL). Add 500 µL of PBS-milk to the supplied microtube containing 500 µL of standard antiserum to obtain Standard 1 [500 ng/mL]. Identify seven microtubes for standards 2 to 7 and place 500 µL of PBS-milk in each. Calculate how much of antiserum is used in each standard ?why does a GC rich (70-80%) and GC poor (30-40%) give a low read coverage?
- A phagehunter performs a spot titer using standard techniques (3 ul of each dilution spotted to lawn) of a lysate obtained from an optimum webbed plate experiment. The phagehunter counts 8 plaques on the 10-7 dilution spot. Which of the following scenarios is the best choice for the phagehunter to do next? a.) The phagehunter has not achieved a high enough titer lysate to move forward with characterization experiments, so they should go back to the pick-a-plaque experiment. b.) The phagehunter has not achieved a high enough titer lysate to move forward with characterization experiments, so they should adopt a phage from direct isolation. c.) The phagehunter has not achieved a high enough titer lysate to move forward with characterization experiments, so they should try to make more optimum webbed plates. d.) The phagehunter has achieved a high enough titer lysate to move forward with characterization experiments.A phagehunter performs a spot titer using standard techniques (3 ul of each dilution spotted to lawn) of a lysate obtained from an optimum webbed plate experiment. The phagehunter counts 4 plaques on the 10-8 dilution spot. Which of the following scenarios is the best choice for the phagehunter to do next? a.) The phagehunter has not achieved a high enough titer lysate to move forward with characterization experiments, so they should try to make more optimum webbed plates. b.) The phagehunter has not achieved a high enough titer lysate to move forward with characterization experiments, so they should adopt a phage from direct isolation. c.) The phagehunter has achieved a high enough titer lysate to move forward with characterization experiments. d.) The phagehunter has not achieved a high enough titer lysate to move forward with characterization experiments, so they should go back to the pick-a-plaque experiment.Download BLOSUM30 and BLOSUMB0 substitu- tion matrices and place them side by side on your computer screen. What are the differences between the two matrices? Why do you see these differences?
- Fluorescence in-situ hybridisation (FISH) analysis can be performed on fixed pathological tumour sections. Briefly outline why interphase FISH is used on fixed material from a solid tumour, rather than metaphase FISHWhat is the attached enzyme in this assay and its corresponding substrate? A. Horseradish peroxidase and OPD (o-phenylenediamine dihydrochloride) B. Horseradish peroxidase and PNPP (p-Nitrophenyl Phosphate, Disodium Salt) C. Horseradish peroxidase and TMB (3,3',5,5'-tetramethylbenzidine) D. Horseradish peroxidase and ABTS (2,2'-azinobis-3-ethylbenzothiazoleine-6- sulfonic acid)Besides agarosc gel electrophoresis, there is also SDS-PAGE for separation of macromolccules. Differentiate both these methods.
- In STR typing, the homozygous condition will create a higher peak than the heterozygous condition in terms of relative fluorescence. How do the capillary electrophoresis and the electropherogram correlate?question: Can you summarize and explain for me what you want to tell in the article below? When I read it myself, I do not understand exactly what is meant by the article. It would be nice if you could highlight the important points. You can use them in a figure or diagram to explain. thank you and hava a nice day :) Article: Nanomaterial-Based Vaccine Development and Immunomodulation Following the publication of the genetic sequence of SARS-CoV-2 on January 11, 2020, intense research efforts have been devoted to developing a vaccine against COVID-19. With unprecedented speed, this extraordinary scientific mobilization led the first vaccine candidate to enter the Phase I human clinical trial on March 16, 2020, and other novel candidates are rapidly following. Up to May 22, 2020, there are 10 COVID-19 candidate vaccines in clinical evaluations and 114 in preclinical development. Concerning vaccine and immunization research, nanomaterials can assist in multiple ways to boost the…Among various dye-based assays like Biuret, Lowry, Bicinchronic Acid, and Biuret Protein Assays, what can be done if a concentrated sample is out of the detection range of chosen dye-based assay?