For the chloroplast, the thylakoid membrane separates the thylakoid lumen from the stroma; the protons from the photosystems are transported, like with the mitochondrial inner membrane, against the gradient from the lumen to the stroma. However, the pH(lumen) – pH (stroma) is much larger, about 3.4. Calculate delta psi (the electric potential) for the thylakoid membrane, given that ∆G of proton transport is about the same as for the mitochondrion. Then comment on its permeability to ions, compared to the mitochondrial inner membrane’s permeability to ions.

Biochemistry
9th Edition
ISBN:9781305961135
Author:Mary K. Campbell, Shawn O. Farrell, Owen M. McDougal
Publisher:Mary K. Campbell, Shawn O. Farrell, Owen M. McDougal
Chapter8: Lipids And Proteins Are Associated In Biological Membranes
Section: Chapter Questions
Problem 39RE: REFLECT AND APPLY Which statements are consistent with the known facts about membrane transport? (a)...
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For the chloroplast, the thylakoid membrane separates the thylakoid lumen from the stroma; the protons from the photosystems are transported, like with the mitochondrial inner membrane, against the gradient from the lumen to the stroma. However, the pH(lumen) – pH (stroma) is much larger, about 3.4. Calculate delta psi (the electric potential) for the thylakoid membrane, given that ∆G of proton transport is about the same as for the mitochondrion. Then comment on its permeability to ions, compared to the mitochondrial inner membrane’s permeability to ions.
Objective: Perform calculations about the free energy change and electric potential
across various membranes, illustrating differences between them.
1. Recall the membrane proton-transport free energy equation:
AG = 2.3 RT [pH(side 1) - pH (side 2)] + Z FAY
=
where Z = the charge on the proton (+1), F = Faraday's constant = 96485 C/mol and R
gas constant = 8.314 J/K mol.
For the inner membrane of a mitochondrion, AY = + 0.168 V; use T = 310K (37°C) and
pH(matrix) - pH (intermembrane space) = 0.75.
Transcribed Image Text:Objective: Perform calculations about the free energy change and electric potential across various membranes, illustrating differences between them. 1. Recall the membrane proton-transport free energy equation: AG = 2.3 RT [pH(side 1) - pH (side 2)] + Z FAY = where Z = the charge on the proton (+1), F = Faraday's constant = 96485 C/mol and R gas constant = 8.314 J/K mol. For the inner membrane of a mitochondrion, AY = + 0.168 V; use T = 310K (37°C) and pH(matrix) - pH (intermembrane space) = 0.75.
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